工频电磁环境对果蝇发育和节律行为的影响研究

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摘要比较了PF-EMF暴露下W1118野生型和Rye突变型果蝇发育、睡眠和运动节律行为的差异.连续暴露处理3d后去除亲本果蝇，记录子代发育过程并监测运动和睡眠节律行为.结果表明，PF-EMF暴露后W1118型成蛹量、成蝇量分别显著提高56.2%和57.5%，Rye型果蝇成蛹量无显著差异，成蝇量显著增加85.2%，子代雌雄比例未显著变化.从行为数据看，两株系果蝇暴露后运动和睡眠节律相位未见显著差异，W1118型表现为24h平均运动次数增加37.3%，24h睡眠片段次数减少10.6%，Rye型24h平均运动次数显著增加50.0%，24h睡眠片段次数减少51.5%，总睡眠时长显著降低45.7%.和W1118型相比，Rye型果蝇发育、运动、睡眠情况改变更显著，提示Rye型果蝇电磁暴露更为敏感.总之PF-EMF环境对昆虫发育和节律行为均可带来影响，造成后代数量上升、显著活跃果蝇运动行为并引起睡眠障碍.本研究为进一步了解电磁暴露对昆虫类生物的影响，合理评估PF-EMF的生态效应提供依据.

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	邹鑫平
	王晓丽
	端正花
	张紫燕
	孙永彦

关键词 ：工频电磁场, 黑腹果蝇, 节律行为, 发育

Abstract：Differences in development, sleep and movement rhythmic behaviors between W1118 wild-type flies and Rye mutants were investigated under PF-EMF exposure. After continuous exposure for 3 days, the parental flies were removed. Developmental process, movement and sleep rhythmic behaviors of offspring were monitored. The results showed that, the pupal and fly population of W1118 significantly increased respectively by 56.2% and 57.5% under PF-EMF exposure. No significant changes were observed in pupal numbers of Rye type flies, but the fly population significantly increased by 85.2%. No significant change of sex ratio was found under PF-EMF exposure. For W1118 flies, the average number of 24-hour movements was increased by 37.3% after PF-EMF exposure, and 10.6% decrease in the number of 24-hour sleep segments was calculated. For Rye type flies, the average counts of 24-hour movement significantly increased by 50.0% after exposure, and 51.5% decrease of 24-hour sleep segment counts was investigated. In the meanwhile, 45.7% decrease of the total sleep duration were also found. Compared with the W1118 type, the Rye mutants showed more significant changes, which suggested that Rye mutants were more sensitive to PF-EMF exposure. In brief, the PF-EMF environment can impact development and rhythmic behaviors of insects. Increased fly offspring numbers movement counts, and disturbed sleep were found after PF-EMF exposure. Further understandings on the entomological effects of PF-EMF exposure, and reasonable evaluations on the ecological effects of PF-EMF can be provided in this study.

Key words： PF-EMF Drosophila melanogaster behavioral rhythms development

收稿日期: 2023-03-09

PACS:

X171.5

基金资助:自然资源部海洋生物遗传资源重点实验室开放课题项目（HY202106）；国家自然科学基金资助项目（32201017）

通讯作者: 孙永彦,讲师,yongyansun@email.tjut.edu.cn E-mail: yongyansun@email.tjut.edu.cn

作者简介: 邹鑫平(1997-),男,江西抚州人,天津理工大学硕士,主要从事物理环境与睡眠健康相关研究.发表论文1篇.zouxinping918@163.com.

引用本文:

邹鑫平, 王晓丽, 端正花, 张紫燕, 孙永彦. 工频电磁环境对果蝇发育和节律行为的影响研究[J]. 中国环境科学, 2023, 43(11): 6149-6155. ZOU Xin-ping, WANG Xiao-li, DUAN Zheng-hua, ZHANG Zi-yan, SUN Yong-yan. Effects of power frequency electromagnetic environment on development and rhythmic behavior of Drosophila melanogaster. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(11): 6149-6155.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2023/V43/I11/6149

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