Characteristics and influencing factors of N2O emission from incubated soil under aerated irrigation
LEI Hong-jun1, LIU Huan1, ZANG Ming1, PAN Hong-wei1, CHEN De-li2
1. School of Water Conservancy, North China University of Water Conservancy and Electric Power, Zhengzhou 450046, China;
2. Faculty of Land and Food Resources, University of Melbourne, Victoria 3010, Australia
To clarify the characteristics of soil N2O emission and identify the main factors under aerated irrigation (AI), the soil culture experiments were conducted at 2 irrigation levels with upper soil moisture limit as 70% and 90% of field capacity and 2 dissolved oxygen (DO) levels at 5 and 40mg/L. Soil N2O emission fluxes were monitored using static chamber-gas chromatography method and the copy number of nitrification and denitrification gene were determined using the real-time quantitative polymerase chain reaction (qPCR) technique. In addition, the main factors on soil N2O fluxes were analyzed, including soil water filling porosity (WFPS), DO, redox potential (Eh), mineral nitrogen content (NO3--N and NH4+-N), as well as the abundance of soil ammonia-oxidizing bacterial (AOB) and ammonia-oxidizing archaea (AOA) and denitrifier genes (narG and nosZ). Results showed that soil N2O flux increased from the beginning, peaked at 1d after irrigation, dropped in the following 3days, and then stabilized. An increase of aeration and irrigation amount resulted in the increase of average values and peak values of soil N2O fluxes. Irrigation caused an increase of WFPS, while a decrease of soil DO and Eh. Aeration treatments increased soil DO concentration and Eh (P<0.05), improved soil aeration. However, aeration treatments showed no significant impact on WFPS. The WFPS, Eh and NO3--N content were the main physical, chemical influencing factors driving soil N2O emission under AI. The AI significantly affected AOA copy numbers. In addition, soil N2O fluxes were significantly correlated with AOA copy number (P<0.05). The results could provide scientific support for the influential mechanism of AI on soil N2O and the farmland N2O emission management.
雷宏军, 刘欢, 臧明, 潘红卫, 陈德立. 曝气灌溉条件下土壤N2O排放特征及影响因子分析[J]. 中国环境科学, 2019, 39(5): 2115-2122.
LEI Hong-jun, LIU Huan, ZANG Ming, PAN Hong-wei, CHEN De-li. Characteristics and influencing factors of N2O emission from incubated soil under aerated irrigation. CHINA ENVIRONMENTAL SCIENCECE, 2019, 39(5): 2115-2122.
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