Nitrite accumulation characteristics in a SO32--driven partial autotrophic denitrification process
NIE Yu-ting1,2, ZHOU Xin1,2, PING Cai-xia1,2
1. College of Environmental Science and Engineering, Taiyuan University of Technology, Jinzhong 030600, China; 2. Innovation Center for Postgraduate Education in Municipal Engineering of Shanxi, Jingzhong 030600, China
Abstract：This study developed a novel process of sulfite-driven autotrophic partial denitrification (SDAPD) to achieve the rapid accumulation of NO2--N. Anaerobic sequencing batch biofilm reactors were used to explore the effects on nitrite accumulation under different influent nitrate concentration (25~250mg/L) and different S/N molar ratios (0.8,1.7,2.6). The results show that nitrite accumulation rate (NAR) increased with the increase of influent nitrate concentration, and the S/N can significantly affect the NAR. The NAR was the highest up to (64.7%±3.0%) at S/N of 1.7. Periodic experiments show that nitrate reduction and nitrite accumulation were accompanied by sulfite removal and sulfate production. High S/N could promote the production of protein and polysaccharide in extracellular polymer (EPS) and increase the ratio of PN/PS. Three-dimensional fluorescence spectra shows that the tryptophan was the main component of EPS in SDAPD system, and its fluorescence peak and fluorescence intensity were closely related to S/N. High-throughput sequencing found that Thiobacillus and Thermomonas were key bacteria with the function of sulfur autotrophic denitrification.
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