外源碳氮输入对沟道底泥反硝化潜势与阈值影响——以宁夏引黄灌区排水沟为研究对象

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摘要采集宁夏引黄灌区排水沟道底泥，开展上覆水土柱培养试验，分别模拟0，5，10，20mg/L 4种外源氮输入梯度和0，100mg/L 2种外源碳输入梯度处理下沟道水质的变化情况.在水力停留培养47d内对上覆水NO₃^--N、NH₄⁺-N、DOC及反硝化速率进行测定，并计算氮素消纳量以评估底泥反硝化脱氮潜势与阈值.结果表明：上覆水NO₃^--N浓度随时间延长而降低（P<0.01），且同时段内上覆水NO₃^--N浓度在外源碳添加较无碳源条件下显著降低（P<0.05），试验末期NO₃^--N浓度在无碳源和添加碳源条件下分别下降52.1%，93.6%；添加碳源条件下上覆水NO₃^--N浓度在20d时已稳定至较低水平，而未添加碳源处理NO₃^--N浓度在试验47d后仍有较大消纳空间；无碳源和添加碳源条件下底泥反硝化氮素消纳量阈值分别为263.7，865.6μmol/L，氮素累积消纳量占培养柱体系内总氮量的比率随外源碳氮的增加而增大（P<0.05），未添加碳源条件下反硝化过程可以消纳培养土柱体系内10.3%~11.4%的氮量，而在添加碳源后提高至17.7%~37.3%本研究可为引黄灌区农业面源污染高效治理提供科学依据.

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关键词 ：反硝化, 沟道脱氮, 外源碳氮, 氮素消纳

Abstract：In this study, laboratory incubation experiments were conducted for sediment denitrification potential study in farmland ditches of the Yellow River Diversion Irrigation Area of Ningxia. Experiments were conducted under condition of four exogenous nitrogen concentration gradients of 0, 5, 10, 20mg/L and two exogenous carbon concentration gradients of 0 and 100mg/L in the overlying water. The NO₃^--N, NH₄⁺-N, and DOC concentration of the overlying water were measured as well as denitrification rates during 47days of incubation to evaluate the denitrification potential and its threshold. The results showed that sediment denitrification increased with the increase of exogenous nitrogen and of carbon. The NO₃^--N concentration of the overlying water decreased with the time (P<0.01), but the concentration of NO₃^--N in the overlying water decreased significantly with the addition of exogenous carbon compared with the condition without carbon source (P<0.05). At the end of the experiment, NO₃^--N concentration decreased by 52.1% and 93.6% respectively without and with carbon sources. Under the condition of adding carbon source, the concentration of NO₃^--N in the overlying water stabilized to a low level at 20d, while the concentration of NO₃^--N in the treatment without carbon source addition still had a large decreasing space after 47d of the incubation. The thresholds of denitrification removal rate in sediment without carbon source and with carbon source were 263.7 and 865.6µmol/L, respectively. The ratio of cumulative nitrogen removal to total nitrogen in the culture column system increased with the increase of exogenous nitrogen and carbon source (P<0.05), denitrification process could remove 10.3%~11.4% of nitrogen in the culture column system without exogenous carbon, but increased to 17.7%~37.3% with the exogenous carbon addition. In conclusion, This study can provide scientific basis for efficient control of agricultural non-point source pollution in the irrigated area of Yellow River diversion.

Key words： denitrification channel denitrification exogenous carbon and nitrogen nitrogen removal

收稿日期: 2022-05-16

PACS:

X52

基金资助:国家自然科学基金资助项目（U20A20113，42177393）；宁夏回族自治区重点研发计划项目（2022CMG02007）

通讯作者: 佘冬立,教授,shedongli@hhu.edu.cn;夏永秋,研究员,yqxia@issas.ac.cn E-mail: shedongli@hhu.edu.cn;yqxia@issas.ac.cn

作者简介: 赵君涵(1998-),女,陕西咸阳人,硕士研究生,主要研究方向为农业水土过程模拟.发表论文1篇.

引用本文:

赵君涵, 佘冬立, 盖佳敏, 单军, 潘永春, 夏永秋, 洪瑜. 外源碳氮输入对沟道底泥反硝化潜势与阈值影响——以宁夏引黄灌区排水沟为研究对象[J]. 中国环境科学, 2022, 42(12): 5856-5863. ZHAO Jun-han, SHE Dong-li, GE Jia-min, SHAN Jun, PAN Yong-chun, XIA Yong-qiu, HONG Yu. Impact on denitrification potential and threshold of ditches sediment with exogenous carbon and nitrogen addition: A case of ditches in Ningxia Yellow River Irrigation area. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(12): 5856-5863.

