聚苯乙烯微塑料对菌-藻颗粒污泥的影响机制研究

徐航; 张冰; 时文歆

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中国环境科学 ›› 2023, Vol. 43 ›› Issue (10) : 5562-5573.

新污染物与环境毒理

聚苯乙烯微塑料对菌-藻颗粒污泥的影响机制研究

徐航, 张冰, 时文歆

作者信息 +

Unravelling the influencing mechanism of polystyrene microplastics on the algal-bacterial granular sludge systems

XU Hang, ZHANG Bing, SHI Wen-xin

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摘要

在成熟的好氧颗粒污泥（AGS）中接种小球藻，成功构建了菌-藻共生好氧颗粒污泥（ABGS）系统.开展了聚苯乙烯微塑料（PS-MPs）对ABGS系统的影响机制研究.研究表明：低浓度PS-MPs（1mg/L）对ABGS的生物量、藻类生长、沉降性能和污染物去除效率无明显影响.相较于ABGS对照组，高浓度PS-MPs（10mg/L和100mg/L）胁迫作用下ABGS的生物量、藻类生长、沉降性能受到了明显抑制，叶绿素α/MLSS比值降低了0.19mg/g，污泥平均粒径降低了200μm，颗粒污泥结构逐渐松散破碎，有机物、氮和磷等污染物的去除效率分别降低了4.83%、5.06%和4.11%.此外，PS-MPs抑制了ABGS胞外聚合物（EPS）的分泌，降低了EPS中色氨酸和芳香族蛋白质的含量.在微生物群落结构组成方面，PS-MPs提高了ABGS系统内物种多样性，但抑制了疣微菌门（Verrucomicrobiota）、蓝藻门（Cyanobacteria）、绿弯菌门（Chloroflexi）等功能细菌的生长，不利于维持系统的长期稳定性.

Abstract

The algal-bacterial granular sludge (ABGS) system was established by inoculating the mature aerobic granular sludge (AGS) and targeted algae (Chlorella). The effects of polystyrene microplastics (PS-MPs) on the ABGS system was investigated in this study. Results indicated that low concentrations of PS-MPs (1mg/L) had no significant effect on the biomass content, algal growth, sludge settling performance and pollutant removal efficiency of ABGS. Compared with the control group, the biomass content, algal growth and sludge settling performance of ABGS were inhibited under the pressure of high concentrations of PS-MPs (10mg/L and 100mg/L). To be specific, the ratio of chlorophyll α to MLSS decreased by 0.19mg/g, and the average particle size of sludge decreased by 200μm. Meanwhile, the granular sludge structure gradually became loosely and disintegrated. The removal efficiencies of chemical oxygen demand (COD), nitrogen, phosphorus of ABGS system were significantly decreased by 4.83%, 5.06% and 4.11%, respectively. In addition, PS-MPs inhibited the secretion of extracellular polymer substance (EPS) of ABGS, especially for the tryptophan and aromatic proteins. In terms of the microbial community structure, PS-MPs improved the diversity of bacterial species in the ABGS system, but inhibited the growth of functional bacteria (such as Verrucomicrobiota, Cyanobacteria and Chloroflexi), which were not conducive to maintaining the long-term stability of ABGS system.

导出引用

徐航, 张冰, 时文歆. 聚苯乙烯微塑料对菌-藻颗粒污泥的影响机制研究[J]. 中国环境科学. 2023, 43(10): 5562-5573

XU Hang, ZHANG Bing, SHI Wen-xin. Unravelling the influencing mechanism of polystyrene microplastics on the algal-bacterial granular sludge systems[J]. China Environmental Science. 2023, 43(10): 5562-5573

中图分类号： X703.1

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