农田土壤地膜源微塑料分离检测方法优化

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (1121 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要制备了含地膜源微塑料的土壤样品,在密度分离法基础上提出以超声处理代替消解处理的样品处理方法并使用配套的定量和鉴别方法分析了样品的微塑料指标.结果表明,40kHz、30min的超声处理能够有效去除地膜源微塑料表面杂质并满足定量鉴别要求,也没有对微塑料产生二次破碎风险,最终微塑料回收率为92.9%;相比于消解处理,超声处理可以更大程度保留地膜源微塑料的原始表面形态以及分子结构等特征,并且不需进行繁复的移液、静置操作,使单次分离的总试剂成本降低80%以上,分离用时缩短12h以上.本研究优化的土壤地膜源微塑料分离检测方法在准确性、经济性、简便性方面具有显著优势,为快速、准确评估土壤地膜源微塑料丰度提供了新的途径.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	白润昊
	崔吉晓
	范瑞琪
	李真
	杨扬
	刘宏金
	何文清

关键词 ：地膜, 微塑料, 土壤, 分离检测, 超声处理

Abstract：An ultrasonic treatment was proposed to replace digestion to separate microplastics from mulch filmed soil samples in this study, and then, the microplastic indexes of the samples were quantified. The results showed that the surface impurities of microplastics derived from mulch film could be effectively removed to meet the quantitative requirements by using ultrasonic treatment at 40kHz and 30min. The original surface morphology and chemical properties of the microplastics could be retained to a greater extent using ultrasonic treatment which avoided complicated pipetting and standing operations. This method had no secondary breakage risk for microplastics and led to a final recovery rate of 92.9%. By optimizing the separation and detection method, the total reagent cost of single separation was reduced by more than 80%, and the separation time was shortened by more than 12hours. Obviously, the optimized separation approach proposed in this study would provide a solution to rapidly and accurately evaluating the abundance of microplastics from mulch filmed soils.

Key words： mulch film microplastics soil separation and detection ultrasonic processing

收稿日期: 2022-10-11

PACS:	X53
	S19

基金资助:国家重点研发计划项目(2021YFD1700700);内蒙古自治区科技计划(2022YFHH0042);中央级公益性科研院所基本科研业务费专项(BSRF202207)

通讯作者: 何文清,研究员,hewenqing@caas.cn E-mail: hewenqing@caas.cn

作者简介: 白润昊(1998-),男,山东泰安人,中国农业科学院农业环境与可持续发展研究所硕士研究生,主要从事土壤地膜源微塑料的环境行为及生态风险方面的研究.82101212287@caas.cn.

引用本文:

白润昊, 崔吉晓, 范瑞琪, 李真, 杨扬, 刘宏金, 何文清. 农田土壤地膜源微塑料分离检测方法优化[J]. 中国环境科学, 2023, 43(5): 2404-2412. BAI Run-hao, CUI Ji-xiao, FAN Rui-qi, LI Zhen, YANG Yang, LIU Hong-jin, HE Wen-qing. Optimization of the separation and detection method for microplastics from mulch film in agricultural soils. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(5): 2404-2412.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I5/2404

