富炭硅肥对水稻土铁还原菌群落特征的影响

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1354 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要为了探究富炭硅肥施加处理对稻田土壤铁还原菌相对丰度、多样性及群落结构的影响，以福州平原稻田为研究对象，分别设置对照组及3种不同剂量（300，600和900kg/hm²）富炭硅肥施加处理组.研究结果表明：施加富炭硅肥有助于土壤铁还原菌的生长和繁殖，其中600kg/hm²施加处理对铁还原菌的生长影响最为显著（P<0.05）.由Shannon指数可知，3种施加处理均使晚稻拔节期土壤铁还原菌多样性有所下降，以300kg/hm²施加处理组多样性指数降低最为明显，较对照组降低了29.4%.在早、晚稻拔节期土壤中共鉴定出5个菌门，其中变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）的占比之和平均高达95%以上，是稻田土壤中的优势菌门.施加富炭硅肥后，早稻拔节期土壤中拟杆菌属（Bacteroides）的相对丰度显著提高（P<0.05），而厌氧粘细菌属（Anaeromyxobacter）的相对丰度则有所降低.此外，环境因子与稻田土壤铁还原菌的相对丰度具有相关性，Desulfovibrio的相对丰度与土壤pH值呈显著正相关（P<0.05），Anaeromyxobacter的相对丰度与土壤温度（ST）呈显著正相关（P<0.05）.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	林美芬
	郑毅
	王晓彤
	许旭萍
	王维奇

关键词 ：富炭硅肥, 铁还原菌, 多样性, 群落结构, 稻田

Abstract：In order to explore the relative abundance, diversity and community structure of iron-reducing bacteria (FeRB) in paddy fields of Fuzhou Plain, by setting up the control group and the treatment groups with three dosage levels (300, 600 and 900kg/hm²) of carbon-enrich silicon fertilizer (CESF). The results showed that the application of CESF facilitated the growth and reproduction of FeRB, among which 600kg/hm² treatment had the most significant impact on the growth of FeRB (P<0.05). According to the Shannon index, the diversity of FeRB was decreased by the three treatment groups during the jointing stage of late rice. The diversity index of the 300kg/hm² treatment group decreased most significantly, which was 29.4% lower than the control group. In the jointing stage of early and late rice, a total of 5 phyla have been identified in the soils. The total proportion of Proteobacteria, Bacteroidetes and Firmicutes was over 95% on average, and they were the dominant bacteria phyla in paddy fields. After application of CESF, the relative abundance of Bacteroides in the soil at early rice jointing stage was significantly increased (P<0.05), while that of Anaeromyxobacter was decreased. In addition, environmental factors were correlated with relative abundance of FeRB in the soils. The relative abundance of Desulfovibrio was significantly and positively correlated with soil pH (P<0.05). The relative abundance of Anaeromyxobacter was significantly and positively correlated with soil temperature (P<0.05). The above studies concluded that diversity and community structure of FeRB were significantly affected by CESF in paddy fields.

Key words： carbon-enrich silicon fertilizer iron-reducing bacteria diversity community structure paddy field

收稿日期: 2020-08-28

PACS:

X53

基金资助:国家自然科学基金资助项目（42077086；41571287）；福建省公益类科研院所基本科研专项（2018R1034-1）

通讯作者: 郑毅,副教授,eyizheng@fjnu.edu.cn;许旭萍,教授,xuping@finu.edu.cn E-mail: eyizheng@fjnu.edu.cn;xuping@finu.edu.cn

作者简介: 林美芬(1995-),女,福建泉州人,福建师范大学硕士研究生,主要从事环境微生物研究.

引用本文:

林美芬, 郑毅, 王晓彤, 许旭萍, 王维奇. 富炭硅肥对水稻土铁还原菌群落特征的影响[J]. 中国环境科学, 2021, 41(4): 1778-1789. LIN Mei-fen, ZHENG Yi, WANG Xiao-tong, XU Xu-ping, WANG Wei-qi. Effects of carbon-enrich silicon fertilizer on the community characteristics of iron-reducing bacteria in paddy fields. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(4): 1778-1789.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I4/1778

