Abstract：The seepage characteristics of porous media varies with the inner biofilm growth irritated by recharge water during groundwater recharge. Indoor soil column experiments were conducted to stimulate the bio clogging process of porous media by continuous injection with nutrient solutions. Growth morphology biofilm and the corresponding seepage parameters of media (including permeability coefficient, water retention and dispersion coefficient) were measured over a series of time periods during experiments. The results showed that the permeability coefficient slightly decreased and rose in the first 5hours. In 5~18 hours, the permeability coefficient decreased sharply, and the hydraulic dispersion coefficient, and greatly increased, but the change of water holding capacity was not obvious. After 18hours, the decrease rate of the permeability coefficient slowed down, whist the water holding capacity began to increase rapidly, and the solute transport in the porous media transited be recharged by dominant mechanism of dispersion. To sum up, the clogging process by the growth of biofilm in the medium can be divided into three stages:(1) First stage is the adaptation period of bacteria, in which the inoculated bacteria did not dramatically grow and reproduce, and the permeability coefficient changed simply because of the retention and migration of bacteria cells; (2) Second stage is the period of mass growth and reproduction of bacteria, in which the increasing bacteria cells occupy more and more pore volume of medium, resulting the decrease in permeability and the increase in dispersion of the porous media; (3) Last stage is the period of extracellular polymer secretion, in which a permeable biofilm is gradually formed, and the growth and shedding of biofilm forms a dynamic balance with the nutrients provided by recharge water.
王茜丹, 路莹, 杨悦锁, 武宇辉, 徐喆, 裴子丞. 微生物堵塞过程中生物膜生长特征对多孔介质渗流特征影响[J]. 中国环境科学, 2022, 42(6): 2771-2778.
WANG Qian-dan, LU Ying, YANG Yue-suo, WU Yu-Hui, XU Zhe, PEI Zi-cheng. A study of microbial clogging on the variation of seepage characteristics with biofilm growth in porous medium. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(6): 2771-2778.
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