Alleviating cadmium toxicity to liver and kidney of mice with dietary supplementation of selenium and calcium in combination
WU Yang-yang1, PENG Xiang-wen1, SHI Ke-xin1, KUANG Wen-jie1, SUN Hong2, HUANG Lei1
1. State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210023, China; 2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210023, China
Abstract：To explore the mitigation role of dietary selenium (Se) and calcium (Ca) supplementation in combination in alleviating the biological toxicity of Cd, an in vivo mouse bioassay was performed. Mice (BALB/c, n = 88) that were exposed to Cd via diets at the same concentration of 2mg/kg were randomly divided to 19groups of dietary supplementation of low, medium, and high concentrations of Se and Ca in diets alone or in combination. The growth and development of mice, liver and kidney function, oxidative stress state and histopathological changes of the mice were measured after 30days of intervention. Results showed that the combined intervention of Se-Ca could effectively alleviate the growth retardation of mice caused by Cd exposure. In terms of liver and kidney function and oxidative stress, the combined intervention had a stronger protective effect on AST, GSH, SOD, GSH-Px in the liver and BUN, MDA and SOD in the kidney of mice than single Se or Ca intervention. Additionally, a lower dietary concentration of Se and Ca in combination alleviated pathological damage at a similar extent to that of a high concentration of Se or Ca alone. In general, Se-Ca dietary intervention in combination effectively alleviated the growth retardation and liver and kidney toxicity of mice caused by Cd exposure, and the effect was stronger than that of single element intervention.
吴杨洋, 彭祥雯, 史可欣, 匡雯洁, 孙宏, 黄蕾. Se-Ca联合膳食干预缓解Cd诱导小鼠肝肾毒性[J]. 中国环境科学, 2022, 42(3): 1379-1384.
WU Yang-yang, PENG Xiang-wen, SHI Ke-xin, KUANG Wen-jie, SUN Hong, HUANG Lei. Alleviating cadmium toxicity to liver and kidney of mice with dietary supplementation of selenium and calcium in combination. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1379-1384.
Zhao F J, Ma Y, Zhu Y G, et al. Soil Contamination in China:Current Status and Mitigation Strategies[J]. Environmental Science& Technology, 2015,49(2):750-759.
[2]
环境保护部.全国土壤污染状况调查公报[EB/OL]. http://www.gov.cn/foot/2014-04/17/content_2661768.htm, 2014-04-17. Ministry of environmental protection of the people's Republic of China. The Report on the national general survey of soil contamination[EB/OL]. http://www.gov.cn/foot/2014-04/17/content_2661768.htm, 2014-04-17.
[3]
蒋玉艳,马宁,蒙浩洋,等.广西居民重金属镉膳食摄入水平及其健康风险评估[J].中国食品卫生杂志, 2021,33(2):191-195. Jiang Y Y, Ma N, Meng H Y, et al. Risk assessment on the dietary exposure of cadmium in Guangxi residents[J]. Chinese Journal of Food Hygiene, 2021,33(2):191-195.
[4]
Anetor J I. Rising environmental cadmium levels in developing countries:Threat to genome stability and health[J]. Journal of Environmental Analytical Toxicology, 2012,27(2):103-115.
[5]
Rani A, Kumar A, Lal A, et al. Cellular mechanisms of cadmium-induced toxicity:a review[J]. International Journal of Environmental Health Research, 2014,24(4):378-399.
[6]
Ognjanovi B I, Markovi S D, Pavlovi S Z, et al. Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats:protective effect of selenium[J]. Physiological Research, 2008, 57(3):403-411.
[7]
Messner B, Bernhard D. Cadmium and cardiovascular diseases:cell biology, pathophysiology, and epidemiological relevance[J]. Biometals, 2010,23(5):811-822.
[8]
Huff J, Lunn R M, Waalkes M P, et al. Cadmium-induced cancers in animals and in humans[J]. International Journal of Occupational& Environmental Health, 2007,13(2):202-212.
[9]
Reeves P G, Chaney R L. Marginal nutritional status of zinc, iron, and calcium increases cadmium retention in the duodenum and other organs of rats fed rice-based diets[J]. Environmental Research, 2004, 96(3):311-322.
[10]
El-Boshy M E, Risha E F, Abdelhamid F M, et al. Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats[J]. Journal of Trace Elements in Medicine& Biology, 2015,29:104-110.
[11]
GB 2762-2017食品安全国家标准食品中污染物限量[S]. GB 2762-2017 China food safety national standard for maximum levels of contaminants in foods[S].
[12]
Su Y, Li L, Farooq M U, et al. Rescue effects of Se-enriched rice on physiological and biochemical characteristics in cadmium poisoning mice[J]. Environmental Science and Pollution Research, 2021,28(16):20023-20033.
[13]
Fang J, Yin H, Yang Z Z, et al. Vitamin E protects against cadmium-induced sub-chronic liver injury associated with the inhibition of oxidative stress and activation of Nrf2 pathway[J]. Ecotoxicology and Environmental Safety, 2021,208:111610.
[14]
Ge J, Liu L, Cui Z, et al. Comparative study on protective effect of different selenium sources against cadmium-induced nephrotoxicity via regulating the transcriptions of selenoproteome[J]. Ecotoxicology and Environmental Safety, 2021,215:112135.
[15]
Chen Z F, Shi K X, Kuang W J, et al. Exploration of the optimal strategy for dietary calcium intervention against the toxicity of liver and kidney induced by cadmium in mice:An in vivo diet intervention study[J]. PLoS One, 2021,16(5):e0250885.
[16]
Wang Y, Wu Y, Luo K, et al. The protective effects of selenium on cadmium-induced oxidative stress and apoptosis via mitochondria pathway in mice kidney[J]. Food and Chemical Toxicology, 2013,58:61-67.
[17]
Olisekodiaka M J, Igbeneghu C A, Onuegbu A J, et al. Total antioxidant status and organ changes in rats administered high doses of cadmium chloride[J]. Medical Principles and Practice, 2012,21(2):156-159.
[18]
Prabu S M, Shagirtha K, Renugadevi J. Quercetin in combination with vitamins (C and E) improve oxidative stress and hepatic injury in cadmium intoxicated rats[J]. Biomedicine& Preventive Nutrition, 2011,1(1):1-7.
[19]
Berglund M, Kesson A, Nermell B, et al. Intestinal absorption of dietary cadmium in women depends on body iron stores and fiber intake[J]. Environmental Health Perspectives, 1994,102(12):1058-1066.
