超富集植物青葙根际耐镉、铅、锌菌株的筛选鉴定与特性研究

王桉祺; 林华; 刘杰; 林毅; 杨雪萌; 赖才星; 董梓涵; 俞果

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中国环境科学 ›› 2025, Vol. 45 ›› Issue (5) : 2631-2642.

土壤污染与控制

超富集植物青葙根际耐镉、铅、锌菌株的筛选鉴定与特性研究

王桉祺¹, 林华^1,2,3, 刘杰^1,2, 林毅¹, 杨雪萌¹, 赖才星¹, 董梓涵¹, 俞果^1,4

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Isolation, identification, and characterization of a strain resistant to cadmium, lead, and zinc from the rhizosphere of hyperaccumulator Celosia argentea Linn

WANG An-qi¹, LIN Hua^1,2,3, LIU Jie^1,2, LIN Yi¹, YANG Xue-meng¹, LAI Cai-xing¹, DONG Zi-han¹, YU Guo^1,4

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摘要

为探究超富集植物根际促生菌的功能特性,从镉超富集植物青葙根际土壤中分离出一株能同时耐高浓度Cd²⁺、Pb²⁺和Zn²⁺的菌株,通过生理生化特性分析、16SrDNA及nrdA功能基因序列分析鉴定其种属,探究了不同培养条件对菌株耐受和去除重金属能力的影响,并评估了该菌株潜在的促生特性.该菌株被鉴定为无色杆菌属(Achromobactersp.),命名为WL-37.Cd²⁺、Pb²⁺和Zn²⁺对WL-37的最低抑制浓度分别为600,1800和1000mg/L.通过优化pH值、接种量等条件,发现WL-37对Cd²⁺、Pb²⁺和Zn²⁺的最佳去除效率分别达到69%、95%和62%.WL-37还具备固氮、产ACC脱氨酶和产铁载体等多种促生能力.筛选得到的高效功能菌株为重金属复合污染土壤修复提供了良好的菌种资源,为发展植物-微生物联合修复技术提供了技术支撑.

Abstract

This study investigated the functional characteristics of plant growth-promoting rhizobacteria (PGPR) isolated from the rhizosphere soil of Celosia argentea Linn., a Cd-hyperaccumulator. A strain with high tolerance to Cd^2-, Pb^2-, and Zn^2- was isolated. This strain was identified using physiological and biochemical characteristics analysis and sequence analysis of the 16S rDNA and nrdA functional genes. The effects of various culture conditions on the strain's growth and heavy metal removal capabilities, as well as its potential for promoting plant growth were examined. This strain was identified as Achromobacter sp., designated WL-37. The minimum inhibitory concentrations (MIC) of Cd^2-, Pb^2-, and Zn^2- for strain WL-37 were determined to be 600, 1800, and 1000mg/L, respectively. Under optimized conditions (e.g., pH values and inoculation amounts), the strain achieved maximum removal rates of 69% for Cd²⁺, 95% for Pb²⁺, and 62% for Zn²⁺. Moreover, WL-37 exhibited multiple plant-promoting traits, including nitrogen fixation, ACC deaminase production, and siderophore production. In summary, the high-efficiency strain identified in this study represents a valuable resource for the remediation of multi-metal contaminated soils and supports the development of plant-microbe combined remediation technologies.

导出引用

王桉祺, 林华, 刘杰, 林毅, 杨雪萌, 赖才星, 董梓涵, 俞果. 超富集植物青葙根际耐镉、铅、锌菌株的筛选鉴定与特性研究[J]. 中国环境科学. 2025, 45(5): 2631-2642

WANG An-qi, LIN Hua, LIU Jie, LIN Yi, YANG Xue-meng, LAI Cai-xing, DONG Zi-han, YU Guo. Isolation, identification, and characterization of a strain resistant to cadmium, lead, and zinc from the rhizosphere of hyperaccumulator Celosia argentea Linn[J]. China Environmental Science. 2025, 45(5): 2631-2642

中图分类号： X173

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