干湿交替和冻融循环促进风化煤固定化微生物材料钝化铅的稳定性

摘要
图/表
参考文献
相关文章 (15)

全文: PDF (3025 KB) HTML (1 KB)
输出: BibTeX | EndNote (RIS)

摘要通过室内模拟试验,分析了干湿交替和冻融循环对土壤中DTPA可提取态Pb (II)含量、Pb形态及材料结构的影响,旨在探究这两种环境因素对风化煤固定化微生物材料修复铅污染土壤效果的稳定性及机制.结果显示:干湿交替35个周期后低浓度铅污染土壤(LS-DW)和高浓度铅污染土壤(HS-DW)中DTPA可提取态Pb (II)含量分别降低46.41%和29.42%;冻融循环35个周期后低浓度铅污染土壤(LS-FT)和高浓度铅污染土壤(HS-FT)中DTPA可提取态Pb (II)含量分别降低40.06%和32.77%;LS-DW、LS-FT处理的残渣态含量增加.结构表征发现,风化煤固定化微生物材料经干湿交替、冻融循环后,表面变粗糙,比表面积、含氧官能团和Pb吸附点位增加,与官能团的络合作用增强,促进了钝化铅污染效果的稳定性.

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	庞黛
	李小针
	孙艳稳
	李林
	焦子乐
	李建华

关键词 ：干湿交替, 冻融循环, 稳定性, 铅污染土壤, 风化煤固定化微生物材料

Abstract：The impacts of dry-rewetting and freeze-thaw cycles on DTPA-extractable Pb(II) content and Pb speciation in soils and material structure of weathered coal-based immobilized microbial materials were investigated through a controlled simulated experiment. This study aimed to explore the mechanisms through which these two factors affect the effectiveness of leadcontaminated soil remediation using selected microbes. The results showed that after 35 dry-rewetting cycles, the DTPA-extractable Pb(II) content in low-concentration lead-contaminated soil (LS) and high-concentration lead-contaminated soil (HS) decreased by 46.41% and 29.42%, respectively, compared to the initial levels. After 35 freeze-thaw cycles, the content in LS and HS decreased by 40.06% and 32.77%, respectively. Additionally, the residual fraction of Pb in LS increased under both dry-rewetting and freeze-thaw treatments. Structural analysis revealed that the surface of weathered coal-became rougher, with increases in specific surface area, oxygen-containing functional groups, and Pb adsorption sites increased after dry-wet and freeze-thaw cycles. These changes enhanced complexation with functional groups thereby improving the stability of the passivation effect on lead contamination.

Key words： dry-rewetting cycle freeze-thaw cycle stability lead-contaminated soil weathered coal-based immobilized microbes

收稿日期: 2024-09-03

PACS:

X53

基金资助:山西省自然科学基金项目(202103021224137);山西农大博士科研启动项目(2021BQ48);国家重点研发项目(2020YFC1806504-03)

作者简介: 庞黛(1998-),女,内蒙古乌兰察布人,硕士研究生,主要从事土壤重金属污染修复研究.15149720885@163.com.

引用本文:

庞黛, 李小针, 孙艳稳, 李林, 焦子乐, 李建华. 干湿交替和冻融循环促进风化煤固定化微生物材料钝化铅的稳定性[J]. 中国环境科学, 2025, 45(5): 2681-2692. PANG Dai, LI Xiao-zhen, SUN Yan-wen, LI Lin, JIAO Zi-le, LI Jian-hua. Dry-rewetting and freeze-thaw cycles enhance the stability of lead passivation by microbes immobilized in weathered coal. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(5): 2681-2692.

链接本文:

