The oxidative polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitated tumor-endothelial cell adhesion through histone acetylation
LI Dan1, XUE Mei-ying2
1. School of Materials Industry, Shanxi College of Technology, Shuozhou 036000, China; 2. Taiyuan Ecological and Environmental Monitoring and Scientific Research Center, Taiyuan 030002, China
Abstract：On the basis of analyzing the contents of three oxidative polycyclic aromatic hydrocarbons (Oxy-PAHs) in atmospheric PM2.5 samples during heating and non-heating periods in Taiyuan, one of them was selected for in vitro cell exposure. Western Blot and chromatin immunoprecipitation (ChIP) were used to investigate the adhesion of tumor-endothelial cells. The results showed that the content of 9-fluorenone was relatively stable, so 9-fluorenone was used for exposure. Tumor-endothelial cell adhesion was enhanced in a concentration-dependent manner, and the strongest was 3.24times that of control. Moreover, the levels of adhesion molecules E-selectin and VCAM-1 were significantly increased when the concentration of 9-fluorenone was 20 and 100μmol/L. When the concentration of 9-fluorenone was 20μmol/L, the expression of tumor cell adhesion molecule ICAM-1 was also significantly increased. In this process, the E-selectin binding to acetylated histone after 20μmol/L 9-fluorenone exposure was 5.97 times that of control. These results demonstrated a potential mechanism about Oxy-PAHs facilitate tumor metastasis by epigenetic regulation and suggested new research ideas for the treatment of lung cancer caused by Oxy-PAHs environmental exposure.
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