Effect of quorum sensing in the formation of aerobic granular sludge
ZHI Li-ling1, MA Xin-xin1, LIU Qi-Xin1, WANG Shuo1,2,3, LI Ji1,2,3
1. School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, China; 2. Jiangsu Key Laboratory of Anaerobic & shy;Biotechnology, Wuxi 214122, China; 3. Water Treatment Technology and Technology in Jiangsu Province Materials Collaborative Innovation Center, Suzhou 215009, China
Abstract：By observing the variations of EPS, hydrolytic amino acids and acyl-homoserine lactones (AHLs) in the formation of aerobic granular sludge (AGS), the effect of quorum sensing is discovered in depth. The experimental results showed that in the granulation process, the final average size of AGS was approximately 150μm and the protein in EPS increased significantly, and the tightly bound EPS (TB-EPS) played an important role for granulation. The formation of aromatic substances and tyrosine protein substances in EPS provides material basis for AGS formation, and hydrophobic substances alanine and methionine promotes the formation of AGS and maintains the stability. Quorum sensing was generally strengthened during the formation process of AGS. The concentration of C4-HSL decreased significantly, while C6-HSL, 3OC6-HSL and C8-HSL were correlated with EPS, PN and PS, furthermore, C6-HSL and C8-HSL were presented significantly positively correlated with proteins and polysaccharides in TB-EPS.
支丽玲, 马鑫欣, 刘奇欣, 王硕, 李激. 好氧颗粒污泥形成过程中群感效应的作用研究[J]. 中国环境科学, 2020, 40(5): 2148-2156.
ZHI Li-ling, MA Xin-xin, LIU Qi-Xin, WANG Shuo, LI Ji. Effect of quorum sensing in the formation of aerobic granular sludge. CHINA ENVIRONMENTAL SCIENCECE, 2020, 40(5): 2148-2156.
苏海佳,王陆玺,邓爽,等. AGS技术及研究进展[J]. 化工进展, 2016,35(6):1914-1922. Su HJ, Wang LX, Deng S, et al. AGS technology and research progress[J]. Chemical Industry and Engineering Progress, 2016,35(6):1914-1922.
[2]
王明阳,曹素兰,王孙艳,等. AGS的工程应用及其研究进展[J]. 水处理技术, 2018,44(11):11-18. Wang M Y, Cao S L, Wang S Y, et al. Engineering application of AGS and its research progress[J]. Water Treatment Technology, 2018, 44(11):11-18.
[3]
卢鹏程.胞外聚合物对污泥性质的影响[J]. 中国化工贸易, 2017, 9(4):210-223. Lu PC. Effects of extracellular polymers on sludge properties[J]. China Chemical Industry Trade, 2017,9(4):210-223.
[4]
Wang Z, Zhu L, Xu X Y, et al. Understanding of aerobic sludge granulation enhanced by sludge retention time in the aspect of quorum sensing[J]. Bioresource Technology:Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies, 2019:226-234.
[5]
Bernet N, Moletta R, Dangcong P, et al. Aerobic granular sludge-a case report[J]. Water Research:A Journal of the International Water Association, 1999,33(3):890-893.
[6]
Melanie P, Jacobsen I. Quorum sensing by farnesol revisited[J]. Current Genetics:Eukaryotes with Emphasis on Yeasts, Fungi, Mitochondria, Plastids, 2017,63(5):791-797.
[7]
Zhu Y L, Hou H M, Zhang G L, et al. AHLs regulate biofilm formation and swimming motility of Hafnia alvei H4[J]. Front Microbiol., 2019:1013-1130.
[8]
Chen H, Li A, Cui D, et al. N-Acyl-homoserine lactones and autoinducer-2-mediated quorum sensing during wastewater treatment[J]. Applied Microbiology and Biotechnology, 2017,102(3):1119-1130.
[9]
Valle A, Bailey M J, Whiteley A S, et al. N-acyl-L-homoserine lactones (AHLs) affect microbial community composition and function in activated sludge[J]. Environmental Microbiology, 2004, 6(4):424-433.
[10]
王硕.低温AGS形成过程及其特性研究[D]. 黑龙江:哈尔滨工业大学, 2013. Wang S. Study on the formation process and characteristics of low-temperature AGS[D]. Heilongjiang:Harbin Institute of Technology, 2013.
