Simultaneous nitrification and denitrification of aerobic granular sludge for nitrogen and phosphorus removal
LI Dong1, LI Yue1, LI Yu-meng1, YANG Jing-wei2, ZHANG Jie1,3
1. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124; 2. Beijing Municipal Engineering Design and Research Institute Co. Ltd., Beijing 100086, China; 3. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
Abstract:Flocculent sludge was inoculated at room temperature in SBR reactors R1, R2, and R3 running in gradient, fast, and slow feeding, respectively. Using artificial water distribution as the influent substrate, the impact of the feeding method on the carbon source storage performance and pollutant removal effect was explored. The results showed that the gradient feeding had better internal carbon source storage performance and denitrification effect. After successful startup, the CODin, SNED rates and the average removal rates of TN, COD and TP in R1 were 99.69%, 81.52%, 79.07%, 92.35%, 96.03%, respectively. The average removal rate of TP was ond only to 98.43% of R3. This was because the contribution of PAO to the internal carbon source storage(Ppaos) under slow feeding was the majority(54.41%), while the gradient water Ppaos was only 47.90%. The sludge concentration in R1 was 5 mg/L, which was lower than the 6389 mg/L in R2, but its MLSS/MLVSS was 0.90, indicating that the gradient influent granular sludge had a higher biomass. The EPS analysis results showed that the composition of extracellular polymeric substances(EPS) changed with the change of the feeding method, and the gradient feeding contained higher PN, so the particles were more hydrophobic and the particle structure was more stable.
李冬, 李悦, 李雨朦, 杨敬畏, 张杰. 好氧颗粒污泥同步硝化内源反硝化脱氮除磷[J]. 中国环境科学, 2022, 42(3): 1113-1119.
LI Dong, LI Yue, LI Yu-meng, YANG Jing-wei, ZHANG Jie. Simultaneous nitrification and denitrification of aerobic granular sludge for nitrogen and phosphorus removal. CHINA ENVIRONMENTAL SCIENCECE, 2022, 42(3): 1113-1119.
高景峰,王时杰,樊晓燕,等.同步脱氮除磷好氧颗粒污泥培养过程微生物群落变化[J].环境科学, 2017,38(11):4696-4705.Gao J F, Wang S J, Fan X Y, et al. Microbial population dynamics during sludge granulation in a simultaneous nitrogen and phosphorus removal system[J]. Environmental Science, 2017,38(11):4696-4705.
[2]
鲁磊,信欣,鲁航,等.连续流好氧颗粒污泥系统处理低COD/N实际生活污水的工艺优化[J].环境科学, 2015,36(10):3778-3785.Lu L, Xin X, Lu H, et al. Process optimization of aerobic granular sludge continuous-flow system for the treatment of low COD/N ratio sewage[J]. Environmental Science, 2015,36(10):3778-3785.
[3]
Javier G, Albert G, Juan A B. The nature of the carbon source rules the competition between PAO and denitrifiers in systems for simultaneous biological nitrogen and phosphorus removal[J]. Water Research,2011,45(16):4793-4802.
[4]
de Kreuk M K, van Loosdrecht M C M. Selection of slow growing organisms as a means for improving aerobic granular sludge stability[J]. Water science and technology:a journal of the International Association on Water Pollution Research, 2004,49(11/12):9-17.
[5]
Beun J J, Verhoef E V, Van Loosdrecht M C, et al. Stoichiometry and kinetics of poly-beta-hydroxybutyrate metabolism under denitrifying conditions in activated sludge cultures[J]. Biotechnology and Bioengineering, 2000,68(5):496-507.
[6]
崔有为,王好韩,金常林.好氧丰盛/缺氧饥饿模式驯化微生物积累内源聚合物并实现内源反硝化[J].北京工业大学学报, 2020,46(11):1283-1290.Cui Y W, Wang H H, Jin C L. Selection of endogenous polymers accumulating bacteria by aerobic feast/anoxic famine mode and realizing denitrification by endogenous polymers[J]. Journal of Beijing University of Technology, 2020,46(11):1283-1290.
[7]
季强.水质检测与分析[M].北京:中国建筑工业出版社, 2015.Ji Q. Water quality detection and analysis[M]. Beijing:China Construction Industry Press, 2015.
[8]
Li X Y, Yang S F. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge[J]. Water Research, 2007,41(5):1022-1030.
