1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China; 2. Jiangsu Key Laboratory of Environmental Science and Engineering, Suzhou 215009, China; 3. Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215009, China
Abstract：In order to clarify the differences of the nitrogen and carbon removal functional bacteria community structure and metabolic pathway in the anammox granular sludge system with different concentrations of organic matter(50~150 mg/L) and bamboo biochar, the microbial distribution and gene expression of carbon and nitrogen metabolism were studied by metagenomic sequencing in this paper. The results showed that the bamboo charcoal addition was significantly increased the relative abundance of anammox when the COD concentration was 50 and 150 mg/L, the relative abundance of Candidatus_Kuenenia increased from 2.0%and 1.8% to 2.9% and 4.5%. In addition, compared with the control group only with organic matter(without bamboo biochar), the bamboo biochar addition was significantly changed the microbial community structure. The abundance of DNRA(dissimilatory nitrate reduction to ammonium process) bacteria was decreased, while the abundance of denitrifying bacteria and carbon metabolism-related bacteria were increased, which indicated that bamboo biochar addition was helpful to maintain the stability of nitrogen metabolism pathway community structure of anammox, denitrification and DNRA. The network analysis of microorganisms was showed that the removal efficiency of TN was increased by the synergetic effect of different nitrogen removal bacteria groups.However, the resistance of Candidatus_Brocadia and Candidatus_Jettenia could be improved by Halomonas enrichment with bamboo biochar addition. The KEGG annotation indicated that bamboo charcoal addition would be enhanced the carbon and nitrogen metabolism efficiency of anammox granular sludge system in the presence of organic matter, especially promoted the connection between EMP(glycolytic pathway) and TCA(tricarboxylic acid cycle).
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