厌氧氨氧化UASB系统对氨氮的超量去除机制研究

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摘要采用UASB反应器在改变NO₂^--N/NH₄⁺-N比条件下，考察厌氧氨氧化系统对NH₄⁺-N的超量去除特征、相关酶的催化活性以及污泥菌群结构.结果表明，随着进水NO₂^--N浓度降低，反应器对NH₄⁺-N的去除量相比理论较大，在停供NO₂^--N情况下，反应器内NH₄⁺-N去除可达55 mg/L.反应器内NH₄⁺-N的去除并不是是来自进水中SO₄^2-和Fe³⁺/EDTA络合物，而是存在NH₄⁺-N的好氧硝化.过氧化氢酶测定联合分子生物学技术分析显示，好氧硝化的所需氧量分别来自进水和过氧化氢酶产氧.反应器底部污泥层的氨氧化菌（AOB）、厌氧氨氧化菌（AnAOB）活性优于上部污泥层，相反，上部污泥层的异养反硝化菌（HDB）活性优于底部污泥层，二者协同将NH₄⁺-N转化为N₂.

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	袁林杰
	袁林江
	陈希
	杨睿
	于丽萍
	王刚
	贺向峰
	杨江伟

关键词 ：厌氧氨氧化, 氮超量去除, 过氧化氢, 过氧化氢酶

Abstract：The UASB reactor was used to investigate the excess removal characteristics of NH₄⁺-N by the anaerobic ammonia oxidation system, the catalytic activity of related enzymes, and the bacterial community structure of the sludge with changing ratio of NO₂^--N/NH₄⁺-N. The results showed that as the concentration of influent NO₂^--N decreaseed, the removal of NH₄⁺-N by the reactor increaseed compared to the theory. When the supply of nitrite was stopped, the removal of NH₄⁺-N in the reactor reached up to 55mg/L. The removal of NH₄⁺-N was not due to the anaerobic ammonia oxidization by the SO₄^2- and Fe³⁺/EDTA complex from the feed water, but the aerobic nitration of NH₄⁺-N. The analysis of catalase determination combined with molecular biology technology showed that the oxygen required for aerobic nitrification came from influent water and catalase oxygen production. The activity of ammonia oxidizing bacteria (AOB) and anaerobic ammonia oxidizing bacteria (AnAOB) in the bottom sludge bed was better than that in the upper sludge bed. On the contrary, the activity of heterotrophic denitrifying bacteria (HDB) in the upper sludge bed was better than that in the bottom sludge bed, and the two parts of sludge bed synergistically converted NH₄⁺-N into N₂.

Key words： anaerobic ammonia oxidation nitrogen excess removal hydrogen peroxide catalase

收稿日期: 2021-03-10

PACS:

X703

基金资助:国家自然科学基金资助项目（51878538）；陕西省自然科学基金资助项目（2021JQ-690）

通讯作者: 袁林江,教授,yuanlinjiang@xauat.edu.cn E-mail: yuanlinjiang@xauat.edu.cn

作者简介: 袁林杰(1995-),男,安徽合肥人,西安建筑科技大学硕士研究生,主要从事城市污水处理理论与技术.

引用本文:

袁林杰, 袁林江, 陈希, 杨睿, 于丽萍, 王刚, 贺向峰, 杨江伟. 厌氧氨氧化UASB系统对氨氮的超量去除机制研究[J]. 中国环境科学, 2021, 41(10): 4686-4694. YUAN Lin-jie, YUAN Lin-jiang, CHEN Xi, YANG Rui, YU Li-ping, WANG Gang, HE Xiang-feng, YANG Jiang-wei. Mechanism of excessive removal of ammonia nitrogen by anammox UASB system. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(10): 4686-4694.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I10/4686

