Spatiotemporal dynamics of microbial communities involved in soil carbon cycling of subalpine temperate coniferous forest
LI Cui1, QIAO Sha-sha2, LIU Jian-xian2, CHAI Bao-feng2
1. College of Resources and Environmental Sciences, Shanxi University of Finance and Economics, Taiyuan 030006, China; 2. Shanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China
Abstract：The cbbM, amylase and cellulase genes were selected as molecular markers to analyze functional microbial groups using real-time quantitative PCR method. We investigated the spatiotemporal dynamics of abundance of functional microbial groups in soils of four forests:Larix gmelinii var. principis rupprechtii, Picea meyeri, P. Wilsonii and Pinus tabulaeformis in subalpine temperate of north China. The results indicated that soil content of total carbon (TC), total nitrogen (TN), total sulfur (TS), organic matter (OM) and total organic carbon (TOC), pH, ammonium nitrogen (NH4+-N), nitrate nitrogen(NO3--N), and the activities of catalase, sucrase and urease were different in the soil of four forests, and exhibited seasonal dynamics variation. The contents of TC, TN, TS, C/N, OM and TOC were the highest, but pH value was the lowest in the higher altitude L. principis forest soil. The contents of TC, TN, nitrite nitrogen (NO2--N), sucrase activity and urease activity were significantly correlated with the abundance of microbial groups involving in carbon fixation, starch degradation and cellulose degradation. The content of NO3--N in soil was significantly related to the relative abundance of carbon decomposing and carbon fixing microorganisms. Soil C/N, NO2--N, OM, TOC content, pH, catalase and urease activity were significantly correlated with the relative abundance of microbial groups involving in degradation of labile carbon and recalcitrant carbon. Vegetation type and seasonal change jointly affected the abundance of soil functional microbial groups, but seasonal change was the dominant factor. The vegetation and soil environmental factors affected the carbon source-sink balance of forest soil by regulating the structure of carbon metabolism functional groups of microbial community.
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