氨氮浓度对生物膜磷富集效果的影响

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摘要在厌氧/好氧运行的聚磷生物膜反应器中,考察模拟污水中氨氮浓度对生物膜磷富集效果以及吸、释磷速率的影响.结果表明:当模拟污水中的氨氮浓度由15mg-N/L提高至30mg-N/L,生物膜中聚磷菌(PAOs)的吸磷速率略微下降,但由于反硝化聚磷菌(DPAOs)的吸磷速率加快,生物膜系统整体的吸、释磷速率有所提高,分别达到11.25和12.02mg-P/(L·h);富集液浓度达到52mg-P/L;继续提高氨氮浓度为40mg-N/L时PAOs的磷代谢活性几乎被完全抑制,此时生物膜内的吸、释磷速率主要依靠DPAOs,生物膜系统的吸、释磷速率有所下降,分别为8.65和8.81mg-P/(L·h),富集液浓度仅为40mg-P/L.化学计量学分析显示:当氨氮浓度由15mg-N/L升高至40mg-N/L时,生物膜系统P_rel/HAc_upt(吸收单位量的碳源所释放的磷)呈现先升后降的变化规律,平均值分别为0.06,0.11和0.07P-mmol/C-mmol,这表明聚磷生物膜释磷过程对碳源的利用效率也受到氨氮浓度变化的影响.通过降低厌氧阶段进入反应器的模拟污水与富集液体积比(由3:1~1.5:1),可以有效改善高浓度氨氮(40mg-N/L)对生物膜的不利影响,生物膜系统的吸、释磷速率分别提升至16.25和15.60mg-P/(L·h),富集液浓度稳定在70mg-P/L,同时P_rel/HAc_upt提高至0.29P-mmol/C-mmol,磷富集效果得到显著强化.本研究的结果表明,采用生物膜法对模拟城市污水中的磷进行富集,其富集效果不会受污水中氨氮(浓度通常不超过40mg/L)的影响,且系统总氮去除率可达到95%,该技术具有实现同步富集磷与去除氮的潜力.

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关键词 ：氨氮浓度, 生物膜反应器, 吸/释磷速率, P_rel/HAc_upt

Abstract：In this paper, the effects of ammonia concentration in synthetic sewage on the phosphorus (P) enrichment performance, P uptake and release rate of biofilm were investigated in an anaerobic/aerobic polyphosphate accumulating organisms biofilm reactor. The results showed that an increase of ammonia from 15mg-N/L to 30mg-N/L stimulated the P uptake rate of denitrifying polyphosphate accumulating organisms while weaken the PAOs, which resulted in the phosphate uptake rates (PUR) of 11.25mg-P/(L·h),and phosphate release rate (PRR) of 12.02mg-P/(L·h) for the biofilm system. The P concentration in enriched stream reached 52mg-P/L. The uptake and release of P mainly depended on DPAOs when further increase of ammonia to 40mg-N/L, whereas the PAOs metabolism was almost suppressed. As a consequence, the P concentration in enrichment stream was only 40mg-P/L with 8.65mg-P/(L·h) of PUR and 8.81mg-P/(L·h) of PRR. Stoichiometric analysis showed the P_rel/HAc_upt(the P released per organics uptake) of the biofilm system witnessed a rising and decent trend, with an increase of ammonia from 15mg-N/L to 40mg-N/L. The average values of P_rel/HAc_uptunder different ammonia concentrations were 0.06, 0.11and 0.07P-mmol/C-mmol, respectively. The adverse effect of ammonia (40mg-N/L) can be eliminat by decrease the volume ratio of sewage stream and enrichment stream (from 3:1 to 1.5:1). Specifically, the PUR and PRR of biofilm reached 16.25mg-P/(L·h) and 15.60mg-P/(L·h) respectively, resulting in a enrichment stream of 70mg-P/L. Meanwhile, the P_rel/HAc_upt rised up to 0.29P-mmol/C-mmol. The resuls of present study showed that the P enrichment performance in PAOs-biofilm was not affected by ammonia (< 40mg-N/L), also, the nitrogen removal efficiency reached 95%. Therefore, PAOs-biofilm-based technology is of great potential in simultaneous P enrichment and nitrogen removal from sewage.

Key words： ammonia concentration biofilm sequencing batch reactor phosphorus uptake and release rates P_rel/HAc_upt

收稿日期: 2022-12-06

PACS:

X703.1

基金资助:国家自然科学基金资助项目(51938010)

通讯作者: 毕贞,副教授,bizhen@mail.usts.edu.cn E-mail: bizhen@mail.usts.edu.cn

作者简介: 毕贞(1986-),女,江苏苏州人,副教授,博士,研究方向为废水处理及其资源化.发表论文20余篇.bizhen@mail.usts.edu.cn

引用本文:

毕贞, 张胜, 付豪, 丁若琳, 黄勇. 氨氮浓度对生物膜磷富集效果的影响[J]. 中国环境科学, 2023, 43(7): 3447-3453. BI Zhen, ZHANG Sheng, FU Hao, DING Ruo Ling, HUANG Yong. Effect of ammonia nitrogen concentration on phosphorus enrichment from biofilm. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3447-3453.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I7/3447

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