http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2025/V45/I5/2681

[1] 余涛,蒋天宇,刘旭,等.土壤重金属污染现状及检测分析技术研究进展[J].中国地质,2021,48(2):460-476.Yu T,Jiang T Y,Liu X,et al.Research progress in current status of soil heavy metal pollution and analysis technology[J].Geology in China,2021,48(2):460-476.
[2] Deng Y Y,Huang S,Laird A D,et al.Adsorption behaviour and mechanisms of cadmium and nickel on rice straw biochars in singleand binary-metal systems[J].Chemosphere,2019,218:308-318.
[3] Wei T,Li X,Li H,et al.The potential effectiveness of mixed bacteria-loaded biochar/activated carbon to remediate Cd,Pb co-contaminated soil and improve the performance of pakchoi plants[J].Journal of Hazardous Materials,2022,435:129006.
[4] Chen H M,Tang L Y,Wang Z J,et al.Evaluating the protection of bacteria from extreme Cd (II) stress by P-enriched biochar[J].Environmental Pollution,2020,263(PA):114483.
[5] Tian D,Jiang Z Q,Jiang L,et al.A new insight into lead (II) tolerance of environmental fungi based on a study of aspergillus niger and penicillium oxalicum[J].Environmental Microbiology,2019,21(1):471-479.
[6] Wu P,Wang Z Y,Amit B,et al.Microorganisms-carbonaceous materials immobilized complexes:Synthesis,adaptability and environmental applications[J].Journal of Hazardous Materials,2021,416:125915.
[7] 杜兆林,陈洪安,姚彦坡,等.生物炭固定化微生物修复污染土壤研究进展[J].农业环境科学学报,2022,41(12):2584-2592.Du Z L,Chen H A,Yao Y P,et al.Research progress on bioremediation of contaminated Soil by biochar immobilized microorganisms[J].Journal of Agricultural Environmental Science,2022,41(12):2584-2592.
[8] 杨宗政,许文帅,吴志国,等.微生物固定化及其在环境污染治理中的应用研究进展[J].微生物学通报,2020,47(12):4278-4292.Yang Z Z,Xu W S,Wu Z G,et al.Research progress on microbial immobilization and its application in environmental pollution control[J].Microbiology Bulletin,2020,47(12):4278-4292.
[9] Liu Q J,Huang Y T,Zhou Y M,et al.Impacts of wet-dry alternations on cadmium and zinc immobilisation in soil remediated with iron oxides[J].Journal of Environmental Management,2022,326(Pt A):116660.
[10] Fang S E,Tsang C D,Zhou F,et al.Stabilization of cationic and anionic metal species in contaminated soils using sludge-derived biochar[J].Chemosphere,2016,149:263-271.
[11] 李迎新.冻融循环和干湿交替对退耕还湿土壤磷形态影响研究[D].长春:中国科学院大学(中国科学院东北地理与农业生态研究所),2021.Li,Y X.Research on the impact of freeze-thaw cycles and wet-dry alternations on phosphorus forms in soil of farmland returned to wetland[D].Changchun:University of Chinese Academy of Sciences (Institute of Northeast Geography and Agroecology,Chinese Academy of Sciences),2021.
[12] 韩露,万忠梅,孙赫阳.冻融作用对土壤物理、化学和生物学性质影响的研究进展[J].土壤通报,2018,49(3):736-742.Han L,Wan Z g,Sun H y.Research progress on the impact of freeze-thaw action on physical,chemical and biological properties of soil[J].Soil Bulletin,2018,49(3):736-742.
[13] Wei M L,Du Y J,Reddy K R,et al.Effects of freeze-thaw on characteristics of new KMP binder stabilized Zn-and Pbcontaminated soils[J].Environmental Science and Pollution Research International,2015,22(24):19473-19484.
[14] Mo H J,Wang L,Wang Y,et al.Mobility of exogenous lead in acidic soil treated with wheat straw biochar after aging process of freeze-thaw cycles[J].Environmental Pollutants and Bioavailability,2022,34(1):253-262.
[15] Li L H,Ma J C,Xu M L,et al.The adsorption and desorption of Pb (2+) and Cd (2+) in freeze-thaw treated soils[J].Bulletin of Environmental Contamination and Toxicology,2016,96(1):107-112.
[16] 韦亮,孙立永,田雪,等.干湿交替下生物炭对复合污染土壤中镉砷有效性的影响[J].水土保持学报,2021,35(1):341-347.Wei L,Sun L Y,Tian X,et al.Effect of biochar on cadmium and arsenic availability in contaminated soil under wet-dry alternations[J].Journal of Soil and Water Conservation,2021,35(1):341-347.
[17] Yang Z P,Chang J Z,Li X Y,et al.The Effects of the long-term freeze–thaw cycles on the forms of heavy metals in solidified/stabilized lead–zinc–cadmium composite heavy hetals contaminated soil[J].Applied Sciences,2022,12(6):2934-2934.