[9]
Frolund B, Griebe T, Nielsen P H. Enzymatic-activity in the activated-sludge floc matrix[J]. Applied Microbiology and Biotechnology, 1995,43(4):755-761.
[10]
Loewus F A. Improvement in anthrone method for determination of carbohydrates[J]. Analytical Chemistry, 1952,24(1):219-219.
[11]
都叶奇,于德爽,甄建园,等.进水C/N对SNEDPR系统脱氮除磷的影响[J].环境科学, 2019,40(2):816-822.Du Y Q, Yu D S, Zhen J Y, et al. Effect of influent C/N ratio on the nutrient removal characteristics of SNEDPR systems[J].Environmental Science, 2019,40(2):816-822.
[12]
Xiaoxia W, Shuying W, Tonglai X, et al. Treating low carbon/nitrogen (C/N) wastewater in simultaneous nitrification-endogenous denitrification and phosphorous removal (SNDPR) systems by strengthening anaerobic intracellular carbon storage[J]. Water Research, 2015,77:191-200.
[13]
GB18918-2002城镇生活污水厂污染物排放标准[S].GB18918-2002 Discharge standard of pollutants for urban domestic sewage plants[S].
[14]
Oliver T I, Mohamed S Z, Rania A H, et al. Long-term aerobic granular sludge stability through anaerobic slow feeding, fixed feast-famine period ratio, and fixed SRT[J]. Journal of Environmental Chemical Engineering, 2020,8(2),doi:10.1016/j.jece.2020.103681.
[15]
T. R, C. K, B. H, et al. Comparison of two different anaerobic feeding strategies to establish a stable aerobic granulated sludge bed[J]. Water Research, 2013,47(17):6423-6431.
[16]
Gregory R C, Jillian F B, Jürg K, et al. Glycogen-accumulating organisms in laboratory-scale and full-scale wastewater treatment processes b b The GenBank accession numbers for the sequences reported in this paper are given in Methods[J]. Microbiology,2002,148(11):3353-3364.
[17]
Hong-Bo C, Qi Y, Xiao-Ming L,et al. Post-anoxic denitrification via nitrite driven by PHB in feast-famine sequencing batch reactor[J].Chemosphere, 2013,92(10):1349-1355.
[18]
Liu Y, Tay J. Influence of starvation time on formation and stability of aerobic granules in sequencing batch reactors[J]. Bioresource Technology, 2008,99(5):980-985.
[19]
Jun L, Ang C, Libin D, et al. Aerobic sludge granulation in a Reverse Flow Baffled Reactor (RFBR) operated in continuous-flow mode for wastewater treatment[J]. Separation and Purification Technology,2015,149:437-444.
[20]
Kent T R, Bott C B, Wang Z. State of the art of aerobic granulation in continuous flow bioreactors[J]. Biotechnology Advances, 2018,36(4):1139-1166.
[21]
李冬,杨敬畏,李悦,等.控制回流比及有机负荷实现交替厌氧/好氧连续流好氧颗粒污泥系统的稳定运行[J].中国环境科学,2021,41(9):4149-4156.Li D, Yang J W, Li Y, et al. Control reflux ratio and organic load to achieve stable operation of alternate anaerobic/aerobic aerobic granular sludge continuous flow system[J]. China Environmental Science, 2021,41(9):4149-4156.
[22]
Yu L, Joo-Hwa T. State of the art of biogranulation technology for wastewater treatment[J]. Biotechnology Advances, 2004,22(7):533-563.
[23]
Yanghui X, Yu L. Importance of extracellular proteins in maintaining structural integrity of aerobic granules[J]. Colloids and Surfaces B:Biointerfaces, 2013,112:435-440.
[24]
Diane M M, Elizabeth W B, Paul K W, et al. Spectrofluorometric characterization of dissolved organic matter for indication of precursor organic material and aromaticity[J]. Limnology and Oceanography,2001,46(1):38-48.
[25]
Li D, Yang J, Li Y, et al. Research on rapid cultivation of aerobic granular sludge (AGS) with different feast-famine strategies in continuous flow reactor and achieving high-level denitrification via utilization of soluble microbial product (SMP)[J]. Science of the Total Environment, 2021,786,doi:10.1016/J.Scitotenv.2021.147237.