Vertical distribution of bacterial communities in sediments of Longjing Lake
NIU Feng-xia, JI Fang-ying, ZHAO Gen, ZHANG Qian, SHEN Qiu-shi, HE Qiang, YAN Hai-bo
Key Laboratory of Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing 400045, China
This study explored the vertical distribution of bacterial communities in sediments under different deposition time and its role in the migration and transformation of substances in lake ecosystem. Stratified sediment samples were collected respectively from Original River (OR) and New-formed Bay (NB) in Longjing Lake. Redundancy analysis (RDA) were conducted based on Denaturing Gradient Gel Electrophoresis (DGGE) technology and environmental parameters. Results showed that Shannon-Wiener indexes (H) and Richness indexes (S) in OR were higher than those in NB and vertical change trend of H, S and Evenness (E) indexes decreased first and then increased as depth increased. Phylogenetic analysis revealed that Longjing lake mainly included seven phyla of bacteria. Deltaproteobacteria, Bacteroidetes and Chloroflexi dominated in surface layers whereas Chloroflexi dominated in middle layers of OR sediments. Deltaproteobacteria and Chloroflexi were the main phyla in deep layers of both sites. Other phyla included Ignavibacteriae, Actinobacteria, Firmicutes and Chlorobi. RDA showed that the main factors influencing bacterial community composition and distribution were total nitrogen (TN), total sulphur (TS) and the concentrations of total organic carbon (TOC) in pore water, as well as average particle size in sediments. Otherwise, the obtained bacteria were closely related to the degradation of organic matter (OM). That TOC and TN contents in OR were higher than those in NB caused relatively higher bacteria species and abundance of Deltaproteobacteria, Bacteroidetes, and Ignavibacteriae.
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