[1]	贾彦鹏.我国农业循环经济的发展现状与未来举措[J].宏观经济管理, 2022,(8):50-56,81. Jia Y P. The current situation of and future efforts for Chinese rural circular economy[J]. Macroeconomic Management, 2022,(8):50-56,81.
[2]	Yang Y, Li Z, Yan C, et al. Kinetics of microplastic generation from different types of mulch films in agricultural soil[J]. Science of the Total Environment, 2022,814:152572.
[3]	Huang Y, Liu Q, Jia W, et al. Agricultural plastic mulching as a source of microplastics in the terrestrial environment[J]. Environmental Pollution, 2020,260:114096.
[4]	Huang Y, Zhao Y, Wang J, et al. LDPE microplastic films alter microbial community composition and enzymatic activities in soil[J]. Environmental Pollution, 2019,254:112983.
[5]	Wang J, Huang M, Wang Q, et al. LDPE microplastics significantly alter the temporal turnover of soil microbial communities[J]. Science of the Total Environment, 2020,726:138682.
[6]	Wu C, Pan S, Shan Y, et al. Microplastics mulch film affects the environmental behavior of adsorption and degradation of pesticide residues in soil[J]. Environmental Research, 2022,214:114-133.
[7]	Bläsing M, Amelung W. Plastics in soil:Analytical methods and possible sources[J]. Science of the Total Environment, 2018,612:422-435.
[8]	Hurley R R, Lusher A L, Olsen M, et al. Validation of a method for extracting microplastics from complex, organic-rich, environmental matrices[J]. Environmental Science & Technology, 2018,52(13):7409-7417.
[9]	陈雅兰,孙可,韩兰芳,等.土壤中微塑料的分离及检测方法研究进展[J].土壤学报, 2022,59(2):364-380. Chen Y L, Sun K, Han L F, et al. Separation, identification, and quantification methods in soil microplastics analysis:A review[J]. Acta Pedologica Sinica, 2022,59(2):364-380.
[10]	马思睿,李舒行,郭学涛.微塑料的老化特性、机制及其对污染物吸附影响的研究进展[J].中国环境科学, 2020,40(9):3992-4003. Ma S R, Li S X, Guo X T. A review on aging characteristics, mechanism of microplastics and their effects on the adsorption behaviors of pollutants[J]. China Environmental Science, 2020,40(9):3992-4003.
[11]	邹亚丹,徐擎擎,张哿,等.6种消解方法对荧光测定生物体内聚苯乙烯微塑料的影响[J].环境科学, 2019,40(1):496-503. Zou X D, Xu Q Q, Zhang G, et al. Influence of six digestion methods on the determination of polystyrene microplastics in organisms using the fluorescence intensity[J]. Environmental Science, 2019,40(1):496-503.
[12]	Scheurer M, Bigalke M. Microplastics in Swiss floodplain soils[J]. Environmental Science & Technology, 2018,52(6):3591-3598.
[13]	Cole M, Webb H, Lindeque P K, et al. Isolation of microplastics in biota-rich seawater samples and marine organisms[J]. Scientific Reports, 2014,4:4528.
[14]	Nuelle M, Dekiff J H, Remy D, et al. A new analytical approach for monitoring microplastics in marine sediments[J]. Environmental Pollution, 2014,184:161-169.
[15]	苏园园,祁怀源,丰洁,等.微塑料对牟氏角毛藻的毒理效应[J].海洋环境科学, 2022,41(2):193-199. Su Y Y, Qi H Y, Feng J, et al. Toxicological effects of microplastics on Chaetoceros muelleri[J]. Marine Environmental Science, 2022,41(2):193-199.
[16]	王志超,孟青,李卫平,等.不同消解方法对微塑料质量及其表面特征的影响[J].环境工程学报, 2020,14(5):1385-1393. Wang Z C, Meng q, Li W P, et al. Effect of different digestion methods on microplastic quality and surface characteristics[J]. Chinese Journal of Environmental Engineering, 2020,14(5):1385-1393.
[17]	王荐,吴运金,何跃,等.通过屋顶暴露方法模拟聚碳酸酯微塑料在长江水样的长期老化行为[J].环境科学学报, 2022,42(5):336-344. Wang J, Wu Y J, He Y, et al. Long-term aging behavior of polycarbonate microplastic in the Yangtze River via a rooftop exposure protocol[J]. Acta Scientiae Circumstantiae, 2022,42(5):336-344.
[18]	许焕征,解清杰,尹海龙,等.污水厂污水中微塑料的消解方法[J].科学技术与工程, 2020,20(2):860-865. Xu H Z, Xie Q J, Yin H L, et al. Digestion method of microplastics in wastewater from wastewater treatment plant[J]. Science Technology and Engineering, 2020,20(2):860-865.
[19]	周倩,涂晨,张晨捷,等.滨海湿地环境中微塑料表面性质及形貌变化[J].科学通报, 2021,66(13):1580-1591. Zhou Q, Tu C, Zhang C J, et al. Surface properties and changes in morphology of microplastics exposed in-situ to Chinese coastal wetlands[J]. Chinese Science Bulletin, 2021,66(13):1580-1591.
[20]	张晨捷,涂晨,周倩,等.低密度聚乙烯薄膜微塑料在黄河口海岸带环境中的风化特征[J].土壤学报, 2021,58(2):456-463. Zhang C J, Tu C, Zhou Q, et al. Weathering characteristics of microplastics of low-density polyethylene film in the coastal environment of the Yellow River Estuary[J]. Acta Pedologica Sinica, 2021,58(2):456-463.