[18] Jiao Z L,Gao C H,Li J H,et al.Weathered coal-immobilized microbial materials as a highly efficient adsorbent for the removal of lead[J].Molecules (Basel,Switzerland),2024,29(3):660.
[19] 焦子乐,李建华,陈潇晶,等.风化煤固定化微生物细胞制备及其固定化机制[J].中国环境科学,2023,43(9):4905-4915.Jiao Z L,Li J H,Chen X J,et al.preparation of immobilized microbial cells using weathered coal and its immobilization mechanism[J].China Environmental Science,2023,43(9):4905-4915.
[20] GB/15618-2018土壤环境质量农用地土壤污染风险管控标准(试行)[S].GB/15618-2018 Soil environmental quality-risk control standard for soil pollution of agricultural land (Trial)[S].
[21] Huang X Y,Lyu P,Li L F,et al.Effect of three aging processes on physicochemical and As (V) adsorption properties of Ce/Mn-modified biochar[J].Environmental research,2022,214(P1):113839-113839.
[22] 马贵,任珺,陶玲,等.混维凹凸棒石对矿区土壤重金属的钝化修复[J].环境科学与技术,2024,47(4):152-158.Ma G,Ren J,Tao L,et al.Immobilization and remediation of heavy metals in mine soil by mixed-dimensional attapulgite[J].Environmental Science and Technology,2024,47(4):152-158.
[23] 白利勇,季慧慧,孙文轩,等.粉煤灰中重金属Pb/Cr/Cu在土壤—小白菜中的迁移与形态转化[J].土壤学报,2019,56(3):682-692.Bai L Y,Ji H H,Sun W X,et al.Morphological transformation and migration of heavy metals (Pb,Cr and Cu) in coal ash applied to the soil-Chinese cabbage system[J].Acta Pedologica Sinica,2019,56(3):682-692.
[24] Cao Y,Zhang B R,Guo M R,et al.Long-term stabilization of lead contaminated soil with a novel dithiocarboxylate functionalized hyperbranched polymer[J].Journal of Environmental Chemical Engineering,2022,10(5):108214.
[25] Liu S J,Liu Y G,Tan X F,et al.The effect of several activated biochars on Cd immobilization and microbial community composition during in-situ remediation of heavy metal contaminated sediment[J].Chemosphere,2018,208:655-664.
[26] Miao W,Cheng X Y,Meng J,et al.Ageing of rice husk biochar along a freeze-thaw cycles[J].MATEC Web of Conferences,2016,63:04013.
[27] 陈丙彤.改性抗生素菌渣水热炭Pb²⁺、Cd²⁺吸附性能及实验研究[D].济南:齐鲁工业大学,2023.Chen B T.Adsorption performance and experimental Study of modified antibiotic mycelium residue hydrothermal carbon for Pb²⁺ and Cd²⁺[D].Jinan:Qilu University of Technology,2023.
[28] Verheijen A G F,Graber R E,Ameloot N,et al.Biochars in soils:new insights and emerging research needs[J].European Journal of Soil Science,2014,65(1):22-27.
[29] Cui H B,Li D T,Liu X S,et al.Dry-wet and freeze-thaw aging activate endogenous copper and cadmium in biochar[J].Journal of Cleaner Production,2021,288:125605.
[30] Grüter R,Costerousse B,Mayer J,et al.Long-term organic matter application reduces cadmium but not zinc concentrations in wheat[J].Science of the Total Environment,2019,669:608-620.
[31] Ma Y,Cheng L,Zhang D D,et al.Stabilization of Pb,Cd,and Zn in soil by modified-zeolite:Mechanisms and evaluation of effectiveness[J].Science of the Total Environment,2022,814:152746.
[32] Xu Z B,Xu X Y,Tsang C D,et al.Contrasting impacts of pre-and post-application aging of biochar on the immobilization of Cd in contaminated soils[J].Environmental Pollution,2018,242(Pt B):1362-1370.
[33] Zhang P Y,Fan J,Xu X Y,et al.Contrasting effects of dry-wet and freeze-thaw aging on the immobilization of As in As-contaminated soils amended by zero-valent iron-embedded biochar[J].Journal of Hazardous Materials,2022,426:128123-128123.
[34] 张敏,郜春花,李建华,等.一株耐铅土著微生物的吸附特性及机制研究[J].山西农业科学,2018,46(8):1321-1328.Zhang M,Gao C H,Li J H,et al.Adsorption characteristics and mechanism of a lead-tolerant indigenous microorganism[J].Shanxi Agricultural Science,2018,46(8):1321-1328.
[35] 郜雅静,李建华,靳东升,等.耐铅菌与生物炭、有机肥配施对铅污染土壤的修复效果[J].山西农业科学,2019,47(11):1988-1994.Gao Y J,Li J H,Jin D S,et al.Remediation effect of lead-tolerant bacteria combined with biochar and organic fertilizer on lead-contaminated Soil[J].Shanxi Agricultural Science,2019,47(11):1988-1994.
