Abstract：In order to explore the tolerance of biofilm treatment system to Nano-ZnO, a sequencing batch biofilm reactor (SBBR) was constructed to carry out the Nano-ZnO stress test in SBBR system for determining the accumulation characteristics of Nano-ZnO on biofilm, investigating the removal performance of organic matter, nitrogen and phosphorus, and evaluating the Nano-ZnO tolerance threshold of SBBR. The responses of microbial community to Nano-ZnO were also analyzed by observing the changes of biomass, microbial activity and community structure. The results showed that low concentration (1~10mg/L) Nano-ZnO had no significant effect on the removal of COD, NH4+-N and SOP in SBBR except that 5mg/L Nano-ZnO promoted the microbial metabolic rate and biological activity. While the concentration of Nano-ZnO increased to 50mg/L, the inhibition on biomass and microbial activity increased, and the removal of COD, NH4+-N and SOP decreased by 26.45%, 57.83% and 43.50%, respectively. Nano-ZnO stress had the least effect on COD removal performance, but a great effect on NH4+-N. The Nano-ZnO tolerance threshold of SBBR indicated by COD was 911.49mg, while that of NH4+-N and SOP was 579.83mg. The diversity of microbial community in SBBR was reduced and the composition of community structure were changed under the stress of Nano-ZnO. The relative abundance of Proteobacteria and Chlorofiexi decreased from 21.09% and 7.03% to 8.00% and 2.60%, respectively, resulting in significant inhibition of NH4+-N removal. The abundance of Paesciabacteria suddenly increased from 9.33% to 56.64%, which played an important role in the efficient removal of organic matter. The pollutant removal performance and microbial activity showed that the tolerance of SBBR biofilm system to Nano-ZnO was stronger than that of activated sludge process.
高静湉, 胡鹏, 蔡怡婷, 李卫平, 刘超. 纳米ZnO胁迫下SBBR污染物去除性能及微生物群落响应[J]. 中国环境科学, 2022, 42(8): 3658-3665.
GAO Jing-tian, HU Peng, CAI Yi-ting, LI Wei-ping, LIU Chao. Performance of pollutant removal and responses of microbial community to Nano-ZnO stress in SBBR. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(8): 3658-3665.
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