[1]	Kartal B, Kuenen J G, Van Loosdrecht M C M. Sewage Treatment with Anammox[J]. Science, 2010,328(5979):702-703.
[2]	Ali M, Okabe S. Anammox-based technologies for nitrogen removal:Advances in process start-up and remaining issues[J]. Chemosphere, 2015,141(12):144-153.
[3]	Wu P, Zhang X, Wang X, et al. Characterization of the start-up of single and two-stage Anammox processes with real low-strength wastewater treatment[J]. Chemosphere, 2019,245.
[4]	Strous M, Heijnen J J, Kuenen J G, et al. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J]. Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[5]	Bae H, Park K S, Chung Y C, et al. Distribution of anammox bacteria in domestic WWTPs and their enrichments evaluated by real-time quantitative PCR[J]. Process Biochemistry, 2010,45(3):323-334.
[6]	Xiong L, Wang Y Y, Tang C J, et al. Start-up characteristics of a granule-based Anammox UASB reactor seeded with anaerobic granular sludge[J]. Biomed Res Int, 2013,2013:1-9.
[7]	Lotti T, Kleerebezem R, Lubello C, et al. Physiological and kinetic characterization of a suspended cell anammox culture[J]. Water Research, 2014,60:1-14.
[8]	闾刚,李田,徐乐中,等.基于不同接种污泥复合型厌氧氨氧化反应器的快速启动特征[J]. 环境科学, 2017,38(10):4324-4331.Lu G, Li T, Xu LZ, et al. Quick start-up characteristics of a composite anammox reactor based on different inoculated sludge[J]. Environmental Science, 2017,38(10):4324-4331.
[9]	李祥,林兴,王凡,等.厌氧氨氧化污泥中氨氧化的潜在电子受体[J]. 环境科学, 2017,38(7):2941-2946.Li X, Lin X, Wang F, et al. Potential electron acceptors for ammonia oxidation in anammox sludge[J]. Environmental Science, 2017,38(7):2941-2946.
[10]	Sabumon P C. Anaerobic ammonia removal in presence of organic matter:A novel route[J]. Journal of Hazardous Materials, 2007,149(1):49-59.
[11]	张蕾,郑平,何玉辉,等.硫酸盐型厌氧氨氧化性能的研究[J]. 中国科学, 2008,(12):1113-1119.Zhang L, Zheng P, He YH, et al. Study on the performance of sulfate-type anammox[J]. Chinese Science, 2008,(12):1113-1119.
[12]	国家环境保护总局.水和废水监测分析方法[M]. 北京:中国环境科学出版社, 2004.State Environmental Protection Administration. Water and wastewater monitoring and analysis methods[M]. Beijing:China Environmental Science Press, 2004.
[13]	李亚琪,王弘恺,朱维晃,等.聚苯胺/蒽醌修饰碳毡作为阴极在电芬顿中的应用[J]. 环境科学学报, 2020,40(11):3905-3912.Li Y Q, Wang H K, Zhu W H, et al. Application of polyaniline/anthraquinone modified carbon felt as a cathode in electro-Fenton[J]. Acta Scientiae Circumstantiae, 2020,40(11):3905-3912.
[14]	赵良杰,彭党聪,吕恺,等.一段式部分亚硝化-厌氧氨氧化工艺处理中低浓度模拟氨氮废水[J]. 环境工程学报, 2020,15(1):143-151.Zhao L J, Peng D C, Lyu K, et al. Treatment of simulated medium ammonia wastewater by single-stage partial nitritation-anammox process[J]. Journal of Environmental Engineering, 2020,15(1):143-151.
[15]	郑照明,刘常敬,郑林雪,等.不同粒径的厌氧氨氧化颗粒污泥脱氮性能研究[J]. 中国环境科学, 2014,34(12):3078-3085.Zheng G M, Liu C J, Zheng L X, et al. Study on the denitrification performance of anaerobic ammonia oxidation granular sludge with different particle sizes[J]. China Environmental Science, 2014,34(12):3078-3085.
[16]	崔璟宜.活性污泥中过氧化氢酶活性的测定及影响因素分析[D]:吉林:吉林建筑大学, 2013.Cui JY. Determination of catalase activity in activated sludge and analysis of influencing factors[D]. Jilin:Jilin Jianzhu University, 2013.
[17]	Amann R I, Krumholz L R, Stahl D A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology[J]. Journal of Bacteriology, 1990,172(2):762-770.
[18]	Amann R I, Binder B J, Olson R J, et al. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations[J]. Appl. Environ. Microbiol., 1990,56(6):1919-1925.
[19]	Daims H, Br Hl A, Amann R, et al. The domain-specific probe EUB338is insufficient for the detection of all Bacteria:development and evaluation of a more comprehensive probe set[J]. Systematic & Applied Microbiology, 1999,22(3):434-444.
[20]	Kartal B, Rattray J, Niftrik L, et al. Candidatus "Anammoxoglobus propionicus" a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and applied microbiology, 2007,30(1):39-49.
[21]	Mobarry B K, Wagner M, Urbain V, et al. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria[J]. Applied & Environmental Microbiology, 1997,63(2):2156-2162.
[22]	Liu S, Yang F, Gong Z, et al. Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal[J]. Bioresource Technology, 2008,99(15):6817-6825.
[23]	Sawayama S. Possibility of anoxic ferric ammonium oxidation[J]. Journal of bioscience and bioengineering, 2006,101(1):70-72.