[11]
王杰,彭永臻,杨雄,等.不同碳源种类对AGS合成PHA的影响[J]. 中国环境科学, 2015,35(8):2360-2366. Wang J, Peng Y Z, Yang X, et al. Effects of different carbon source types on the synthesis of PHA by AGS[J]. China Environmental Science, 2015,35(8):2360-2366.
[12]
国家环保总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M]. 第4版.北京:中国环境科学出版社, 2002:123-200. Editorial Board of the State Environmental Protection Administration "Analysis Method for Water and Wastewater Monitoring". Water and wastewater monitoring and analysis method[M]. 4th Edition. Beijing:China Environmental Science Press, 2002:123-200.
[13]
Jia SY, Zhu W, Zhang XX, et al. Metagenomic analysis of cadmium and copper resistance genes in activated sludge of a tannery wastewater treatment plant[J]. Journal of Environmental Biology, 2013,34(2Spec No):375-380.
[14]
唐堂,王硕,王玉莹,等.SBR不同沉降时间的污泥特性研究[J]. 中国给水排水, 2018,(3):85-90. Tang T, Wang S, Wang YY, et al. Study on the sludge characteristics of SBR with different settling time[J]. China Water & Wastewater, 2018,(3):85-90.
[15]
Liang Z, Li W, Yang S, et al. Extraction and structural characteristicsof extracellular polymeric substances (EPS), pelletsin autotrophic nitrifying biofilm and activated sludge[J]. Chemosphere, 2010,81(5):626-632.
[16]
李爽,陈启,蔡明明,等.液相色谱法与氨基酸分析仪法测定人乳中水解氨基酸的比较研究[J]. 食品安全质量检测学报, 2014, 5(7):2073-2079. Li S, Chen Q, Cai M M, et al. Comparative study on determination of hydrolyzed amino acids in human milk by liquid chromatography and amino acid analyzer[J]. Journal of Food Safety and Quality Testing, 2014,5(7):2073-2079.
[17]
Wang H, Song Q, Wang J, et al. Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sludge sequencing batch reactor with high dissolved oxygen:Effects of carbon to nitrogen ratios[J]. Science Total Environment, 2018,64(2):1145-1152.
[18]
Jiang B, Liu Y. Roles of ATP-dependent N-acylhomoserine lactones (AHLs) and extracellular polymeric substances (EPSs) in aerobic granulation[J]. Chemosphere, 2012,88(9):1058-1064.
[19]
Wang J, Ding L, Li K, et al. Development of an extraction method and LC-MS analysis for N-acylated-l-homoserine lactones (AHLs) in wastewater treatment biofilms[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2017,1041-1042:37-44.
[20]
侯爱月,李军,王昌稳,等.不同好氧颗粒污泥中微生物群落结构特点[J]. 中国环境科学, 2016,36(4):1136-1144.金正宇,郗皓,苑泉,等.进水模式对强化脱氮好氧颗粒污泥培养的影响[J]. 中国环境科学, 2018,(3):935-942. Hou AY, Li J, Wang CW, et al. Characteristics of microbial community structure in different aerobic granular sludge[J]. China Environmental Science, 2016,36(4):1136-1144;Jin Zhengyu, Xi Hao, Yuan Quan, et al. Effect of water inflow mode on enhanced cultivation of denitrification aerobic granular sludge[J]. China Environmental Science, 2018,(3):935-942.
[21]
彭昌盛,张倩,徐兴永,等.团聚-分散行为对悬浮液Zeta电位的影响[J]. 中国海洋大学学报(自然科学版), 2010,40(10):121-126. Peng C S, Zhang Q, Xu X Y, et al. Effect of agglomeration-dispersion behavior on the Zeta potential of suspensions[J]. Journal of Ocean University of China (Natural Science), 2010,40(10):121-126.
[22]
徐明进,李明远,彭勃,等.Zeta电位和界面膜强度对水包油乳状液稳定性影响[J]. 应用化学, 2007,(6):623-627. Xu M J, Li M Y, Peng Bo, et al. Effects of Zeta potential and interface membrane strength on the stability of oil-in-water emulsions[J]. Chinese Journal of Applied Chemistry, 2007,(6):623-627.
