低温下AHLs对多级A/O工艺中好氧生物膜特性的影响

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摘要针对低温条件下多级A/O工艺出水氨氮容易超标问题，进行外源添加酰基高丝氨酸内酯类信号分子（AHLs）对低温下多级A/O工艺好氧段生物膜特性的影响研究.结果表明:添加100nmol/L的己酰基高丝氨酸内酯（C6-HSL）后生物膜硝化效率提高了22.34%，十二烷酰基高丝氨酸内酯（C12-HSL）则将生物附着量提高了24.47%，且C6-HSL和C12-HSL对生物膜硝化效率和生物附着量的提高作用均具有长期性.这是因为C6-HSL能够将低温下好氧段生物膜硝化活性提高17.56%，而C12-HSL可以将胞外聚合物（EPS）中蛋白质（PN）的含量提高67.37%.此外，C6-HSL和C12-HSL均能促进对应信号分子的内源释放，因此对生物膜硝化活性和附着性能具有长期积极影响.高通量测序分析表明C6-HSL可提高Nitrosomonas（亚硝化单胞菌）的丰度，故能提高R2中生物膜硝化活性;而外源添加C12-HSL对Rhodobacter（红杆菌属）丰度影响较大，这是实验组R5生物膜EPS中PN含量增加的重要原因.

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	韩浩

关键词 ：多级A/O, 生物膜, 低温, 群体感应

Abstract：Aiming at the problem that the ammonia nitrogen in the effluent of the multi-stage A/O technology is likely to exceed the standard under low temperature conditions, the effect of exogenous addition of acyl homoserine lactone signal molecules (AHLs) on the characteristics of the biofilm in the aerobic zone of the multi-stage A/O technology at low temperature was studied. Results showed that the addition of 100nmol/L hexanoyl homoserine lactone (C6-HSL) increased the biofilm nitrification efficiency by 22.34%, and lauryl homoserine lactone (C12-HSL) increased the amount of attachment biomass by 24.47%, both C6-HSL and C12-HSL had long-term effects on the nitrification efficiency and biological adhesion of biofilm. This was because C6-HSL can increase the aerobic biofilm nitrification activity by 17.56% at low temperature, while C12-HSL could increase the content of protein (PN) in EPS by 67.37%. In addition, both C6-HSL and C12-HSL could promote the endogenous release of the same signal, thus having a long-term positive impact on the nitrification activity and adhesion performance of biofilm. The high-throughput sequencing analysis showed that C6-HSL can increase the abundance of Nitrosomonas, and thus enhancing the nitrification activity of biofilm in R2; while the addition of C12-HSL had a greater impact on the abundance of Rhodobacter, which was an important reason for the increase of PN content in EPS of R5 biofilm.

Key words： multi-stage A/O technology biofilm low temperature quorum sensing

收稿日期: 2020-09-08

PACS:

X703

基金资助:水体污染控制与治理科技重大专项（2018ZX07701001-25）

通讯作者: 李军,教授,jglijun@bjut.edu.cn E-mail: jglijun@bjut.edu.cn

作者简介: 周荣煊(1995-),男,四川雅安人,北京工业大学硕士研究生,主要从事分段进水多级A/O工艺研究.

引用本文:

周荣煊, 马潇然, 李军, 张林华, 张晶, 张亚超, 韩浩. 低温下AHLs对多级A/O工艺中好氧生物膜特性的影响[J]. 中国环境科学, 2021, 41(5): 2133-2140. ZHOU Rong-xuan, MA Xiao-ran, LI Jun, ZHANG Lin-Hua, ZHANG Jing, ZHANG Ya-chao, HAN Hao. Effects of AHLs at low temperature on the characteristics of mature biofilms in aerobic zone of multi-stage A/O technology. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(5): 2133-2140.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I5/2133

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