Parameter optimization for the achievement of nitrite accumulation by denitrifying phosphorus removal process
DI Fei1,2, SUI Qian-wen1, GAO Chao-long1,2, ZHONG Hui1, XU Dong-yao2, WEI Yuan-song1,3
1. Laboratory of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. China University of Mining &Technology, Beijing School of Chemical and Environmental Engineering, Beijing 100089, China; 3. Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330096, China
Abstract:To promote the coupling of denitrification phosphorus removal and anaerobic ammonia oxidation process, and achieve simultaneous efficient removal of nitrogen and phosphorus from wastewater, a sequencing batch reactor (SBR) was constructed to optimized the operational parameters for the nitrite accumulation by denitrifying phosphorus removal process. Under anaerobic-anoxic-microaerobic operational conditions, SBR gradually realized nitrite accumulation during denitrifying phosphorus removal process by feeding synthetic nitrate industrial wastewater in the anoxic section. The results showed that after 142 days of cultivation and domestication, when the influent C/P ratio of 55 and the nitrate concentration in the anoxic section was 23mg/L, the nitrite accumulation rate achieved 51.01%, the NO3--N→NO2--N transformation rate was 40.22%, nitrate removal rate was 72.14% and the highest removal rate of PO43--P was 88.17%. The effluent COD concentration was lower than 25mg/L, and COD removal rate was maintained above 90%. Analysis of the microbial community structure showed that Bacteroidetes, Proteobacteria, Chloroflexi and Acidobacteria were the dominant phylum in the system. Through parameter optimization, the abundance of denitrifying phosphorus accumulating organisms represented by Candidatus Accumulibacter increased (the cumulative abundance increased from 1.49% to 5.08%), and a more remarkable increase in the abundance of glycogen accumulating organisms represented by Candidatus Competibacter (from 1.02% to 15.49%). The combined effects of phosphorus accumulating organisms and glycogen accumulating organisms contributed to the accumulation of nitrite in the phosphorus removal process.
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