镉对孕鼠胎盘激素合成功能的毒性效应

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摘要为探究孕期低剂量镉暴露对胎盘激素合成功能的毒性效应,选择雌三醇和孕酮激素作为研究对象,基于研究的孕期低剂量镉暴露小鼠模型,通过生物化学方法检测镉暴露小鼠的血液和胎盘中的雌三醇和孕酮激素含量及其合成底物浓度,并通过实时定量PCR法对胎盘中相关激素合成基因的表达水平进行检测.结果表明,孕期低剂量镉暴露可导致小鼠血清和胎盘中的孕酮激素含量在妊娠中末期分别降低43%和13%,但是对雌三醇激素含量则无显著性影响;镉暴露小鼠胎盘中孕酮合成底物的浓度无显著性变化;与对照组相比,镉暴露小鼠胎盘中孕酮合成酶HSD3B6及其表达调控基因D3的表达水平在妊娠中末期分别降低73%和85%.孕期低剂量镉暴露抑制了妊娠中末期胎盘中孕酮激素的合成,该抑制作用可能与胎盘中的孕酮合成酶HSD3B6及其表达调控基因D3的表达水平降低相关.

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	许鹏
	郭婧
	李子燕
	任焕
	靳雅伶
	王峰
	杜红丽
	黎治浪
	刘明
	李少钦
	赵美蓉
	王兰

关键词 ：孕期暴露, 胎盘, 雌三醇, 孕酮, 镉

Abstract：This study explored the toxic effects of low-dose maternal cadmium (Cd) exposure on the levels of estriol, progesterone and their substrates in the mouse serum and placentas. The mRNA levels of the genes involved in the hormone production were also examined by quantitative real-time PCR. The results showed that the concentration of progesterone, but not estriol, was significantly decreased by 43% and 13% respectively in the serum and placentas from the Cd-exposed mice, despite that the level of substrate for progesterone production remained unchanged in the placentas. The mRNA expression levels of progesterone synthase HSD3B6 gene and its regulatory gene D3 were significantly decreased by 73% and 85% in the Cd-exposed placentas respectively. In conclusion, low-dose maternal Cd exposure can inhibit the progesterone production in the placentas, which is likely to be due to the decrease in the expressions of hormone gene related to the hormone synthesis, such as HSD3B6 and D3.

Key words： maternal exposure placenta estriol progesterone cadmium

收稿日期: 2021-07-17

PACS:

X174

基金资助:国家自然科学基金（21806093）；中国博士后科学基金特别资助（2019T120342）；中国博士后科学基金面上资助（2017M621482）

通讯作者: 许鹏,副教授,xupeng0313@aliyun.com E-mail: xupeng0313@aliyun.com

作者简介: 许鹏(1985-),男,河北张家口人,副教授,博士,研究方向为生殖毒理学.发表论文20余篇.

引用本文:

许鹏, 郭婧, 李子燕, 任焕, 靳雅伶, 王峰, 杜红丽, 黎治浪, 刘明, 李少钦, 赵美蓉, 王兰. 镉对孕鼠胎盘激素合成功能的毒性效应[J]. 中国环境科学, 2022, 42(3): 1359-1368. XU Peng, GUO Jing, LI Zi-yan, REN Huan, JIN Ya-ling, WANG Feng, DU Hong-li, LI Zhi-lang, LIU Ming, LI Shao-qin, ZHAO Mei-rong, WANG Lan. The toxic effects of maternal cadmium exposure on the hormone production of the mouse placentas. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1359-1368.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I3/1359

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