Research on the performance and microbial community of a one-stage partial nitritation-anammox reactor at room temperature
LI Yun-fei1, WANG Si-ke1,2, ZUO Jian-e1
1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; 2. Shenzhen Polytechnic, Shenzhen 518055, China
Abstract：Partial nitritation-anammox (PNA) is an emerging technology for autotrophic nitrogen removal. During the application, it usually faces the hurdle of temperature fluctuation, especially temperature decrease during winter. In this regard, a lab scale one-stage PNA reactor was operated with a decreased temperature from 32℃ to 22℃ to investigate its effect on the system performance, microbial activity and community structure. It turned out that, during the whole experiment with influent ammonia-N concentration of about 254mg/L, the nitrogen loading rate and removal rate were in the range of 1.0~1.2g/(L·d) and 0.7~0.9g/(L·d), respectively. The total nitrogen removal efficiency achieved 62%~88%, and the effluent nitrite remained at a low level of <10mg/L, while the effluent nitrate concentration increased along with the decrease of temperature. Decreased temperature had an adverse effect on the microbial activity of anammox bacteria (AnAOB) and ammonia oxidized bacteria (AOB), while had a positive effect on that of nitrite oxidized bacteria (NOB). However, it should be pointed out that the AOB always showed a higher microbial activity, followed by AnAOB, and the activity of NOB was at a significantly low level. 22℃ was a critical temperature for the change of AnAOB activity, which should be paid more attention during operation. In the PNA system, Ca.Brocadia and Nitrosomonas were found to be the most abundant AnAOB and AOB with respective relative abundance of 2.7%~15.1% and 2.8%~11.5%. It should be noted that the dominant genus of AnAOB switched to Ca. Brocadia from Ca. Jettenia at the initial stage, implying that the former possessed more competitive advantages at low temperature.
李云飞, 王丝可, 左剑恶. 一体式厌氧氨氧化反应器常温运行与微生物群落研究[J]. 中国环境科学, 2021, 41(3): 1172-1180.
LI Yun-fei, WANG Si-ke, ZUO Jian-e. Research on the performance and microbial community of a one-stage partial nitritation-anammox reactor at room temperature. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(3): 1172-1180.
中华人民共和国环境保护部.2015中国环境统计年报[R]. 中国环境出版社, 2016. Ministry of Environmental Protection of the People's Republic of China. 2015Annual statistic report on environment in China[R]. China Environmental Science Press, 2016.
[2]
中华人民共和国生态环境部.2018年中国生态环境状况公报[R]. 北京:2019. Ministry of Ecology and Environment of the People's Republic of China. 2018 China Ecology and Environment Statement[R]. 2019. Beijing.
[3]
Lackner S, Gilbert E M, Vlaeminck S E, et al. Full-scale partial nitritation/anammox experiences——an application survey[J]. Water Research, 2014,55:292-303.
[4]
Li J, Li J, Gao R, et al. A critical review of one-stage anammox processes for treating industrial wastewater:Optimization strategies based on key functional microorganisms[J]. Bioresource Technology, 2018,265:498-505.
[5]
Nsenga Kumwimba M, Lotti T, Şenel E, et al. Anammox-based processes:How far have we come and what work remains? A review by bibliometric analysis[J]. Chemosphere, 2020,238:124627.
[6]
杨延栋,黄京,韩晓宇,等.一体式厌氧氨氧化工艺处理高氨氮污泥消化液的启动[J]. 中国环境科学, 2015,35(4):1082-1087. Yang Y D, Huang J, Han X Y, et al. Start-up of one-stage partial nitrification/anammox process treating ammonium-rich reject water[J]. China Environmental Science, 2015,35(4):1082-1087.
[7]
Tang C, Zheng P, Wang C, et al. Performance of high-loaded ANAMMOX UASB reactors containing granular sludge[J]. Water Research, 2011,45:135-144.
[8]
Yue X, Yu G, Lu Y, et al. Effect of dissolved oxygen on nitrogen removal and the microbial community of the completely autotrophic nitrogen removal over nitrite process in a submerged aerated biological filter[J]. Bioresource Technology, 2018,254:67-74.
[9]
程军,张亮,张树军,等.氨氮负荷波动对城市污水短程硝化-厌氧氨氧化工艺硝态氮的影响[J]. 中国环境科学, 2017,37(2):520-525. Cheng J, Zhang L, Zhang S J, et al. The effects of ammonium loading rate fluctuation on nitrate accumulation in municipal wastewater partial nitritation/anammox (PN/A) process[J]. China Environmental Science, 2017,37(2):520-525.
