Abstract:The sediments collected from different areas of Lake Taihu were anaerobically cultured, in combination with high-throughput sequencing and isotope enrichment technologies, to explore whether nitrate dependent ferrous [Fe(II)] oxidation (NDFO) can occur in the sediments of Lake Taihu, and whether Fe(II) addition can promote nitrogen removal from Lake Taihu. After anaerobic incubation for 24h, the concentrations of NO3−-N and Fe(II) in the overlying water decreased simultaneously, showing a significantly positive correlation (P<0.01). The relative abundance of NDFO related genera in cultured sediments increased from 0.69%±0.07% to 4.23%±0.90%. Combined with the change of sediment color, it can be inferred that NDFO occurred during anaerobic culture. The concentrations of NO3−-N in the control and the treatment added with lowest levels of Fe(II) (2.8mg) were significantly higher than those in other treatments (P<0.05, ANOVA), while the concentration of nitrite (NO2−) increased significantly with the increase of Fe(II) addition (P<0.01, ANOVA), indicating that a certain amount of Fe(II) can promote the reduction of NO3− and cause the accumulation of NO2−. After the addition of a small amount of Fe(II) (about 1.9mg/g sediment), the potential denitrification rate of the sediment in the different areas of Lake Taihu reached 213~252mg N/(m2·d), indicating that Fe(II) may play an important role in the denitrification process in Lake Taihu.
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