Abstract：To further clarify the relationship between methanotrophs and its environment in landfill cover soils, the impact of environmental differences caused by different substrate concentrations and biochar groups on microorganisms were investigated by comparing the two important microbial activities, including methane oxidation and EPS generation. Substrate concentrations had the greatest impact on microbial diversity and population differences. With sufficient carbon source and oxygen, the amount of methane oxidation was mainly contributed by type I methanotrophs; otherwise, type Ⅱ methanotrophs dominated. Under high methane and low oxygen conditions, adding iron-rich biochar may effectively alleviate EPS blockage and facilitate the growth of Methylocystis.
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