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[1]	刘国强,杨世琦.宁夏引黄灌区农田退水污染现状分析[J]. 灌溉排水学报, 2010,29(1):104-108. Liu G Q, Yang S Q. Analyzed on present situation of subsiding water from cropland in Ningxia irrigation area from the Huanghe River[J]. Journal of Irrigation and Drainage, 2010,29(1):104-108.
[2]	白素颖.地下水适宜深度分期控制对沟道蓄水减污影响研究[D]. 扬州:扬州大学, 2016. Bai S Y. Reseach on the influence of stage control groundwater on ditch water storage and pollution reduction[D]. Yangzhou:Yangzhou University, 2016.
[3]	高雪梅,佘冬立,颜晓元,等.不同量碳源输入梯度下果园排水沟底泥氮素反硝化与N2O排放研究[J]. 环境科学, 2016,37(7):2731-2737. Gao X M, She D L, Yan X Y, et al. Denitrification loss and N2O emission from different carbon inputs in orchard drains sediments[J]. Environmental Science, 2016,37(7):2731-2737.
[4]	佘冬立,陈心逸,高雪梅,等.外源氮输入对不同土地利用排水沟底泥反硝化和N2O排放影响[J]. 环境科学, 2018,39(8):3689-3695. She D L, Chen X Y, Gao X M, et al. Impact of exogenous nitrogen import on sediment denitrification and N2O emissions in ditches under different land uses[J]. Environmental Science, 2018,39(8):3689-3695.
[5]	Li X B, Xia Y Q, Li Y F, et al. Sediment denitrification in waterways in a rice-paddy-dominated watershed in eastern China[J]. Journal of Soil & Sediments, 2013,13(4):783-792.
[6]	Zhang W, Li H, Kendall A D, et al. Nitrogen transport and retention in a headwater catchment with dense distributions of lowland ponds[J]. Science of the Total Environment, 2019,683:37-48.
[7]	吴军,崔远来,赵树君,等.沟塘湿地对农田面源污染的降解试验[J]. 水电能源科学, 2012,30(10):107-109,149. Wu J, Cui Y L, Zhao S J, ea al. Experiment of agricultural non-point source pollution mitigation by field drain and pond wetland[J]. WaterResources and Power, 2012,30(10):107-109,149.
[8]	Kroger R, Holland M M, Moore M T, et al. Hydrological variability and agricultural drainage ditch inorganic nitrogen reduction capacity[J]. Journal of Environmental Quality, 2007,36:1646-1652.
[9]	宁夏回族自治区生态环境厅.宁夏回族自治区环境质量公报[EB/OL].[2021-05-31] https://sthjt.nx.gov.cn/page/news/article/202105/202105311555545vOhoV.html. Department of Ecology and Environment of Ningxia Hui Autonomous Region. Ningxia Hui Autonomous Regionenvironmental quality bulletin[EB/OL].[2021-05-31] https://sthjt.nx.gov.cn/page/news/article/202105/202105311555545vOhoV.html.
[10]	Shan J, Zhao X, Sheng R, et al. Dissimilatory nitrate reduction processes in typical Chinese paddy soils:Rates, relative contributions, and influencing factors[J]. Environmental Science & Technology, 2016,50(18):9972-80.
[11]	Tan E, Zou W, Zheng Z, et al. Warming stimulates sediment denitrification at the expense of anaerobic ammonium oxidation[J]. Nature Climate Change, 2020,10(4):349-355.
[12]	史祯琦,房凯,周佳华,等.淹水底泥反硝化消纳面源氮潜力的影响因子分析[J]. 中国农村水利水电, 2022,(6):34-39. Shi Z Q, Fang K, Zhou J H, et al. Analysis of influencing factors on the potential of denitrification of flooded sediment to dissipate non-point source nitrogen[J]. China Rural Water and Hydropower, 2022,(6):34-39.
[13]	Hayakawa A, Nakata M, Jiang R, et al. Spatial variation of denitrification potential of grassland, windbreak forest, and riparian forest soils in an agricultural catchment in eastern Hokkaido, Japan[J]. Ecological Engineering, 2012,47(10):92-100.
