Impacts of PFOS exposure on signaling pathways in lung cancer cells
YU Zhi-hui1, DONG Jing-ying1, WANG Ya-nan2, WANG Xia-yan2
1. Department of Environmental Sciences, Beijing University of Technology, Beijing 100124, China; 2. Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Biology, Beijing University of Technology, Beijing 100124, China
Abstract：The effects of different concentrations of perfluorooctane sulfonate (PFOS) on the viability of A549 cells were determined by the CCK-8 method. The effects of PFOS exposure on miRNAs expression in A549 cells were detected by the next-generation sequencing method to investigate the molecular mechanism of pulmonary toxicity caused by PFOS. Target genes with abnormal expression of miRNAs were predicted, and their involved signaling pathways and potential biological functions were inferred through bioinformatics analysis. The results showed that a low concentration of PFOS (<200μmol/L) promoted the proliferation of A549 cells, while a high concentration of PFOS inhibited the proliferation of A549 cells. The expression levels of 108 miRNAs and 63 miRNAs in A549 cells exposed to 300 μmol/L PFOS for 24 h were significantly up-regulated and down-regulated. Differentially expressed miRNAs participate in biological processes such as cell proliferation, metabolic process, and developmental process through signaling pathways such as Ras, Rap1, HIF-1, ErbB, VEGF and so on. This study suggested that PFOS can threaten the lung by affecting cell proliferation and inducing inflammation.
于志辉, 董晶荧, 王亚楠, 汪夏燕. PFOS暴露对肺癌细胞中信号通路的影响[J]. 中国环境科学, 2021, 41(10): 4878-4884.
YU Zhi-hui, DONG Jing-ying, WANG Ya-nan, WANG Xia-yan. Impacts of PFOS exposure on signaling pathways in lung cancer cells. CHINA ENVIRONMENTAL SCIENCECE, 2021, 41(10): 4878-4884.
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