代森锰及其代谢物的神经毒性影响与机制研究

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摘要以SH-SY5Y和PC12细胞为实验模型,深入探讨了代森锰(Maneb)及其代谢产物对多巴胺能神经细胞的毒性影响与机制.结果表明,Maneb的有机部分和金属离子成分单独暴露不具有明显的毒性作用,联合暴露具有协同效应,且对细胞活性的抑制程度与同一浓度水平的Maneb相当,其原因与诱导活性氧自由基生成、细胞凋亡相关.此外,蛋白免疫印迹法发现,Maneb下调Bcl-2的表达、上调Bax、细胞色素C的水平,同时激活Caspase-3,提示线粒体凋亡途径在Maneb诱导多巴胺能神经细胞凋亡过程中的重要作用.

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	刘朝阳
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	杨伯元

关键词 ：代森锰, SH-SY5Y细胞, PC12细胞, 氧化应激, 细胞凋亡

Abstract：The toxicological effects and mechanisms of Maneb and its metabolites on dopaminergic cells were investigated in depth using SH-SY5Y cells and PC12 cells as experimental samples. The results showed that the organic and metal ion components of Maneb did not have obvious toxic effects when exposed alone, but had synergistic effects when exposed in combination, and the degree of inhibition to cells viability was comparable to that of Maneb at the same concentration level. The reasons for this were related to the generation of reactive oxygen species (ROS) and apoptosis. In addition, the Western Blotting revealed that Maneb reduced the expression of Bcl-2, elevated the level of Bax and Cytochrome C and activated Caspase-3 in a dose-dependent manner, suggesting an important role of the mitochondrial apoptosis pathway in dopaminergic cells apoptosis induced by Maneb.

Key words： Maneb SH-SY5Y cells PC12 cells oxidative stress cell apoptosis

收稿日期: 2022-02-18

PACS:

X171.5

基金资助:国家自然科学基金优秀青年基金项目(81822016),国家自然科学基金面上项目(81571249),中国博士后科学基金项目(2020M672420),高等学校学科创新引智基地(B21038)

通讯作者: 刘朝阳,副教授,lcy@zuel.edu.cn E-mail: lcy@zuel.edu.cn

作者简介: 刘朝阳(1986-),男,湖北洪湖人,副教授,博士,主要研究方向为环境毒理与健康、环境健康风险评估.发表论文近40篇.

引用本文:

刘朝阳, 刘泽华, 方艳艳, 杨伯元. 代森锰及其代谢物的神经毒性影响与机制研究[J]. 中国环境科学, 2022, 42(9): 4399-4408. LIU Chao-yang, LIU Ze-hua, FANG Yan-yan, YANG Bo-yuan. Neurotoxic effects and mechanisms of Maneb and its metabolites. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(9): 4399-4408.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2022/V42/I9/4399

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