[36] Sungur A,Soylak M,Yilmaz E,et al.Characterization of heavy metal fractions in agricultural soils by sequential extraction procedure:the relationship between soil properties and heavy metal fractions[J].Soil and Sediment Contamination An International Journal,2014,24(1):1-15.
[37] 周晓丽,包丽君,柳旭,等.干湿交替水分胁迫对水稻土细菌群落影响的研究[J].微生物学报,2022,62(3):1004-1019.Zhou X L,Bao L J,Liu X,et al.Research on the impact of wet-bry alternation water stress on bacterial communities in paddy soil[J].Acta Microbiologica Sinica,2022,62(3):1004-1019.
[38] 谢志煌,高志颖,郭丽丽,等.土壤微生物活性和生物量对干湿交替的响应[J].土壤与作物,2020,9(4):348-354.Xie Z H,Gao Z Y,Guo L L,et al.Response of soil microbial activity and biomass to bry-wet alternation[J].Soils and Crops,2020,9(4):348-354.
[39] Feng X J,Nielsen L L,Simpson J M.Responses of soil organic matter and microorganisms to freeze-thaw cycles[J].Soil Biology&Biochemistry,2007,39(8):2027-2037.
[40] Long X X,Yu Z N,Liu S W,et al.A systematic review of biochar aging and the potential eco-environmental risk in heavy metal contaminated soi l[J].Journal of Hazardous Materials,2024,472:134345.
[41] Wang Z,Bian Y,Xu Y L,et al.Artificial aging induced changes in biochar,s properties and Cd²⁺ adsorption behaviors[J].Environmental Science and Pollution Research International,2022,30:20133-20146.
[42] Ren X H,Sun H W,Wang F,et al.Effect of aging in field soil on biochar's properties and its sorption capacity[J].Environmental Pollution,2018,242(Pt B):1880-1886.
[43] Cao Y,Jing Y,Hao H,et al.Changes in the physicochemical characteristics of peanut straw biochar after freeze-thaw and dry-wet aging treatments of the biomass[J].BioResources,2019,14(2):4329-4343.
[44] 张敏.耐铅菌株的筛选及其吸附特征的研究[D].太原:山西大学,2018.Zhang M.Screening of lead-tolerant strains and their adsorption characteristics[D].Taiyuan:Shanxi University,2018.
[45] 王泽煌,王蒙,蔡昆争,等.细菌对重金属吸附和解毒机制的研究进展[J].生物技术通报,2016,32(12):1-18.Wang Z H,Wang M,Cai K Z,et al.Research progress on the adsorption and detoxification mechanisms of heavy metals by bacteria[J].Biotechnology Bulletin,2016,32(12):1-18.
[46] Chang R H,Sohi P S,Jing F,et al.A comparative study on biochar properties and Cd adsorption behavior under effects of ageing processes of leaching,acidification and oxidation[J].Environmental Pollution,2019,254(PB):113123.
[47] 吴丹萍,陈全,李东梅,等.生物炭含氧官能团的生成溯源及其在污染物吸附-降解过程中的作用[J].环境化学,2021,40(10):3190-3198.Wu D P,Chen Q,Li D M,et al.Formation source of oxygen-containing functional groups in biochar and their role in the adsorption-degradation process of pollutants[J].Environmental Chemistry,2021,40(10):3190-3198.
[48] Zhou X R,Zhu Y,Niu Q Y,et al.New notion of biochar:A review on the mechanism of biochar applications in advannced oxidation processes[J].Chemical Engineering Journal,2021,416:129027.
[49] 高瑞丽,唐茂,付庆灵,等.生物炭、蒙脱石及其混合添加对复合污染土壤中重金属形态的影响[J].环境科学,2017,38(1):361-367.Gao R L,Tang M,Fu Q L,et al.Fractions of heavy metals in contaminated soil after the addition of biochar,montmorillonite,and their mixture[J].Environmental Science,2017,38(1):361-367.
[50] Xu X Y,Cao X D,Zhao L.Comparison of rice husk-and dairy manure-derived biochars for simultaneously removing heavy metals from aqueous solutions:Role of mineral components in biochars[J].Chemosphere,2013,92(8):955-961.
[51] Zhang H Y,Yue X P,Li F,et al.Preparation of rice straw-derived biochar for efficient cadmium removal by modification of oxygen-containing functional groups[J].Science of the Total Environment,2018,631-632:795-802.
[52] 吴宇茜,韩琳希,钱敏,等.化学老化对Zn改性生物炭性质及吸附Pb²⁺的影响[J].中国环境科学,2024,44(2):803-813.Wu Y Q,Han L X,Qian M,et al.Effects of chemical aging on the properties of Zn-modified biochars and the adsorption properties of Pb²⁺[J].China Environmental Science,2024,44(2):803-813.