Abstract：A pot experiment was conducted to investigate the effects of addition wheat straw biochar (BC) in simulated cadmium (Cd) contaminated soil on enzymes activities within soil aggregate fractions. The activities of carbon cycling enzyme and oxidoreductase were detected in rice rhizosphere soil aggregate fractions under the condition of BC and Cd treatments. The results showed that the enzymes activities of aggregate fractions in Cd contaminated soil significantly responded to the 2.5% BC treatment, value index of oxidoreductase activities ranged from 0.522 to 0.792. Under the condition of 2.5% mg/kg exogenous Cd, the activities of protease and catalase of soil aggregate were significantly increased by 121% and 653% respectively, which was compared with the treatment without BC. The activities of carbon cycling enzyme, oxidoreductase and comprehensive enzymes could result in soil aggregating effects in the rice rhizosphere soil with the medium sizes of 0.5 to 1mm. The change curve of enzymatic activities went up firstly, then went down, and became stable finally with the increase of the particle sizes of soil aggregate fractions. Studies have shown that the addition of 2.5% BC had a significant effect on the enzyme activities within rice rhizosphere soil aggregate fractions under the condition of 2.5mg/kg exogenous Cd, the activities of soil enzymes was comprehensively affected by the enzymes type, BC content and particle size of aggregate fractions. After adding BC to the Cd contaminated soil, the change curve of soil enzymes activities presented a "∧" pattern with increasing particle size of soil aggregate fractions.
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