Abstract:To investigate the effects of cadmium (Cd) exposure on gut microbiota structures under different dietary habits. The C57BL/6 mice were treated with Normal Diet (ND), Normal diet+10 mg/L CdCl2(ND+Cd), High-Fat Diet (HFD), or High-Fat Diet+10 mg/L CdCl2(HFD+Cd), respectively for 20 weeks. Subsequently, the changes of composition and structure of the gut microbiota were analyzed via 16S rRNA high-throughput sequencing technology. The results indicate that HFD and Cd caused intestinal disorders. Compared with the ND group, HFD intake decreased Bacteroidetes abundance by 2.28 times, and the Bifidobacterium number increased by 14.34 times. Cd intake increased the abundance of Verrucomicrobia, dominated by Akkermansia to about 4.67% of gut microbiota. Compared with the HFD group, the abundance of Actinobacteria in the HFD+Cd group decreased by 4.57 times, RuminococcaceaeUCG-014, Alistipes, Lachnospiraceae_NK4A136_gup, Blautia, and Desulfovibrio being the dominant flora. Studies have shown that HFD or/and Cd can disrupt the balance of gut microbiota, and the intake of Cd under different dietary habits shows a different flora structure, which is reflected in each classification level.
覃一书, 保欣晨, 汪洁, 于淼, 田稳, 张梦研, 向萍. 不同饮食习惯下镉摄入对肠道菌群结构的影响[J]. 中国环境科学, 2021, 41(8): 3896-3905.
QIN Yi-shu, BAO Xin-chen, WANG Jie, YU Miao, TIAN Wen, ZHANG Meng-yan, XIANG Ping. Effect of cadmium intake on the structure of gut microbiota under different dietary habits. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(8): 3896-3905.
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