[1]	Lovley D R, Chapelle F H. Deep subsurface microbial processes[J]. Reviews of Geophysics, 1995,33(3):365-381.
[2]	Sung Y, Fletcher K E, Ritalahti K M, et al. Geobacter lovleyi sp. nov. strain S Z, a novel metal-reducing and tetrachloroethene-dechlorinating bacterium[J]. Applied and Environmental Microbiology, 2006,72(4):2775-2782.
[3]	Amos B K, Sung Y, Fletcher K E, et al. Detection and quantification of Geobacter lovleyi strain SZ:implications for bioremediation at tetrachloroethene and uranium-impacted sites[J]. Applied and Environmental Microbiology, 2007,73(21):6898-6904.
[4]	Xu J X, Li X M, Sun G X, et al. Fate of labile organic carbon in paddy soil is regulated by microbial ferric iron reduction[J]. Environmental Science & Technology, 2019,53(15):8533-8542.
[5]	Hori T, Müller A, Igarashi Y, et al. Identification of iron-reducing microorganisms in anoxic rice paddy soil by 13C-acetate probing[J]. The ISME Journal, 2010,4(2):267-278.
[6]	Chan K Y, Van Z L, Meszaros I, et al. Agronomic values of greenwaste biochar as a soil amendment[J]. Soil Research, 2008,45(8):629-634.
[7]	黄超,刘丽君,章明奎.生物质炭对红壤性质和黑麦草生长的影响[J]. 浙江大学学报, 2011,37(4):439-445. Huang C, Liu L J, Zhang M K. Effects of biochar on properties of red soil and ryegrass growth[J]. Journal of ZheJiang University, 2011, 37(4):439-445.
[8]	Van Z L, Kimber S, Morris S, et al. Effects of biochar from slow pyrolysis of papermill waste on agronomic performance and soil fertility[J]. Plant and Soil, 2010,327(1/2):235-246.
[9]	卜晓莉,薛建辉.生物炭对土壤生境及植物生长影响的研究进展[J]. 生态环境学报, 2014,23(3):535-540. Bu X L, Xue J H. Biochar effects on soil habitat and plant growth:A review[J]. Ecology and Environmental Sciences, 2014,23(3):535-540.
[10]	赵学通,包立,康宏宇,等.秸秆生物炭对亚热带葡萄园土壤性质的影响[J]. 中国农学通报, 2015,31(6):104-108. Zhao X T, Bao L, Kang H Y, et al. Straw biochar influence on soil properties quality for subtropical vineyards[J]. Chinese Agricultural Science Bulletin, 2015,31(6):104-108.
[11]	Gorovtsov A, Minkina T, Mandzhieva S, et al. The mechanisms of biochar interactions with microorganisms in soil[J]. Environmental Geochemistry and Health, 2019,42(8):2495-2518.
[12]	Wang W Q, Lai D Y F, Li S C, et al. Steel slag amendment reduces methane emission and increases rice productivity in subtropical paddy fields in China[J]. Wetlands Ecology and Management, 2014,22(6):683-691.
[13]	Ali M A, Oh J H, Kim P J, et al. Evaluation of silicate iron slag amendment on reducing methane emission from flood water rice farming[J]. Agriculture Ecosystems and Environment, 2008,128(1):21-26.
[14]	王妙莹,许旭萍,王维奇,等.炉渣与生物炭施加对稻田温室气体排放及其相关微生物影响[J]. 环境科学学报, 2017,37(3):1046-1056. Wang M Y, Xu X P, Wang W Q, et al. Effect of slag and biochar amendment on greenhouse gases emissions and related microorganisms in paddy fields[J]. Acta Scientiae Circumstantiae, 2017,37(3):1046-1056.
[15]	Wang W Q, Lai D Y F, Abid A A, et al. Effects of steel slag and biochar incorporation on active soil organic carbon pools in a subtropical paddy field[J]. Agronomy, 2018,8(8):135.
[16]	王晓彤,许旭萍,王维奇.炉渣与生物炭施加对稻田土壤铁还原菌群落结构及甲烷排放影响[J]. 中国环境科学, 2019,39(6):2495-2505. Wang X T, Xu X P, Wang W Q, Slag and biochar application on community structure and methane emission of iron-reducing bacteria in paddy soil[J]. China Environmental Science, 2019,39(6):2495-2505.
[17]	马永跃,仝川,王维奇,等.浮萍对福州平原稻田CH4和N2O排放的影响分析[J]. 中国生态农业学报, 2012,20(6):723-727. Ma Y Y, Tong C, Wang W Q, et al. Effect of azolla on CH4 and N2O emissions in Fuzhou Plain paddy fields[J]. Chinese Journal of Eco Agriculture, 2012,20(6):723-727.
