Effects of the combination of two typical antibiotics on denitrification process in groundwater
LI Wei, HE Jiang-tao, DENG Lu
Key Laboratory of Water Resources and Environmental Engineering, School of Water Resources and Environment, China University of Geosciences, Beijing 100083, China
Abstract：Ciprofloxacin (CIP) and lomefloxacin (LOM) were selected as typical fluoroquinolone antibiotics, sodium acetate was used as the carbon source, and batch experiments were carried out to explore the effects of CIP or/and LOM on denitrification process. Then the influencing mechanisms were investigated by measuring the changes of indicators, such as the number and activity of denitrifying bacteria and the denitrifying enzyme activity. The results showed that compared with the blank system, LOM (100μg/L) group, CIP (100μg/L) group and LOM (50μg/L)+CIP(50μg/L) group had inhibitory effects on degradation both NO3--N and NO2--N. The inhibition rates followed an order of LOM + CIP > CIP > LOM. Antibiotic exposure could inhibit the quantity and activity of microorganisms and the activity of key denitrifying enzymes to varying degrees. The NO3--N degradation was affected by the activity of denitrifying bacteria and the activity of nitrate reductase (NaR), while the NO2--N degradation was mainly affected by the activity of nitrite reductase (NiR). The results of the combined effects of antibiotics showed that the inhibition rates of LOM + CIP group in NO3--N and NO2--N degradation stages were greater than those of single-antibiotic groups, indicating the synergistic inhibition of LOM and CIP; the synergistic inhibition of two typical antibiotics on the activities of key denitrifying enzymes was also observed; however, the inhibition of the combined antibiotics group on the quantity and activity of microorganisms indicated the antagonistic effects. The effects of different antibiotics on denitrification effects were different. LOM mainly affected on the NO3--N degradation stage, while the CIP or LOM+CIP showed similar inhibition effects on both stages of NO3--N degradation and NO2--N degradation.
李薇, 何江涛, 邓璐. 地下水中两种典型抗生素复合对反硝化作用的影响[J]. 中国环境科学, 2022, 42(12): 5703-5712.
LI Wei, HE Jiang-tao, DENG Lu. Effects of the combination of two typical antibiotics on denitrification process in groundwater. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(12): 5703-5712.
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