The driving effect of Bacillus megaterium-Celosia argentea L. combination on the succession of microbial communities in cadmium-contaminated soil
LAI Cai-xing1, LIN Hua1,2,3, LIU Ze-hui1, DONG Zi-han1, LIU Jie1, YU Guo1,4
1. Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541006, China; 2. Guangxi Collaborative Innovation Center for Water Pollution Control and water safety in Karst Area, Guilin University of Technology, Guilin 541006, China; 3. Guangxi Modern Industry College of Ecology and Environmental Protection, Guilin University of Technology, Guilin 541006, China; 4. Center for Water and Ecology, Tsinghua University, Beijing 100084, China
Abstract：This study focused on the plant-microbe combined remediation system involving Bacillus megaterium and Celosia argentea L., to explore the impact of Bacillus megaterium on the succession of the rhizosphere microbial community and its role in the remediation of cadmium-contaminated soil. High-throughput sequencing analysis was conducted to examine the structural changes in the rhizosphere microbiota of Celosia argentea at different time points (7th, 21st, and 50th days). The results indicated that in the treatment group, inoculated with B. megaterium, the number of OTUs, diversity indices (Shannon, Simpson), and richness indices (Chao1, Ace) of the microbial community were all higher than those in the control group by the 50th day; the Acidobacteriota, Chloroflexi, Proteobacteria, and Bacteroidetes were the core groups within the microbial community; B. megaterium was able to dominate the rhizosphere microbial community in the early stages but its relative abundance gradually declined from 12.01% to 1.17% over the 50days; Functional prediction of the soil microbial community showed that B. megaterium mainly promoted the C and N cycle within the microbial community, potentially exerting a positive influence on the functionality and stability of the microbial community; B. megaterium significantly increased the cadmium content in the leaves of C. argentea and the bioavailable cadmium content in the rhizosphere soil by 40.3% and 17.6%, respectively. This study provides a theoretical foundation and empirical data support for optimizing plant-microbe combined remediation techniques and understanding the succession patterns of microbial communities in plant-microbe combined remediation systems.
赖才星, 林华, 刘泽蕙, 董梓涵, 刘杰, 俞果. 巨大芽孢杆菌-青葙组合对镉污染土壤微生物群落演替的驱动作用[J]. 中国环境科学, 2025, 45(2): 1036-1044.
LAI Cai-xing, LIN Hua, LIU Ze-hui, DONG Zi-han, LIU Jie, YU Guo. The driving effect of Bacillus megaterium-Celosia argentea L. combination on the succession of microbial communities in cadmium-contaminated soil. CHINA ENVIRONMENTAL SCIENCECE, 2025, 45(2): 1036-1044.
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