Cardiotoxicity and oxidative damage of phosalone exposure on zebrafish embryos
SHENG Zhong, CHEN Jia-le, WANG Bin-jie, WU Yuan-zhao, YAO Wei-xuan, WANG Ji-ye
Key Laboratory of Drug Prevention and Control Technology Research in Zhejiang Province, Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China
Abstract：The effects of acute exposure to sublethal doses of phosalone on the development of zebrafish embryos were evaluated in terms of cardiotoxicity and oxidative damage. The results showed that the hatching rate of zebrafish embryos decreased by 10.37% and 22.96% at 1and 3mg/L, respectively, and the body length shortened by 11.77% and 19.40%, respectively, and the heart rate decreased significantly, resulting in embryonic developmental abnormalities such as pericardial edema. Superoxide dismutase (SOD) activity significantly increased after exposure to phoslone at concentrations of 0.3mg/L and 1mg/L, but decreased in the 3mg/L group;catalase (CAT) activity decreased significantly in zebrafish larvae; the reactive oxygen species (ROS) assay showed that the heart of zebrafish was the main organ responsible for oxidative damage; transgenic zebrafish (cmlc2:EGFP) showed increased separation of atria and ventricles; the expression of nppa and sox9b were significantly up-regulated and gata4 and vmhc were significantly down-regulated in heart development-related genes; the expression of apoptosis-related genes bcl2, bax, Puma and Mdm2 were significantly down-regulated. The result suggested that the cardiotoxicity induced by phosalone exposure was associated with oxidative damage and disruption of apoptotic cell homeostasis.
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