Parameter optimization for the achievement of nitrite accumulation by denitrifying phosphorus removal process
DI Fei1,2, SUI Qian-wen1, GAO Chao-long1,2, ZHONG Hui1, XU Dong-yao2, WEI Yuan-song1,3
1. Laboratory of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. China University of Mining &Technology, Beijing School of Chemical and Environmental Engineering, Beijing 100089, China; 3. Institute of Energy, Jiangxi Academy of Sciences, Nanchang 330096, China
Abstract：To promote the coupling of denitrification phosphorus removal and anaerobic ammonia oxidation process, and achieve simultaneous efficient removal of nitrogen and phosphorus from wastewater, a sequencing batch reactor (SBR) was constructed to optimized the operational parameters for the nitrite accumulation by denitrifying phosphorus removal process. Under anaerobic-anoxic-microaerobic operational conditions, SBR gradually realized nitrite accumulation during denitrifying phosphorus removal process by feeding synthetic nitrate industrial wastewater in the anoxic section. The results showed that after 142 days of cultivation and domestication, when the influent C/P ratio of 55 and the nitrate concentration in the anoxic section was 23mg/L, the nitrite accumulation rate achieved 51.01%, the NO3--N→NO2--N transformation rate was 40.22%, nitrate removal rate was 72.14% and the highest removal rate of PO43--P was 88.17%. The effluent COD concentration was lower than 25mg/L, and COD removal rate was maintained above 90%. Analysis of the microbial community structure showed that Bacteroidetes, Proteobacteria, Chloroflexi and Acidobacteria were the dominant phylum in the system. Through parameter optimization, the abundance of denitrifying phosphorus accumulating organisms represented by Candidatus Accumulibacter increased (the cumulative abundance increased from 1.49% to 5.08%), and a more remarkable increase in the abundance of glycogen accumulating organisms represented by Candidatus Competibacter (from 1.02% to 15.49%). The combined effects of phosphorus accumulating organisms and glycogen accumulating organisms contributed to the accumulation of nitrite in the phosphorus removal process.
杜睿,彭永臻.城市污水生物脱氮技术变革:厌氧氨氧化的研究与实践新进展[J].中国科学:技术科学, 2021,52(3):389-402.Du R, Peng Y Z. Technical revolution of biological nitrogen removal from municipal wastewater recent advances in Anammox research and application.[J]. Scientia Sinica Technological, 2021,52(3):389-402.
[2]
韦佳敏,刘文如,程洁红,等.反硝化除磷的影响因素及聚磷菌与聚糖菌耦合新工艺的研究进展[J].化工进展, 2020,39:4608-4618.Wei J M, Liu W R, Cheng J H et al. Influencing factors of denitrifying phosphorus removal and advance research on novel process of coupling PAOs and GAOs[J]. Chemical Industry and Engineering Progress, 2020,39:4608-4618.
[3]
Liu Y, Peng L, Chen X, et al. Mathematical modeling of nitrous oxide production during denitrifying phosphorus removal process[J]. Environmental Science& Technology, 2015,49:8595-8601.
[4]
王秋颖,于德爽,赵骥,等.PD-DPR系统处理城市污水与高硝酸盐废水实现稳定亚硝酸盐积累和磷去除的特性[J].环境科学, 2020, 41(3):1384-1392.Wang Q Y, Yu D S, Zhao J, et al. Stable nitrite accumulation and phosphorus removal from high-nitrate and municipal wastewaters in a combined process of partial denitrification and denitrifying phosphorus removal (PD-DPR)[J]. Environmental Science, 2020, 41(3):1384-1392.
[5]
Kerrn-Jespersen J P. Biological phosphorus uptake under anoxic and aerobic conditions. Water Research[J]. 1993,27(4):617-624.
[6]
Kuba T, Smolders M C M V L G, Heijnen J J. Biological phosphorus pemoval from wastewater by anaerobic-anoxic sequencing batch reactor. Water Science Technology[J]. 1993,27(5/6):241-252.
[7]
卢瑞朋,徐文江,李安峰,等.新型多级缺氧-好氧工艺的反硝化除磷效能及微生物群落特征[J].中国环境科学, 2022,42(4):1706-1713.Lu R P, Xu W J, Li A F,et al. Denitrify phosphorus removal performance and microbial community characteristics of novel multistage anoxic-oxicprocess[j]. China Environmental Science, 2022,42(4):1706-1713.
[8]
Sengar A, Aziz A, Farooqi I H, et al. Development of denitrifying phosphate accumulating and anammox micro-organisms in anaerobic hybrid reactor for removal of nutrients from low strength domestic sewage[J]. Bioresource Technology, 2018,267:149-157.
[9]
Wang X, Wang S, Zhao J, et al. A novel stoichiometries methodology to quantify functional microorganisms in simultaneous (partial) nitrification-endogenous denitrification and phosphorus removal (SNEDPR). Water Research[J]. 2016,95:319-329.
[10]
Sui Q, Jiang L, Di F, et al. Multiple strategies for maintaining stable partial nitritation of low-strength ammonia wastewater. Science of the Total Environmental[J]. 2020,742:140542.
