Abstract：The strain JQ1004 with capability of heterotrophic nitrification were able to grow and metabolism under different initial ammonium concentrations in range of 0~2000mg/L.However,the strain were completely inhibited in concentration of 2500mg/L.When strain JQ1004 was cultivated at 30℃,pH 7.5,160rpm with the initial ammonium of 100,300,500,700,1000,1500,2000mg/L,the maximum specific growth rates were 0.251,0.308,0.286,0.243,0.197,0.115,0.088h-1,and the corresponding ammonium specific removal rates reached 1.335,1.906,1.859,1.759,1.562,1.286,0.965g/g (DCW·d),respectively.Due to the inhibition of free ammonia and high-strength concentration of ammonium,the specific growth rate and degradation rate of ammonia increased at first and decreased with the increase of initial concentration of ammonia nitrogen (free ammonia).Three kinetic models (Haldane,Yano,Aiba) were fitted well to the experimental growth kinetic data with the correlation coefficients (R2) of 0.9944,0.9983 and 0.9929.For Haldane model,the values of μmax,Ks,and Ki were 2.604h-1,22.57mg/L,and 1445.31mg/L,respectively.The large values of Ki,far greater than that of autotrophic nitrifiers or anaerobic ammonium-oxidizing bacteria,indicated that JQ1004 had good tolerance against high ammonium concentrations.Besides,the specific growth rate reached a maximum value of 0.583h-1 when the concentration of free ammonia was 5.436mg/L.These results indicated possible future applications of JQ1004 in removing nitrogen and organic carbon from high-strength ammonium wastewater.
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