Effects of different microplastics occurrence environment on seed germination and seedling growth of wheat (Triticum aestivum L.)
ZHANG Yan1,2,3, DOU Ming1,4, ZOU Lei5, LI Ping2,6, LIANG Zhi-jie3,6, LI Gui-qiu4
1. School of Water Conservancy Engineering, Zhengzhou University, Zhengzhou 450001, China; 2. Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China; 3. Laboratory of Quality and Safety Risk Assessment for Agro-Products on Water Environmental Factors, Ministry of Agriculture, Xinxiang 453002, China; 4. School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China; 5. Key Laboratory of Water Cycle and Related Land Surface Processes, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; 6. Agricultural Water Soil Environmental Field Research Station of Xinxiang, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China
Abstract：In order to reveal the effects of different microplastics occurrence environment on seed germination and seedling growth of wheat (Triticum aestivum L.), the Polypropylene (PP), High Density Polyethylene (HDPE) and Biodegradable Polylactic Acid (PLA) were selected, the pot experiments were carried out in natural environment with the particle sizes (150μm, 1000μm and 4000μm) and mass concentrations (0.1g/kg, 0.5g/kg and 1g/kg), and the germination rates, germination inhibition rates, growth characteristics and seedling growth trends of wheat were analyzed by statistical analysis and variance analysis. The results showed that the average germination level of wheat seeds was reduced under different microplastics exposure, and the average germination inhibition rates of wheat seeds showed HDPE>PLA>PP; the average germination inhibition rates of wheat seeds under medium particle size and medium mass concentration of microplastics were the highest, which were 5.65% and 4.55%, respectively, while the inhibitory effects of microplastics on wheat seed germination were weakened at low or high particle size and mass concentration under low or high particle size and mass concentration of microplastics; the particle size of microplastics had a greater effect on the germination rate of wheat seeds, followed by the type and mass concentration of microplastics. The effects of microplastics HDPE and PLA on the growth characteristics of wheat seeds (germination potential, germination index, vigor index and average germination time) were more significant than those of microplastics PP compared with the control group, and the vigor index of wheat seeds was most significantly affected by the type, particle size and mass concentration of microplastics. The average growth rates of the control group and different types of microplastics on wheat seedlings showed CK>PP>HDPE>PLA, while the inhibitory effects of different microplastics on the growth of wheat seedlings showed PLA>HDPE>PP, and the type of microplastics had the most significant effect on the growth of wheat seedlings.
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