[14]	Jia J X, Li Z P, Liu M, et al. Effects of glucose addition on N transformations in paddy soils with a gradient of organic C content in subtropical China[J]. Agricultural Sciences in China, 2010,9(9):1309-1316.
[15]	孙志高,刘景双,杨继松,等.三江平原典型小叶章湿地土壤硝化-反硝化作用与氧化亚氮排放[J]. 应用生态学报, 2007,(1):185-192. Sun Z G, Liu J S, Yang J S, et al. Nitrification-denitrification and N2O emission of typical Calamagrostis angustifolia wetland soils in Sanjiang Plain[J]. Chinese Journal of Applied Ecology, 2007,(1):185-192.
[16]	李如忠,王莉,刘超.巢湖滨岸水塘洼地沉积物反硝化速率及对外源碳氮的响应[J]. 环境科学, 2020,41(4):1684-1691. Li R Z, Wang L, Liu C. Sediment denitrification rate and its response to exogenous carbon and nitrogen in the ponds and bottomland of the Chaohu Lakeshore zone[J]. Environmental Science, 2020,41(4):1684-1691.
[17]	齐冉,张灵,杨帆,等.水力停留时间对潜流湿地净化效果影响及脱氮途径解析[J]. 环境科学, 2021,42(9):4296-4303. Qi R, Zhang L, YANG F, et al. Effect of hydraulic residence time on removal efficiency of pollutants in subsurface flow constructed wetlands and analysis of denitrification mechanism[J]. Environmental Science, 2021,42(9):4296-4303.
[18]	安芳娇,赵智超,黄利,等.HRT对厌氧氨氧化协同异养反硝化脱氮的影响[J]. 环境科学, 2018,39(9):4302-4309. An F J, Zhao Z C, Huang L, et al. Effect of HRT on nitrogen removal using ANAMMOX and heterotrophic denitrification[J]. Environmental Science, 2018,39(9):4302-4309.
[19]	王书伟,颜晓元,单军,等.利用膜进样质谱法测定不同氮肥用量下反硝化氮素损失[J]. 土壤, 2018,50(4):664-673. Wang S W, Yan X Y, Shan J, et al. Quantitative determination of N loss through denitrification under different N fertilizer application rates by membrane inlet mass spectrometry (MIMS)[J]. Soil, 2018,50(4):664-673.
[20]	李晓波,夏永秋,郎漫,等.N2:Ar法直接测定淹水环境反硝化产物N2的产生速率[J]. 农业环境科学学报, 2013,32(6):1284-1288. Li X B, Xia Y Q, Lang M, et al. N2:Ar technique for direct determination of denitrification rate of aquatic ecosystems using membrane inlet mass spectrometry[J]. Journal of Agro-Environment Science, 2013,32(6):1284-1288.
[21]	Soana E, Anna G,Vincenzi F, et al. Nitrate availability affects denitrification in Phragmites australis sediments[J]. Journal of Environmental Quality, 2020,49(1):194-209.
[22]	Zhang Z Q, Chen H Z, Mu X Y, et al. Nitrate application decreased microbial biodiversity but stimulated denitrifiers in epiphytic biofilms on Ceratophyllum demersum[J]. Journal of Environmental Management, 2020,269(C):110814.
[23]	Zhao W, Wang Y Y, Liu S H, et al. Denitrification activities and N2O production under salt stress with varying COD/N ratios and terminal electron acceptors[J]. Chemical Engineering Journal, 2013,215-216.
[24]	Ge S J, Peng Y Z, Wang S Y, et al. Nitrite accumulation under constant temperature in anoxic denitrification process:The effects of carbon sources and COD/NO3-N[J]. Bioresource Technology, 2012,114:137-143.
[25]	王伟,赵中原,张鑫,等.不同外碳源对尾水极限脱氮性能及微生物群落结构的影响[J]. 环境科学, 2022,43(9):4717-4726. Wang W, Zhao Z Y, Zhang X, et al. Effects of external carbon sources on ultimate nitrogen removal performance and microbial community in secondary effluent treating process[J/OL]. Environmental Science, 2022,43(9):4717-4726.
[26]	王维奇,王秀杰,李军,等.不同驯化方式实现SBR中部分反硝化的对比研究[J]. 中国环境科学, 2018,38(11):4085-4093. Wang W Q, Wang X J, Li J, et al. Comparative study on achieving partial denitrification in a SBR by different acclimation methods[J]. China Environmental Science, 2018,38(11):4085-4093.