[18]	Wang W Q, Lai D Y F, Wang C, et al. Effects of inorganic amendments, rice cultivars and cultivation methods on greenhouse gas emissions and rice productivity in a subtropical paddy field[J]. Ecological Engineering, 2016,95:770-778.
[19]	鲁如坤.土壤农业化学分析方法[M]. 北京:中国农业科技出版社, 1999. Lu R K. Soil agricultural chemical analysis method[M]. Beijing:China Agricultural Science and Technology Press, 1999.
[20]	陈哲,陈春兰,秦红灵,等.化肥对稻田土壤细菌多样性及硝化、反硝化功能菌组成的影响[J]. 生态学报, 2009,29(11):6142-6147. Chen Z, Chen C L, Qin H L, et al. Effect of fertilization on bacterial community,genetic diversity of amoA and nosZ genes in paddy soil[J]. Acta Ecologica Sinica, 2009,29(11):6142-6147.
[21]	Lehmann J, Da Silva J P, Steiner C, et al. Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin:fetilizer, manure and charcoal amendments[J]. Plant and Soil, 2003,249(2):343-357.
[22]	Topoliantz S, Ponge J F, Ballof S. Manioc peel and charcoal:a potential organic amendment for sustainable soil fertility in the tropics[J]. Biology and Fertility of Soils, 2005,41(1):15-21.
[23]	Ali M A, Sattar M A, Islam M N, et al. Integrated effects of organic, inorganic and biological amendments on methane emission, soil quality and rice productivity in irrigated paddy ecosystem of Bangladesh:field study of two consecutive rice growing seasons[J]. Plant and Soil, 2014,378(1):239-252.
[24]	Zheng J, Chen J, Pan G, et al. Biochar decreased microbial metabolic quotient and shifted community composition four years after a single incorporation in a slightly acid rice paddy from southwest China[J]. Science of The Total Environment, 2016,571:206-217.
[25]	Ahn C, Peralta R M. Soil bacterial community structure and physicochemical properties in mitigation wetlands created in the Piedmont region of Virginia (USA)[J]. Ecological Engineering, 2009,35(7):1036-1042.
[26]	Stewart C E, Zheng J Y, Botte J, et al. Co-generated fast pyrolysis biochar mitigates green-house gas emissions and increases carbon sequestration in temperate soils[J]. GCB Bioenergy, 2013,5(2):153-164.
[27]	孔丝纺,姚兴成,张江勇,等.生物质炭的特性及其应用的研究进展[J]. 生态环境学报, 2015,24(4):716-723. Kong S F, Yao X C, Zhang J Y, et al. Review of characteristics of biochar and research progress of its applications[J]. Ecology and Environmental Sciences, 2015,24(4):716-723.
[28]	Prayogo C, Jones J E, Baeyens J, et al. Impact of biochar on mineralisation of C and N from soil and willow litter and its relationship with microbial community biomass and structure[J]. Biology and Fertility of Soils, 2014,50(4):695-702.
[29]	Xu H, Wang X, Li H, et al. Biochar impacts soil microbial community composition and nitrogen cycling in an acidic soil planted with rape[J]. Environmental Science & Technology, 2014,48(16):9391-9399.
[30]	王超,郭坚华,席运官,等.拮抗细菌在植物病害生物防治中应用的研究进展[J]. 江苏农业科学, 2017,45(18):1-6. Wang C, Guo J H, Xi Y G, et al. Advances in the application of antagonistic bacteria in biological control of plant diseases[J]. Jiangsu Agricultural Sciences, 2017,45(18):1-6.
[31]	刘明,李忠佩,路磊,等.添加不同养分培养下水稻土微生物呼吸和群落功能多样性变化[J]. 中国农业科学, 2009,42(3):1108-1115. Liu M, Li Z P, Lu L, et al. Changes in soil respiration and microbial functional diversity of paddy soil under different fertilizer amendments[J]. Scientia Agricultura Sinica, 2009,42(3):1108-1115.
[32]	王国兵,阮宏华,唐燕飞,等.森林土壤微生物生物量动态变化研究进展[J]. 安徽农业大学学报, 2009,36(1):100-104. Wang G B, Ruan H H, Tang Y F, et al. A review on the dynamics of soil microbial biomass in forest ecosystems[J]. Journal of Anhui Agricultural University, 2009,36(1):100-104.
