Performance recovery of SPNA micro-granular sludge system after long-term starvation
HE Li-jin1,2, WANG Shao-po1,2, BI Yan-meng1,2, LI Jian-yu1,2, QIU Chun-sheng1,2, WANG Dong1,2, ZHENG Sheng-da3, YU Jing-jie1,2
1. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China; 2. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China; 3. Tianjin Sanbo Water Science and Technology Company Limited, Tianjin 300384, China
Abstract：In order to determine the performance recovery of single-stage partial nitritation anammox (SPNA) process after long-term starvation, a continuous flow reactor was used in this study, to investigate the feasibility of the recovery strategy, nitrogen removal performance and microbial community structure variations of SPNA system undergoing 161d starvation at room temperature (11~23℃). By controling DO concentration and influent ammonia nitrogen load, the inhibition and elimination of nitrite oxidizing bacteria (NOB) and the recovery and enrichment of ammonium oxidation bacteria(AOB) and anaerobic ammonium oxidation bacteria (AnAOB) were gradually realized. Within 68 days, the removal efficiency of total nitrogen and ammonia nitrogen increased to 72.13% and 94.75% respectively. The proportion of micro-granular sludge (3200μm) increased from 42.04% to 60.98%. The analysis of microbial community showed that the relative abundance of Candidatus Kuenenia increased to 25.53% after 161 days starvation, which showed that Candidatus Kuenenia has a stronger hunger tolerance. After the system recovery, the relative abundance of Candidatus Kuenenia gradually decreased to the level before the reactor starvation. The higher substrate utilization ability of AOB is the premise of system recovery, and the improvement of AnAOB activity is essential for system recovery. The successful recovery of the system performance showed that the starvation period of 161d at room temperature has reversible effect on the system, and it is feasible to store SPNA sludge at ambient temperature for a long time.
何丽金, 王少坡, 毕艳孟, 李剑宇, 邱春生, 王栋, 郑胜达, 于静洁. 长期饥饿后SPNA微颗粒污泥系统性能恢复[J]. 中国环境科学, 2021, 41(12): 5646-5653.
HE Li-jin, WANG Shao-po, BI Yan-meng, LI Jian-yu, QIU Chun-sheng, WANG Dong, ZHENG Sheng-da, YU Jing-jie. Performance recovery of SPNA micro-granular sludge system after long-term starvation. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(12): 5646-5653.
Pedrouso A, Aiartza I, Morales N, et al. Pilot-scale ELAN® process applied to treat primary settled urban wastewater at low temperature via partial nitritation-anammox processes[J]. Separation and Purification Technology, 2018,200:94-101.
Yue X, Yu G, Liu Z, et al. Fast start-up of the CANON process with a SABF and the effects of pH and temperature on nitrogen removal and microbial activity[J]. Bioresource Technology, 2018,254:157-165.
Wang S, Li J, Wang D, et al. Start-up of single-stage partial nitritation-anammox micro-granules system: Performance and microbial community dynamics[J]. Environmental Research, 2020,186:109581.
Cao Y, van Loosdrecht M C M, Daigger G T. Mainstream partial nitritation-anammox in municipal wastewater treatment: status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017,101(4):1365-1383.
Xing B S, Guo Q, Jiang X Y, et al. Long-term starvation and subsequent reactivation of anaerobic ammonium oxidation (anammox) granules[J]. Chemical Engineering Journal, 2016,287:575-584.
Leitão R C, Van Haandel A C, Zeeman G, et al. The effects of operational and environmental variations on anaerobic wastewater treatment systems: A review[J]. Bioresource Technology, 2006,97(9): 1105-1118.
Xu D, Kang D, Ding A, et al. Response of FANIR system to starvation stress:“Dormancy”[J]. Water Research, 2020,171:115380.
Ye L, Li D, Zhang J, et al. Resuscitation of starved anaerobic ammonium oxidation sludge system: Impacts of repeated short-term starvation[J]. Bioresource Technology, 2018,263:458-466.
Reeve P J, Mouilleron I, Chuang H P, et al. Effect of feed starvation on side-stream anammox activity and key microbial populations[J]. Journal of Environmental Management, 2016,171:121-127.
Li Y, Yu T, Kang D, et al. Sources of anammox granular sludge and their sustainability in treating low-strength wastewater[J]. Chemosphere, 2019,226:229-237.
Ganesan S, Vadivelu V M. Effect of storage conditions on maintaining anammox cell viability during starvation and recovery[J]. Bioresource Technology, 2019,296:122341.
Li J, Zhang L, Liu J, et al. Hydroxylamine addition and real-time aeration control in sewage nitritation system for reduced start-up period and improved process stability[J]. Bioresource Technology, 2019,294:122183.
Sui Q, Wang Y, Wang H, et al. Roles of hydroxylamine and hydrazine in the in-situ recovery of one-stage partial nitritation-anammox process: Characteristics and mechanisms[J]. Science of the Total Environment, 2020,707:135648.
李佳,李夕耀,张琼等.投加羟胺原位恢复城市污水短程硝化-厌氧氨氧化工艺[J]. 中国环境科学, 2019,39(7):2789-2795. Li J, Li X Y, Zhang Q et al. In-situ restoring demostic wastewater partial nitritation/anammox (PN/A) process by addition of hydroxylamine[J]. China Environmental Science, 2019,39(7):2789-2795.
