Effect of biochar on the removal of nitrate and N2O emission from water by a screened denitrifying bacterium
WANG Chao-xu1,2, LI Yuan-kun1, LIU Yong-chao1
1. College of Environmental Science and Engineering, Taiyuan University of Technology, Jinzhong 030600, China; 2. Innovation Center for Postgraduate Education in Municipal Engineering of Shanxi Province, Jinzhong 030600, China
Abstract：To investigate the effect of modified biochar on microbial removal of nitrate with low concentration and N2O emission from water, FeCl3-, H2O2- and NaOH-modified biochars (BC-Fe, BC-H2O2, and BC-NaOH) were prepared by utilizing the pristine rice husk-derived biochar (BC) and characterized further. Meanwhile, a strain of denitrifying bacteria, Raoultella ornithinolytica strain KK1, was screened from activated sludge. Then BC, BC-Fe, BC-H2O2, and BC-NaOH were incorporated into the incubation system containing the strain, respectively and a control treatment without any biochar addition was also setup. The indoor incubation experiment on the microbial removal of nitrate (about 10mg/L, in terms of N) from simulated wastewater was carried out and the dynamic changes of N2O emission rate and NO3--N, NO2--N, NH4+-N, TN, and dissolved organic carbon (DOC) concentrations during incubation were analyzed. Results showed that the average NO3--N degradation rate of the screened strain was 0.10mg/(L·h) within 120h. Compared with BC, the carboxyl and lactone contents of BC-Fe and BC-H2O2 significantly increased and their oxidizing ability was enhanced, while the phenolic hydroxyl content of BC-NaOH significantly increased and its reducing ability was enhanced. Meanwhile, the total acidic oxygen-containing functional group contents of BC-Fe and BC-H2O2 significantly increased by 57.17% and 22.86%, respectively compared with BC, while the total basic oxygen-containing functional group content of BC-NaOH significantly increased by 91.67%. Compared with BC, BC-Fe and BC-H2O2 promoted the reduction of NO3--N by denitrifier, and the cumulative N2O emissions were 3.11 and 2.17 times higher than that of the treatment with BC addition, respectively; while BC-NaOH inhibited the reduction of NO3--N by denitrifier, and the cumulative N2O emission was only 49.63% that of the treatment with BC addition. The impact of biochar on microbial denitrification and N2O emission is attributed to the differences in pH value, DOC content, and redox property of biochar caused by modification.
王朝旭, 李元坤, 刘勇超. 生物炭对筛选菌株去除水中硝酸盐和N2O排放的影响[J]. 中国环境科学, 2023, 43(11): 5728-5736.
WANG Chao-xu, LI Yuan-kun, LIU Yong-chao. Effect of biochar on the removal of nitrate and N2O emission from water by a screened denitrifying bacterium. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(11): 5728-5736.
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