Structure and assembly processes of bacterial communities in Poyang Lake basin
SHE Yuan-yang1,2, WANG Peng1, DING Ming-jun1, NIE Ming-hua1, HUANG Gao-xiang1, ZHANG Hua1, HUANG Yi-ping1, CAO Ying-jie3
1. Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, School of Geography and Environment, Jiangxi Normal University, Nanchang 330022, China; 2. School of History Culture and Tourism, Longnan Teachers College, Longnan 742500, China; 3. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China
Abstract：To investigate the structure and assembly processes of bacterial communities across different spatial-temporal scales, a total of eight surface water samples were collected in Poyang Lake basin (from seven hydrological stations) during the dry season (October to January of the following year) and wet season (April to August), and high-throughput sequencing technology was utilized to examine the bacterial community's structure, assembly processes, and co-occurrence networks. The results demonstrated that the Shannon index was significantly higher (5.12) during the wet season compared to the dry season (4.45), while the Chao1index did not differ significantly between the seasons. Moreover, significant differences in a diversity were observed within the same season, while no significant differences were detected in the a and b diversity of bacterial communities in space. The dominant phyla in the Poyang Lake basin were Pseudomonadota (34.72%), Actinobacteria (32.35%), Cyanobacteria (13.21%), and Bacteroidetes (10.81%). The relative abundance of Pseudomonadota and Actinobacteriota decreased significantly, while Cyanobacteria and Bacteroidota significantly increased during the wet season compared to the dry season. Water temperature (WT) and mean stream network length (MDSL) were identified as the primary environmental factors influencing the structure of the bacterial community at the phyla-level. The co-occurrence networks of bacterial communities in water bodies interacted with each other in collaborative and competitive relationships, which were stronger, more complex, and stable during the wet season than in the dry season. Additionally, the complexity and stability of the co-network were significantly influenced by both WT and dissolved oxygen (DO). The assembly of bacterial communities was predominantly dominated by deterministic processes, with a stronger contribution from these processes during the dry season than in the wet season. Finally, some differences in co-network patterns and assembly processes were observed across different time scales.
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