Ali M, Okabe S. Anammox-based technologies for nitrogen removal: advances in process start-up and remaining issues[J]. Chemosphere, 2015,141:144-153.
Vázquez-Padín J R, Pozo M J, Jarpa M, et al. Treatment of anaerobic sludge digester effluents by the CANON process in an air pulsing SBR[J]. Journal of Hazardous Materials, 2009,166(1):336-341.
Qian F, Gebreyesus A T, Wang J, et al. Single-stage autotrophic nitrogen removal process at high loading rate: granular reactor performance, kinetics, and microbial characterization[J]. Applied microbiology and biotechnology, 2018,102(5):2379-2389.
Wiesmann U. Biological nitrogen removal from wastewater[J]. Biotechnics/Wastewater, 1994:113-154.
Wang S, Liu Y, Niu Q, et al. Nitrogen removal performance and loading capacity of a novel single-stage nitritation-anammox system with syntrophic micro-granules[J]. Bioresource Technology, 2017,236:119-128.
Chen G, Zhang Y, Wang X, et al. Optimizing of operation strategies of the single-stage partial nitrification-anammox process[J]. Journal of Cleaner Production, 2020,256:120667.
王朝朝,高鹏,闫立娜等.基于改良ASM1的SNAD工艺启动和优化研究[J]. 中国环境科学, 2021,41(8):3590-3600. Wang C C, Gao P, Yan L N, et al. Research on Start-up and Optimization of SNAD Process Based on Modified ASM1[J]. China Environmental Science, 2021,41(8):3590-3600.
Li J, Li J, Gao R, et al. A critical review of one-stage anammox processes for treating industrial wastewater: optimization strategies based on key functional microorganisms[J]. Bioresource Technology, 2018,265:498-505.
Tang C J, Zheng P, Wang C H, et al. Performance of high-loaded ANAMMOX UASB reactors containing granular sludge[J]. Water Research, 2011,45(1):135-144.
Qian Y, Ding Y, Ma H, et al. Startup and performance of a novel single-stage partial nitritation/anammox system for reject water treatment[J]. Bioresource Technology, 2021,321:124432.
Pedrouso A, Tocco G, del Río A V, et al. Digested blackwater treatment in a partial nitritation-anammox reactor under repeated starvation and reactivation periods[J]. Journal of Cleaner Production, 2020,244:118733.
韩朝丽.CANON工艺快速启动及自养脱氮污泥活性恢复研究[D]. 新乡:河南师范大学, 2018. Han C L. Rapid start-up of CANON process and activity recovery of autotrophic denitrification sludge[D]. Xinxiang: Henan Normal University, 2018.
Liu Y Q, Tay J H. Characteristics and stability of aerobic granules cultivated with different starvation time[J]. Applied Microbiology and Biotechnology, 2007,75(1):205-210.
Li H, Wang S, Zhao F, et al. Evaluating the effects of micro-zones of granular sludge on one-stage partial nitritation-anammox nitrogen removal[J]. Bioprocess and Biosystems Engineering, 2020,43(6):1037-1049.
Liu W, Yang D, Chen W, et al. High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures[J]. Bioresource Technology, 2017,231:45-52.
Chen H, Ma C, Yang G F, et al. Floatation of flocculent and granular sludge in a high-loaded anammox reactor[J]. Bioresource Technology, 2014,169:409-415.
Li B, Wang Y, Li X, et al. Comparing the nitrogen removal performance and microbial communities of flocs-granules hybrid and granule-based CANON systems[J]. Science of the Total Environment, 2020,703:134949.
Shi Y, Huang J, Zeng G, et al. Exploiting extracellular polymeric substances (EPS) controlling strategies for performance enhancement of biological wastewater treatments: an overview[J]. Chemosphere, 2017,180:396-411.
Iorhemen O T, Zaghloul M S, Hamza R A, et al. Long-term aerobic granular sludge stability through anaerobic slow feeding, fixed feast-famine period ratio, and fixed SRT[J]. Journal of Environmental Chemical Engineering, 2020,8(2):103681.
Rudnick P, Meletzus D, Green A, et al. Regulation of nitrogen fixation by ammonium in diazotrophic species of proteobacteria[J]. Soil Biology and Biochemistry, 1997,29(5/6):831-841.
Woebken D, Fuchs B M, Kuypers M M M, et al. Potential interactions of particle-associated anammox bacteria with bacterial and archaeal partners in the Namibian upwelling system[J]. Applied and Environmental Microbiology, 2007,73(14):4648-4657.
李云飞,王丝可,左剑恶.一体式厌氧氨氧化反应器常温运行与微生物群落研究[J]. 中国环境科学, 2021,41(3):1172-1180. Li Y F, Wang S K, Zuo J E. Research on the performance and microbial community of a one-stage partial nitritation-anammox reactor at room temperature[J]. China Environmental Science, 2021, 41(3):1172-1180.
Wang T, Zhang H, Yang F. Long-term storage and subsequent reactivation of Anammox sludge at 35℃[J]. Desalination and Water Treatment, 2016,57(52):24716-24723.
Zhu G, Wang S, Wang C, et al. Resuscitation of anammox bacteria after> 10,000years of dormancy[J]. The ISME Journal, 2019,13(4): 1098-1109.
Liu W, Yang Q, Ma B, et al. Rapid achievement of nitritation using aerobic starvation[J]. Environmental Science & Technology, 2017, 51(7):4001-4008.