Effects of drought duration on the denitrification of biofilms in intermittent river
MIAO Ling-zhan1,2, LI Wei-yu1
1. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China; 2. College of Environment, Hohai University, Nanjing 210098, China
Abstract:This study took the biofilms cultivated in artificially simulated rivers as the test subjects, and explored the dynamic response of biofilms denitrification to rewetting process after short-term drought (25days) and long-term drought (90days). The results showed that the water content and macro-microscopic morphology of biofilm changed significantly during both drought and rewetting, and the water content dropped sharply in the 3d to 7d, from (74.96±3.57)% to (2.38±0.37)%. The denitrification rate of both short and long term drought groups showed a continuous increase after rewetting. The short-term drought group showed a significant increase (P<0.05), from (3.26±0.12) to (5.35±1.18)μmol N2-15N/(L·h), while the long-term drought group was concentrated in the late rewetting period, from (2.14±0.19) to (4.15±0.89)μmol N2-15N/(L·h) (P <0.05). Biofilms that experienced short-term drought were able to recover to the initial denitrification rate level through the rewetting process, while those that experienced long-term drought had difficulty achieving it (P<0.05), indicating that long-term drought had an irreversible effect on the denitrification rate of biofilm. The activities of denitrification functional enzymes also showed different changes during each rehydration stage. Short-term drought significantly increased the nitric oxide reductase activity at the end of the reaction pathway (P<0.05). While the nitrite reductase and nitrite oxide reductase, which directly affect the denitrification rate, also had difficulty in recovering to their initial levels after experiencing long-term drought (P<0.05). The above results show that prolonged drought significantly alters the biogeochemical cycle driven by river biofilms (denitrification function).
苗令占, 李玮钰. 脱水时长对间歇性河流生物膜反硝化功能的影响[J]. 中国环境科学, 2023, 43(8): 3918-3925.
MIAO Ling-zhan, LI Wei-yu. Effects of drought duration on the denitrification of biofilms in intermittent river. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(8): 3918-3925.
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