低C/N亚铁氧化硝酸盐还原菌群脱氮特性

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摘要为削减微污染水库中氮污染负荷，从西安市李家河和黑河水库沉积物中驯化筛分出在低C/N条件下能够高效脱氮的亚铁氧化硝酸盐还原混合菌群，并将其命名为Z13.利用硝酸盐氮为唯一氮源、亚铁与乙酸作为共同电子供体的反硝化基础培养基，研究了亚铁氧化硝酸盐还原混合菌群Z13对氮素、亚铁和有机物等的代谢特征.考察了不同温度、初始pH值、C/N、Fe²⁺浓度对亚铁氧化硝酸盐还原混合菌群Z13脱氮性能及亚铁氧化的影响.结果表明，低C/N条件下亚铁氧化硝酸盐还原混合菌群Z13在78h时硝酸盐氮去除率为99.85%，总氮去除率为89.91%.Fe²⁺的总去除率为99.86%，其中生物氧化率为82.70%，无亚硝酸盐氮和氨氮的累积.单因素实验表明，在温度30℃，pH 6.5，C/N 1.821，Fe²⁺浓度30mg/L时，亚铁氧化硝酸盐还原混合菌群Z13对亚铁氧化和氮素的去除效果最好.亚铁氧化硝酸盐还原混合菌群Z13在微污染水体生物脱氮领域中具有很大的应用潜力.

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关键词 ：低C/N, 亚铁氧化, 硝酸盐还原, 生物脱氮

Abstract：In order to reduce the nitrogen pollution load in micro-polluted reservoirs, a mixture of ferrite nitrate reduction bacteria was isolated from sediments of Lijiahe and Heihe reservoirs in Xi'an and named as Z13, which can remove nitrogen efficiently under low C/N conditions. Using nitrate nitrogen as the only nitrogen source and iron and acetic acid as the common electron donor, the metabolic characteristics of mixed bacteria Z13 with iron nitrate reduction on nitrogen, iron and organic matter were studied. The effects of different temperatures, initial pH, C/N, and Fe²⁺concentrations on the denitrification performance and ferrous oxidation of mixed bacteria Z13 were investigated. The results showed that under low C/N condition, the ferrous oxidizing nitrate-reducing mixed bacterial group Z13 had a nitrate nitrogen removal rate of 99.85%, a total nitrogen removal rate of 89.91%, a total removal rate of Fe²⁺ of 99.86%, and the oxidation rate was 82.70% at 78hours. And there was no accumulation of nitrite nitrogen and ammonia nitrogen. The single factor experiment showed that when the temperature was 30℃, pH 6.5,C/N 1.821, and the concentration of Fe²⁺ was 30mg/L, the reduction of mixed bacteria Z13 by ferrous nitrate had the best removal effect on ferrous oxidation and nitrogen. The mixed bacteria Z13 reduced by ferrous oxide nitrate had great application potential in the field of biological nitrogen removal in slightly polluted water.

Key words： low C/N ferrous oxidation nitrate reduction biological denitrification

收稿日期: 2020-12-28

PACS:	X172
	X523

基金资助:陕西省自然科学基础研究计划项目（2019JLM-61）；陕西省重点研发计划项目（2018ZDXM-SF-029）

通讯作者: 黄廷林,教授,huangtinglin@xauat.edu.cn E-mail: huangtinglin@xauat.edu.cn

作者简介: 周佳敏(1996-),女,河南济源人,西安建筑科技大学硕士研究生,主要研究方向为水处理与微污染控制.

引用本文:

周佳敏, 黄廷林, 刘茜, 杨尚业, 寇莉青. 低C/N亚铁氧化硝酸盐还原菌群脱氮特性[J]. 中国环境科学, 2021, 41(8): 3723-3732. ZHOU Jia-min, HUANG Ting-lin, LIU Qian, YANG Shang-ye, KOU Li-qing. Denitrifying characteristics of low C/N ferrous oxidation nitrate reducing bacteria flora. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(8): 3723-3732.

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http://www.zghjkx.com.cn/CN/ 或 http://www.zghjkx.com.cn/CN/Y2021/V41/I8/3723

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