[21]	李丹文,林莉,潘雄,等.淡水环境中微塑料采样及预处理方法研究进展[J].长江科学院院报, 2021,38(7):14-23. Li D W, Lin L, Pan X, et al. Research progress on sampling and pretreatment methods of microplastics in fresh water environment[J]. Journal of Yangtze River Scientific Research Institute, 2021,38(7):14-23.
[22]	宋小卫,吴晓凤,宋小平,等.微塑料的提取分离方法研究进展[J].环境化学, 2022,41(3):793-800. Song X W, Wu X F, Song X P, et al. Research progress on the extraction and separation methods of microplastics[J]. Environmental Chemistry, 2022,41(3):793-800.
[23]	Wagner J, Wang Z, Ghosal S, et al. Novel method for the extraction and identification of microplastics in ocean trawl and fish gut matrices[J]. Analytical Methods, 2017,9(9):1479-1490.
[24]	吴文楠,高俊敏,沈茜,等.生物体微塑料提取方法比选研究[J].中国环境科学, 2019,39(10):4343-4349. Wu W N, Gao J M, Shen X, et al. Comparison of microplastic extraction methods from organisms[J]. China Environmental Science., 2019,39(10):4343-4349.
[25]	纪玲玲.AM真菌驱动的土壤团聚体形成过程与稳定机制[D].武汉:华中农业大学, 2020. Ji L L. The formation and stabilization mechanisms of soil aggregates driven by arbuscular mycorrhizal fungi[D]. Wuhan:Huazhong Agricultural University, 2020.
[26]	汪敏,徐磊,严旎娜,等.设施内PBAT/秸秆基地膜降解特性及对番茄生长的影响[J].农业工程学报, 2022,38(5):184-193. Wang M, Xu L, Yan N N, et al. PBAT/straw based mulching film degradation characteristics and effect on tomato growth in facilities[J]. Transactions of the Chinese Society of Agricultural Engineering, 2022, 38(5):184-193.
[27]	van Schothorst B, Beriot N, Lwanga E H, et al. Sources of light density microplastic related to two agricultural practices:The use of compost and plastic mulch[J]. Environments, 2021,8(4):36.
[28]	Prosenc F, Leban P, Sunta U, et al. Extraction and identification of a wide range of microplastic polymers in soil and compost[J]. Polymers, 2021,13(23):4069.
[29]	Liu M, Song Y, Lu S, et al. A method for extracting soil microplastics through circulation of sodium bromide solutions[J]. Science of the Total Environment, 2019,691:341-347.
[30]	Grause G, Kuniyasu Y, Chien M, et al. Separation of microplastic from soil by centrifugation and its application to agricultural soil[J]. Chemosphere, 2022,288:132654.
[31]	Yang J, Li R, Zhou Q, et al. Abundance and morphology of microplastics in an agricultural soil following long-term repeated application of pig manure[J]. Environmental Pollution, 2021,272:116028.
[32]	李真,何文清,杨扬,等.从土壤中快速分离检测地膜源微塑料的方法:中国, CN114062071A[P]. 2022-02-18. Li Z, He W Q, Yang Y, et al. Methods for rapid separation and detection of microplastics derived from plastic film from soil:China, CN114062071A[P]. 2022-02-18.
[33]	Liu M T, Song Y, Lu S B, et al. A method for extracting soil microplastics through circulation of sodium bromide solutions[J]. Science of the Total Environment, 2019,691:341-347.
[34]	Cohen Q M, Glaese M, Meng K, et al. Parks and recreational areas as sinks of plastic debris in urban sites:The case of light-density microplastics in the city of Amsterdam, the Netherlands[J]. Environments, 2022,9(1):5.
[35]	Corradini F, Casado F, Leiva V, et al. Microplastics occurrence and frequency in soils under different land uses on a regional scale[J]. Science of the Total Environment, 2021,752:141917.
[36]	Zobkov M B, Esiukova E E. Evaluation of the Munich Plastic Sediment Separator efficiency in extraction of microplastics from natural marine bottom sediments[J]. Limnology and Oceanography:Methods, 2017,15(11):967-978.
[37]	章海波,周倩,骆永明,等.一种微颗粒塑料的连续流动分离浮选装置及方法:中国, CN106179773A[P]. 2018-09-21. Zhang H B, Zhou Q, Luo Y M, et al. The utility model relates to a device and a method for continuous flow separation and flotation of microparticle plastics:China, CN106179773A[P]. 2018-09-21.
[38]	林婧,李振国,余光辉,等.密度分离法提取土壤中微塑料的优化[J].中国环境科学, 2022,42(7):3285-3294. Lin J, Li Z G, Yu G H, et al. Optimization of microplastics extraction from soil by density separation[J]. China Environmental Science, 2022,42(7):3285-3294.
[39]	刘梦婷.上海郊区主要类型土壤中微塑料污染特征研究[D].上海:华东师范大学, 2020. Liu M T. Study on characteristics of microplastic pollution inmain types of soil in suburb of Shanghai[D]. Shanghai:East China Normal University, 2020.