[10]
Pérez J, Lotti T, Kleerebezem R, et al. Outcompeting nitrite-oxidizing bacteria in single-stage nitrogen removal in sewage treatment plants:A model-based study[J]. Water Research, 2014,66:208-218.
[11]
Poot V, Hoekstra M, Geleijnse M a A, et al. Effects of the residual ammonium concentration on NOB repression during partial nitritation with granular sludge[J]. Water Research, 2016,106:518-530.
[12]
Gilbert E M, Agrawal S, Brunner F, et al. Response of different nitrospira species to anoxic periods depends on operational DO[J]. Environmental Science & Technology, 2014,48:2934-2941.
[13]
狄斐,隋倩雯,陈彦霖,等.部分亚硝化-厌氧氨氧化处理磁混凝生活污水[J]. 中国环境科学, 2020,40(11):4712-4720. Di F, Sui Q W, Chen Y L, et al. Partial nitritation-Anammox process treating magnetic coagulation domestic sewage[J]. China Environmental Science, 2020,40(11):4712-4720.
[14]
Cao Y, Hong K, Van Loosdrecht M C, et al. Mainstream partial nitritation and anammox in a 200,000m3/day activated sludge process in Singapore:scale-down by using laboratory fed-batch reactor[J]. Water Science Technology, 2016,74:48-56.
[15]
Lotti T, Kleerebezem R, Hu Z, et al. Simultaneous partial nitritation and anammox at low temperature with granular sludge[J]. Water Research, 2014,66:111-121.
[16]
Laureni M, Falas P, Robin O, et al. Mainstream partial nitritation and anammox:long-term process stability and effluent quality at low temperatures[J]. Water Research, 2016,101:628-639.
[17]
Lotti T, Kleerebezem R, Van Loosdrecht M C M. Effect of temperature change on anammox activity[J]. Biotechnology and Bioengineering, 2015,112:98-103.
[18]
杨朝晖,徐峥勇,曾光明,等.不同低温驯化策略下的厌氧氨氧化活性[J]. 中国环境科学, 2007,(3):300-305. Yang Z H, Xu Z Y, Zeng G M, et al. Anaerobic ammonia oxidation activity under different low temperature domestication strategy[J]. China Environmental Science, 2007,(3):300-305.
[19]
Gustavsson D J I, Suarez C, Wilen B M, et al. Long-term stability of partial nitritation-anammox for treatment of municipal wastewater in a moving bed biofilm reactor pilot system[J]. Science of The Total Environment, 2020,714:136342.
[20]
Massara T M, Malamis S, Guisasola A, et al. A review on nitrous oxide (N2O) emissions during biological nutrient removal from municipal wastewater and sludge reject water[J]. Science of The Total Environment, 2017,596-597:106-123.
[21]
国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法(第四版)[M]. 中国环境科学出版社, 2002. Editorial Board of Water and wastewater monitoring and analysis methods, State Environmental Protection Agency of China. Water and wastewater monitoring and analysis methods (Fourth Edition)[M]. China Environmental Science Press, 2002.
[22]
Vazquez-Padin J, Fernandez I, Morales N, et al. 2010. Autotrophic nitrogen removal at low temperature[M].
[23]
徐峥勇.基于亚硝化、厌氧氨氧化与反硝化的脱氮耦合工艺及其控制策略研究[D]. 长沙:湖南大学, 2011. Xu Z Y. Study on control strategies of partial nitrification, anaerobic ammonium oxidation and denitrification coupling process[D]. 2011. Changsha:Hunan University.
[24]
Akaboci T R V, Gich F, Ruscalleda M, et al. Assessment of operational conditions towards mainstream partial nitritation-anammox stability at moderate to low temperature:Reactor performance and bacterial community[J]. Chemical Engineering Journal, 2018,350:192-200.
[25]
王春香,刘常敬,郑林雪,等.厌氧氨氧化耦合脱氮系统中反硝化细菌研究[J]. 中国环境科学, 2014,34(7):1878-1883. Wang C X, Liu C J, Zheng L X, et al. Denitrifying bacteria of anaerobic ammonium-oxidizing denitrifying system[J]. China Environmental Science, 2014,34(7):1878-1883.
[26]
Hendrickx T L G, Kampman C, Zeeman G, et al. High specific activity for anammox bacteria enriched from activated sludge at 10℃[J]. Bioresource Technology, 2014,163:214-221.
[27]
Hu Z, Lotti T, De Kreuk M, et al. Nitrogen removal by a nitritation-anammox bioreactor at low temperature[J]. Applied and Environmental Microbiology, 2013,79:2807-2812.