[23]
ZhangD, Pan X, Liu J. Binding of phenanthrene to extracellular polymeric substances (EPS) from aerobic activated sludge:A fluorescence study[J]. Colloids and Surfaces, B. Biointerfaces, 2010, 80(1):103-106.
[24]
Su H J, Deng S, Wang L X. Role and influence of extracellular polymeric substances on the preparation of aerobic granular sludge[J]. Journal of Environmental Management, 2016,173(11):49-54.
[25]
郭莉娜.藻类生物膜优选及脱氮除磷实验研究[D]. 广西壮族自治区:广西大学, 2014. Guo L N. Algae biofilm optimization and experimental research on nitrogen and phosphorus removal[D]. Guangxi Zhuang Autonomous Region:Guangxi University, 2014.
[26]
朱大伟,武道吉,孙翠珍,等.三维荧光光谱(3D-EEM)技术在溶解性有机质(DOM)分析中的应用[J]. 净水技术, 2015,34(1):14-17. Zhu D W, Wu D J, Sun C Z, et al. Application of three-dimensional fluorescence spectroscopy (3D-EEM) technology in the analysis of dissolved organic matter (DOM)[J]. Water Purification Technology, 2015,34(1):14-17.
[27]
邵尤炼.胞外多聚物在厌氧污泥颗粒化及应用过程中的作用研究[D]. 江苏:江南大学, 2014. Shao Y L. Study on the role of extracellular polymers in the granulation and application of anaerobic sludge[D]. Jiangsu:Jiangnan University, 2014.
[28]
郝晓地,周鹏,曹亚莉.污水处理中腐殖质的来源及其演变过程[J]. 环境工程学报, 2017,11(1):1-11. Hao X D, Zhou P, Cao Y L. Sources and evolution of humus in wastewater treatment[J]. Journal of Environmental Engineering, 2017,11(1):1-11.
[29]
焦扬,欧阳慧灵,何伟,等.富里酸存在条件下铜对普通小球藻光合作用与细胞生理的影响[J]. 环境科学学报, 2017,37(8):3185-3193. Jiao Y, Ouyang H L, He W, et al. Effects of copper on photosynthesis and cell physiology of Chlorella vulgaris in the presence of fulvic acid[J]. Journal of Environmental Science, 2017,37(8):3185-3193.
[30]
Zhang S H, Zeng X F, Ren M, et al. Novel metabolic and physiological functions of branched chain amino acids:a review[J]. Journal of Animal Science and Biotechnology, 2017,8(3):501-512.
[31]
王义富.右旋氨基酸对废水处理反应器中微生物聚集体的作用机制[D]. 山东:山东大学, 2014. Wang Y F. Mechanism of D-amino acid on microbial aggregates in wastewater treatment reactor[D]. Shandong:Shandong University, 2014.
[32]
Kohara A K K. Amino acid sensing using an ion-sensitive field-effect transistor[J]. Chemistry and chemical industry, 2012,6(5):397-400.
[33]
M Zhao, Li W, Liu K, et al. C4-HSL aptamers for blocking qurom sensing and inhibiting biofilm formation in Pseudomonas aeruginosa and its structure prediction and analysis[J]. PLoS One, 2019,14(2).
[34]
Ahumedo M M, Galindo J F, Vergara L J, et al. The role of LasR active site amino acids in the interaction with the acyl homoserine lactones (AHLs) analogues:A computational study[J]. J Mol Graph Model, 2019,86:113-124.
[35]
李旖瑜,郑平,张萌.颗粒污泥结构体及其粘连机理[J]. 中国给水排水, 2017,33(22):33-37. Li Y Y, Zheng P, Zhang M. Granular sludge structure and its adhesion mechanism[J]. China Water & Wastewater, 2017,33(22):33-37.
[36]
Morohoshi T, Kamimura Y, Sato N, et al. Distribution and characterization of N-acylhomoserine lactone (AHL)-degrading activity and AHL lactonase gene (qsdS) in Sphingopyxis[J]. J Biosci Bioeng, 2019,127(4):411-417.
