CO<sub>2</sub>对<i>A. ferrooxidans</i>活性和次生铁矿物形成的影响

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摘要通过摇瓶试验模拟富铁富硫酸盐环境,研究了在0.03%(空气中CO₂含量),3%,6%,9%和12%浓度的CO₂条件下对Acidithiobacillus ferrooxidans活性及次生铁矿物形成的影响,并分析了pH值,Fe²⁺氧化率及氧化速率,总Fe沉淀率以及次生铁矿物矿相等相关指标.结果表明,CO₂浓度为3%时,菌氧化Fe²⁺能力最强,72h时Fe²⁺氧化率达到100%,试验结束时总Fe沉淀率最高,为42.8%.随着CO₂浓度增加,各体系A. ferrooxidans活性受到抑制.不同CO₂浓度体系最终获得矿物均为黄铁矾类矿物混合少量施氏矿物.适当提高CO₂浓度,有助于提高A. ferrooxidans活性,并促进水解成矿增加矿物产量.本研究为酸性矿山废水的治理提供理论依据.

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	黄海涛
	耿康慧
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	王崇
	魏彩春

关键词 ： CO₂, 嗜酸性氧化亚铁硫杆菌, 生物氧化, 次生铁矿物

Abstract：The effects of 0.03% (CO₂ content in air), 3%, 6%, 9% and 12% CO₂ concentrations on the activity of Acidithiobacillus ferrooxidans and the formation of secondary iron minerals were studied using shaking flask experiments to simulate the iron-rich and sulfate-rich environment. The pH, Fe²⁺ oxidation rate and oxidation rate, total Fe precipitation rate and secondary iron mineral phase-related indicators were analysed. The results showed that when the concentration of CO₂ was 3%, the ability of bacteria to oxidise Fe²⁺ was the strongest and the oxidation efficiency of Fe²⁺ reached 100% at 72h. At the end of the experiment, the total Fe precipitation rate was the highest, at 42.8%. With the increase in CO₂ concentration, the activity of A. ferrooxidans in each system was inhibited. The final minerals obtained in different CO₂ concentration systems were all jarosite minerals mixed with a small amount of schwertmannite. An appropriate increase in CO₂ concentration is therefore helpful to improve the activity of A. ferrooxidans and promote hydrolysis mineralisation, thus increasing mineral yield. This study provides a theoretical basis for the treatment of acid mine wastewater.

Key words： CO₂ A.ferrooxidans biooxidation secondary iron minerals

收稿日期: 2023-10-20

PACS:

X172

基金资助:广西环境污染控制理论与技术重点实验室科教结合科技创新基地项目,广西环境污染控制理论与技术重点实验室研究基金资助项目(1601Z005);桂林理工大学科研启动基金资助项目(GUTQDJJ 2005020)

通讯作者: 魏彩春,副教授,weicaichun@126.com E-mail: weicaichun@126.com

作者简介: 黄海涛(1978-),男,壮族,广西崇左人,副教授,博士,主要从事环境污染控制方向研究.发表论文20余篇.hthuang@126.com.

引用本文:

黄海涛, 耿康慧, 伍贤辉, 王崇, 魏彩春. CO₂对A. ferrooxidans活性和次生铁矿物形成的影响[J]. 中国环境科学, 2024, 44(5): 2652-2659. HUANG Hai-tao, GENG Kang-hui, WU Xian-hui, WANG Chong, WEI Cai-chun. Effect of CO₂ on the activity and formation of secondary iron minerals by Acidithiobacillus ferrooxidans. CHINA ENVIRONMENTAL SCIENCECE, 2024, 44(5): 2652-2659.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2024/V44/I5/2652

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