Abstract：In this study, the impact of different sludge conditioning methods (polyacrylamide conditioning, Fe[III]/CaO conditioning, chemical acidification and bioleaching conditioning) on the streptomycin, gentamicin and kanamycin ARB in dewatered sludge cake and dewatering filtrate was investigated using heterotrophic plate counts. The results showed that PAM conditioning could not reduce the ARB in dewatered sludge cake and dewatering filtrate. Fe[III]/CaO conditioning and bioleaching conditioning not only reduced the amount of three kinds of ARB in dewatered sludge cake, but also completely eliminated the three ARB in dewatering filtrate. The chemical acidification conditioning reduced the ARB in dewatered sludge cake by 0.83~1.65 log units, but increased the ARB in dewatering filtrate by 0.26~0.39 log units. The removal of ARB in dewatered sludge cake significantly correlated with the degree of microbial cell destruction in conditioned sludge, implying that the destruction of microbial cells resulting from conditioning treatments was the main reason for the removal of ARB in dewatered sludge. Further study revealed that the amount of ARB rebounded during the natural storage of dewatered sludge cakes, which showed a certain environmental risk.
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