[27]	Kindaichi T, Yamaoka S, Uehara R, et al. Phylogenetic diversity and ecophysiology of Candidate phylum Saccharibacteria in activated sludge.[J]. FEMS microbiology ecology, 2016,92(6):078.
[28]	魏琛,陆天友,钟仁超,等.HRT及氮素负荷对厌氧氨氧化系统的影响[J]. 环境科学学报, 2010,30(4):749-755. Wei C, Lu T Y, Zhong R C, et al. Infiuence of HRT and nitrogen load on an ANAMMOX system[J]. Acta Scientiae Circumstantiae, 2010, 30(4):749-755.
[29]	鲁彩艳,陈欣.不同施肥处理土壤及不同C/N比有机物料中有机N的矿化进程[J]. 土壤通报, 2003,(4):267-270. Lu C Y, Chen X. Mineralization process of soil organic nitrogen in different fertilizer systems and organic materials with different C/N Ratios[J]. Chinese Journal of Soil Science, 2003,(4):267-270.
[30]	付昆明,张晓航,刘凡奇,等.葡萄糖碳源条件下C/N对反硝化和N2O释放性能的影响[J]. 环境工程学报, 2021,15(4):1279-1288. Fu K M, Zhang X H, Liu F Q, et al. Effect of C/N on denitrification and N2O release with glucose as the carbon source[J]. Chinese Journal of Environmental Engineering, 2021,15(4):1279-1288.
[31]	李琳琳,李荣涛,孔维静,等.曝气人工湿地脱除低污染水中氮的影响因素[J]. 环境科学, 2021,42(12):5857-5864. Li L L, Li R T, Kong W J, et al. Influencing factors of nitrogen removal from low-pollution water by aerated constructed wetland[J]. Environmental Science, 2021,42(12):5857-5864.
[32]	韩焕豪.稻田-生态沟-塘堰湿地系统氮素迁移转化规律及机理研究[D]. 武汉:武汉大学, 2019. Han H H. Study on the law and mechanism of nitrogen transfer and transformation in paddy field eco-ditch wetland system[D]. Wuhan:Wuhan University, 2019.
[33]	黄潇.多级AO-深床滤池工艺深度处理城市污水效能及微生物特征[D]. 哈尔滨:哈尔滨工业大学, 2019. Huang X. Multistage A/O combined with deep bed filter process treating municipal wastewater:performance and microbial characteristics[D]. Harbin:Harbin Institute of Technology, 2019.
[34]	Md M R, Keryn L R, Michael R G, et al. Role of organic carbon, nitrate and ferrous iron on the partitioning between denitrification and DNRA in constructed stormwater urban wetlands[J]. Science of the Total Environment, 2019,666:608-617.
[35]	钟华,程莹,张洪维,等.基于玉米芯的固相反硝化柱实验研究[J]. 湖南大学学报(自然科学版), 2019,46(12):133-140. Zhong H, Cheng Y, Zhang H W, et al. Performance of corncob-based Solid Phase Denitrification System:A Column Study[J]. Journal of Hunan University (Natural Sciences), 2019,46(12):133-140.
[36]	Chutivisut P, Isobe K, Powtongsook S, et al. Distinct microbial community performing dissimilatory nitrate reduction to ammonium (DNRA) in a high C/NO3-reactor[J]. Microbes and environments, 2018,0:000-000.
[37]	赵凡,王洪浩,李京玲,等.运行时长和淹没高度对雨水生物滞留系统氮素去除的影响[J]. 节水灌溉, 2022,(4):16-20,26. Zhao F, Wang H H, Li J L, et al. The effects of operating time and submerged height on the removal of nitrogen in stormwater bioretention system[J]. Water Saving Irrigation, 2022,(4):16-20,26.
[38]	She D L, Zhang L, Gao X M, et al. Limited N removal by denitrification in agricultural drainage ditches in the Taihu Lake region of China[J]. Journal of soils and sediments, 2018,18(3):1110-1119.
[39]	Vymazal J, Březinová T D. Removal of nutrients, organics and suspended solids in vegetated agricultural drainage ditch[J]. Ecological Engineering, 2018,118:97-103.
[40]	周健,陈飞,张乐,等.进水方式对序批式人工湿地处理效能的影响[J]. 环境工程学报, 2012,6(11):3873-3876. Zhou J, Chen F, Zhang L, et al. Influence of flow modes on wastewater treatment of sequencing batch constructed wetlands[J]. Chinese Journal of Environmental Engineering, 2012,6(11):3873-3876.