[24]	Kartal B, Rattray J, Niftrik L A V, et al. Candidatus "Anammoxoglobus propionicus" a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and applied microbiology, 2007,30(1):39-49.
[25]	Hulth S, Aller R C, Gilbert F. Coupled anoxic nitrification/manganese reduction in marine sediments[J]. Geochimicaet Cosmochimica Acta, 1999,63(1):49-66.
[26]	Zhang D, Cui L, Zhu H, et al. Treatment performance and microbial community under ammonium sulphate wastewater in a sulphate reducing ammonium oxidation process[J]. Environmental technology, 2020.
[27]	Fdz-Polanco F, Fdz-Polanco M, Fernandez N, et al. New process for simultaneous removal of nitrogen and sulphur under anaerobic conditions[J]. Water Research, 2001,35(4):1111-1114.
[28]	Sabumo P C. Effect of potential electron acceptors on anoxic ammonia oxidation in the presence of organic carbon[J]. Journal of hazardous materials, 2009,172(1):280-288.
[29]	Fu, Huihui, Gao, et al. Microbial oxidative stress response:Novel insights from environmental facultative anaerobic bacteria[J]. Archives of Biochemistry & Biophysics, 2015,584:28-35.
[30]	Blokhina O, Virolainen E, Fagerstedt K V. Antioxidants, oxidative damage and oxygen deprivation stress:A review[J]. Annals of Botany, 2003,91(2):179-194.
[31]	Davydova M N, Sabirova R Z. Anti-oxidant defense of the cell Desulfovibrio desulfuricans B-1388[J]. Anaerobe, 2003,9(1):39-41.
[32]	G D S W, I P, Y L M, et al. Purification and characterization of an iron superoxide dismutase and a catalase from the sulfate-reducing bacterium Desulfovibrio gigas[J]. Journal of bacteriology, 2000, 182(3):796-804.
[33]	A B, A N, M S, et al. Protection of Methanosarcina barkeri against oxidative stress:identification and characterization of an iron superoxide dismutase[J]. Archives of microbiology, 2000,174(3):213-216.
[34]	M L, M F, D T, et al. Reaction of the desulfoferrodoxin from Desulfoarculus baarsii with superoxide anion. Evidence for a superoxide reductase activity[J]. The Journal of biological chemistry, 2000,275(1):115-121.
[35]	L L H, V S N, O S A, et al. Rubrerythrin and rubredoxin oxidoreductase in Desulfovibrio vulgaris:A novel oxidative stress protection system[J]. Journal of bacteriology, 2001,183(1):101-108.
[36]	Anjali G, Sabumon P C. Development of simultaneous partial nitrification, anammox and denitrification (SNAD) in a non-aerated SBR[J]. International Biodeterioration & Biodegradation, 2017,119.
[37]	Jetten M S M, Logemann S, Muyzer G, et al. Novel principles in the microbial conversion of nitrogen compounds[J]. Antonie van Leeuwenhoek, 1997,71(1/2).
[38]	Guisasola A, Jubany I, Baeza J A, et al. Respirometric estimation of the oxygen affinity constants for biological ammonium and nitrite oxidation[J]. Journal of Chemical Technology & Biotechnology Biotechnology, 2010,80(4):388-396.
[39]	Liu X, Kim M, Nakhla G. Operational conditions for successful partial nitrification in a sequencing batch reactor (SBR) based on process kinetics[J]. Environmental technology, 2016,38(6):694-704.
[40]	Wang J, Lei Z, Wang L, et al. Insight into using up-flow anaerobic sludge blanket-anammox to remove nitrogen from an anaerobic membrane reactor during mainstream wastewater treatment[J]. Bioresource Technology, 2020,314.
[41]	Yue H, Zhang Y, He Y, et al. Keystone taxa regulate microbial assemblage patterns and functional traits of different microbial aggregates in simultaneous anammox and denitrification (SAD) systems[J]. Bioresource Technology, 2019,290.
[42]	Graaf A A, Van De, Mulder A, Bruijn P, De, et al. Anaerobic oxidation of ammonium is a biologically mediated process[J]. Applied & Environmental Microbiology, 1995,61(4):1246-1251.
[43]	付昆明,苏雪莹,王会芳,等.内回流对厌氧氨氧化UASB反应器脱氮性能的影响[J]. 中国环境科学, 2016,36(12):3560-3566.Fu K M, Su X Y, Wang H F, et al. Influence of internal reflux on the denitrification performance of UASB reactor[J]. China Environmental Science, 2016,36(12):3560-3566.
[44]	Miao Y, Peng Y, Zhang L, et al. Partial nitrification-anammox (PNA) treating sewage with intermittent aeration mode:Effect of influent C/N ratios[J]. Chemical Engineering Journal, 2018,334:664-672.
[45]	Lu H-F, Zheng P, Ji Q-X, et al. The structure, density and settlability of anammox granular sludge in high-rate reactors[J]. Bioresource Technology, 2012, 123(123):312-317.
[46]	许冬冬,康达,郭磊艳,等.厌氧氨氧化颗粒污泥研究进展[J]. 微生物学通报, 2019,46(8):1988-1997.Xu D D, Kang D, Guo Ly, et al. Research progress of anammox granular sludge[J]. Microbiology Bulletin, 2019,46(8):1988-1997.