The effect mechanism of K-Birnessite on denitrification in sediments
XU Wei-mo1, ZHANG Long2, LI Run-jia3, CAO Li4, LONG Dan4, LIU Dan-yang1, ZHANG Li-pan1, AI Hai-nan1
1. Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, College of Environment and Ecology, Chongqing University, Chongqing 400044, China; 2. Youyang Municipal Ecology and Environment Bureau, Chongqing 409899, China; 3. Nanjing Municipal Design and Research Institute Co., Ltd, Nanjing 210008, China; 4. Chongqing Chenghuan Construction Engineering Co., Ltd, Chongqing 409819, China
Abstract：The effect mechanism of K-Birnessite on denitrification were investigated by exposing the sediments of Qingshuixi Section, a tributary of Jialing River, Chongqing, to different contents of δ-MnO2 (0,2,4,7g/kg). The findings revealed that δ-MnO2 inhibited the activity of microbial electron transport system (ETS) in the sediment, resulting in a decrease in key enzyme activities in the denitrification process [nitrate reductase (NAR): 9.62%~43.64%; nitrite reductase (NiRs): 29.13%~69.39%; nitric oxide reductase (NOR): 22.55%~44.87%; nitrous oxide reductase (NOS): 20.48%~68.80%, whereas inhibited the entire denitrification process, specially significantly decreased the sediment NO3--N reduction rate (23.47%~89.30%) in each experimental group during 0to 3h. Additionally, δ-MnO2 significantly decreased the ratio of NiRs/NOS, which may have contributed to the N2O release reduction (2.65%~28.55%) observed. The results of the microbiological investigation showed that δ-MnO2 alters the makeup of the microbial community, causing it to evolve towards an environment adapted to high Mn content. Manganese redox-related species were enriched, including Exiguobacterium_sibiricum_255-15 of Firmicutes, Bacillus simplex of Firmicutesand Pseudomonadales of Proteobacteria. However, the abundance of Trichococcus linked to sediment heterotrophic denitrification decreased, which may be the underlying cause for the deterioration of sediment denitrification performance after δ-MnO2 exposure.
徐炜谟, 张龙, 李润佳, 曹力, 龙旦, 刘丹阳, 张李潘, 艾海男. 水钠锰矿对沉积物反硝化过程的影响机制[J]. 中国环境科学, 2023, 43(7): 3704-3712.
XU Wei-mo, ZHANG Long, LI Run-jia, CAO Li, LONG Dan, LIU Dan-yang, ZHANG Li-pan, AI Hai-nan. The effect mechanism of K-Birnessite on denitrification in sediments. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3704-3712.
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