[33]	杨宁,杨满元,雷玉兰,等.紫色土丘陵坡地土壤微生物群落的季节变化[J]. 生态环境学报, 2015,24(1):34-40. Yang N, Yang M Y, Lei Y L, et al. Seasonal variations of soil microbial communities on sloping-land with purple soils[J]. Ecology and Environmental Sciences, 2015,24(1):34-40.
[34]	Cruz A F, Hamel C, Hanson K, et al. Thirty-seven years of soil nitrogen and phosphorus fertility management shapes the structure and function of the soil microbial community in a Brown Chernozem[J]. Plant and Soil, 2009,315(1/2):173-184.
[35]	王伟华,刘毅,唐海明,等.长期施肥对稻田土壤微生物量、群落结构和活性的影响[J]. 环境科学, 2018,39(1):430-437. Wang W H, Liu Y, Tang H M, et al. Effects of long-term fertilization regimes on microbial biomass,community structure and activity in a paddy soil[J]. Environmental Science, 2018,39(1):430-437.
[36]	Kumar U, Shahid M, Tripathi R, et al. Variation of functional diversity of soil microbial community in sub-humid tropical rice-rice cropping system under long-term organic and inorganic fertilization[J]. Ecological Indicators, 2017,73:536-543.
[37]	Cui J, Li Y, Wang C, et al. Characteristics of the rhizosphere bacterial community across different cultivation years in saline-alkaline paddy soils of Songnen Plain of China[J]. Canadian Journal of Microbiology, 2018,64(12):925-936.
[38]	张晶,林先贵,刘魏魏,等.土壤微生物群落对多环芳烃污染土壤生物修复过程的响应[J]. 环境科学, 2012,33(8):2825-2831. Zhang J, Lin X G, Liu W W, et al. Response of soil microbial community to the bioremediation of soil contaminated with PAHs[J]. Environmental Science, 2012,33(8):2825-2831.
[39]	徐鹏霞,韩丽丽,贺纪正,等.非共生生物固氮微生物分子生态学研究进展[J]. 应用生态学报, 2017,28(10):3440-3450. Xu P X, Han L L, He J Z, et al. Research advance on molecular ecology of asymbiotic nitrogen fixation microbes[J]. Chinese Journal of Applied Ecology, 2017,28(10):3440-3450.
[40]	潘孝晨,唐海明,肖小平,等.不同土壤耕作方式下稻田土壤微生物多样性研究进展[J]. 中国农学通报, 2019,35(23):51-57. Pan X C, Tang H M, Xiao X P, et al. Paddy soil microbial diversity under tillage practices:research progress[J]. Chinese Agricultural Science Bulletin, 2019,35(23):51-57.
[41]	Sheng Y, Zhu L. Biochar alters microbial community and carbon sequestration potential across different soil pH[J]. Science of the Total Environment, 2018,622-623:1391-1399.
[42]	王媛媛.磷酸盐对水稻土中异化铁还原菌丰度和群落结构的影响[D]. 咸阳:西北农林科技大学, 2018. Wang Y Y. Effect of phosphate on abundance and community structure of dissimilatory iron-reducing in paddy soil[D]. Xianyang:Northwest Agriculture and Forestry University, 2018.
[43]	陈蕾,张洪霞,李莹,等.微生物在地球化学铁循环过程中的作用[J]. 中国科学:生命科学, 2016,46(9):1069-1078. Chen L, Zhang H X, Li Y, et al. The role of microorganisms in the geochemical iron cycle[J]. Scientia Sinica Vitae, 2016,46(9):1069-1078.
[44]	Glaser B, Lehmann J, Zech W, et al. Ameliorating physical and chemical properties of highly weathered soils in the tropics with charcoal-a review[J]. Biology and Fertility of Soils, 2002,35(4):219-230.
[45]	张伟明.生物炭的理化性质及其在作物生产上的应用[D]. 沈阳:沈阳农业大学, 2012. Zhang W M. Physical and chemical properties of biochar and its application in crop production[D]. Shenyang:Shenyang Agricultural University, 2012.
[46]	Ali M A, Lee C H, Kim P J, et al. Effect of silicate fertilizer on reducing methane emission during rice cultivation[J]. Biology and Fertility of Soils, 2008,44(4):597-604.
[47]	林颖.水稻根际土壤铁还原微生物的丰度及多样性变化特征[D]. 杨凌:西北农林科技大学, 2019. Lin Y. Changing characteristics of iron-reducing microbial abundance and diversity in paddy rhizosphere soil[D]. Yangling:Northwest Agriculture and Forestry University, 2019.