[11]
Sui Q, Di F, Zhang J, et al. Advanced nitrogen removal in a fixed-bed anaerobic ammonia oxidation reactor following an anoxic/oxic reactor:nitrogen removal contributions and mechanisms[J]. Bioresource Technology, 2021,320:124297.
[12]
Smolders G J, Meij J, Loosdrecht M C M V, et al. Model of the anaerobic metabolism of the biological phosphorus removal process:stoichiometry and pH influence[J]. Biotechnology Bioengineing, 1994,43:461-470.
[13]
甄建园,于德爽,王晓霞,等.进水C/P对SNEDPR系统脱氮除磷性能的影响[J].环境科学, 2019,40(1):343-351.Zhen J Y, Yu D S, Wang X X, et al. Effect of the influent C/P ratio on the nutrient removal characteristics of the SNEDPR system[J]. Environmental Science, 2019,40(1):343-351.
[14]
王聪,王淑莹,张淼,等.多因素对反硝化除磷过程中COD、N和P的去除分析[J].化工学报, 2015,66:1467-1475.Wang C, Wang S Y, Zhang M, et al. Analysis of COD, N and P in denitrifying phosphorus removal under multivariate condition[J]. CIESC Journal, 2015,66:1467-1475.
[15]
Du S, Yu D, Zhao J, et al. Achieving deep-level nutrient removal via combined denitrifying phosphorus removal and simultaneous partial nitrification-endogenous denitrification process in a single-sludge sequencing batch reactor[J]. Bioresource Technology, 2019,289:121690.
[16]
Zhang M, Wang Y, Fan Y, et al. Bioaugmentation of low C/N ratio wastewater:effect of acetate and propionate on nutrient removal, substrate transformation, and microbial community behavior[J]. Bioresource Technology, 2019,306:122465.
[17]
Wang X, Zhao J, Yu D, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal, 2019,355:560-571.
[18]
M. Carvalheira, A. Oehmen, G. Carvalho, et al. The impact of aeration on the competition between polyphosphate accumulating organisms and glycogen accumulating organisms[J]. Water Research, 2014,66:296-307.
[19]
He Q, Song Q, Zhang S, et al. Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sequencing batch reactor with mixed carbon sources:reactor performance, extracellular polymeric substances and microbial successions[J]. Chemical Engineering Journal, 2018,331:841-849.
[20]
Zeng W, Bai X, Guo Y, et al. Interaction of"Candidatus Accumulibacter" and nitrifying bacteria to achieve energy-efficient denitrifying phosphorus removal via nitrite pathway from sewage[J]. Enzyme and Microbial Technology, 2017,105:1-8.
[21]
范亚骏,张淼,季俊杰,等.A2/O-BCO系统中碳源类型对反硝化除磷及菌群结构的影响[J].中国环境科学, 2022,42(1):172-182.Fan Y J, Zhang M, Ji J J, et al. Effect of carbon source types on denitrifying phosphorus removal and microbial community in the A2/O-BCO process[J]. China Environmental Science, 2022,42(1):172-182.
[22]
Xu X, Qiu L, Wang C, et al. Achieving mainstream nitrogen and phosphorus removal through simultaneous partial nitrification, anammox, denitrification, and denitrifying phosphorus removal (SNADPR) process in a single-tank integrative reactor[J]. Bioresource Technology, 2019,284:80-89.
[23]
Dai Y, Yuan Z, Wang X, et al. Anaerobic metabolism of Defluviicoccusvanus related glycogen accumulating organisms (GAOs) with acetate and propionate as carbon sources[J]. Water Research, 2007,41(9):1885-1896.
[24]
Ji J, Peng Y, Li X, et al. A novel partial nitrification-synchronous anammox and endogenous partial denitrification (PN-SAEPD) process for advanced nitrogen removal from municipal wastewater at ambient temperatures[J]. Water Research, 2020,175:115690.
[25]
Wang X, Zhao J, Yu D, et al. Evaluating the potential for sustaining mainstream anammox by endogenous partial denitrification and phosphorus removal for energy-efficient wastewater treatment[J]. Bioresource Technology, 2019,284:302-314.
[26]
J.S. CECH, P. HARTMAN. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal system[J]. Water Research, 1993,27(7):1219-1225.
[27]
F.J. Rubio-Rincon, C.M. Lopez-Vazquez, L. Welles, et al. Cooperation between Candidatus Competibacter and Candidatus Accumulibacter clade I, in denitrification and phosphate removal processes[J]. Water Research, 2017,120:156-164.
[28]
Wang X, Wang S, Xue T, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77:191-200.
[29]
Wang D, Zheng Q, Huang K, et al. Metagenomic and metatranscriptomic insights into the complex nitrogen metabolic pathways in a single-stage bioreactor coupling partial denitrification with anammox[J]. Chemical Engineering Journal, 2020,398.
[30]
Li J, Peng Y, Zhang L, et al. Enhanced nitrogen removal assisted by mainstream partial-anammox from real sewage in a continuous flow A2/O reactor[J]. Chemical Engineering Journal, 2020,400:125893.
[31]
Zhang J, Sui Q, Lu T, et al. Sludge bio-drying followed by land application could control the spread of antibiotic resistance genes[J]. Environment International, 2019,130:104906.
