In this study,soil samples from the reclaimed land filled with coal gangue (SS) with different reclamation years (2015,2010,and 2001 years) in Xuzhou mining area and from normal farmlands which were not affected by coal mining subsidence (SSC) were collected.The sequence numbers of bacterial community were determined by the Illumina PE250sequencing method,and the vertical structure and time structure were analyzed.The results showed that:(1) Reclamation decreased the number of bacteria species and the community diversity,but the goodness between SS and SSC increased with the age of reclamation.(2)Firmicutes and Proteobacteria dominated in SS at the phylum level,the number of Firmicutes increased after reclamation and Firmicutes also likely transferred from 20~40cm to 0~20cm soil layer.(3)Bacilli predominantly existed in soils at the class level,the number of Bacilli in 0~20cm soil layer was higher in SS than in SSC samples,and the number in 20~40cm soil layers of SS samples decreased with the increase of the reclamation years.(4)Lactobacillales and Bacillales dominated in SS at the order level.Desulfuromonadales played an important role in the remediation of heavy metal pollution and its number in 0~20cm soil layer of SS was 74.81~99.59% less than that in SSC samples.(5) In SS samples,Bacillus,Enterococcus and Streptococcaceae were the dominant family,while Bacillus,Enterococcus and Lactococcus were the dominant genus,and Bacillus sp.JH7,Enterococcus faecium and Lactococcus piscium were the dominant species.All of these in SS samples were less in number than SSC samples especially in 0~20cm soil layer,while the number in 20~40cm soil layer of SS decreased with the increase of the reclamation years.(6) Dehydrogenase activity was negatively correlated with the number of Firmicutes.spp,but was positively correlated with the number of Actinobacteria and the number of Gammaproteobacteria.Also,the type of dominant soil bacteria did not change,but their quantitative structure varied over time.Owing to Firmicutes are suitable for growth in the absence of water and extreme environments,but Proteobacteria is conducive to soil nitrogen and energy cycling.Therefore,the soil quality was improved by adjusting soil bacterial community structure and shorten the recovery period.
侯湖平, 王琛, 李金融, 丁忠义, 张绍良, 黄磊, 董健, 马静, 杨永均. 煤矸石充填不同复垦年限土壤细菌群落结构及其酶活性[J]. 中国环境科学, 2017, 37(11): 4230-4240.
HOU Hu-ping, WANG Chen, LI Jin-rong, DING Zhong-yi, ZHANG Shao-liang, HUANG Lei, DONG Jian, MA Jing, YANG Yong-jun. Variation of bacterial community structure and enzyme activities in reclaimed soil filled with coal gangues along a relamation chronosequence. CHINA ENVIRONMENTAL SCIENCECE, 2017, 37(11): 4230-4240.
Poncelet D M, Cavender N, Cutright T J, et al. An assessment of microbial communities associated with surface mining-disturbed overburden[J]. Environmental Monitoring & Assessment, 2014,186(3):1917-1929.
Luna L, Pastorelli R, Bastida F, et al. The combination of quarry restoration strategies in semiarid climate induces different responses in biochemical and microbiological soil properties[J]. Applied Soil Ecology, 2016,107:33-47.
Dangi S R, Stahl P D, Wick A F, et al. Soil microbial community recovery in reclaimed soils on a surface coal mine site[J]. Soil Science Society of America Journal, 2012,76(3):915-924.
Li J, Zheng Y, Yan J, et al. Effects of different regeneration scenarios and fertilizer treatments on soil microbial ecology in reclaimed opencast mining areas on the Loess Plateau, China[J]. PLoS one, 2013,8(5):e63275.
[12]
Zornoza R, Acosta J A, Faz A, et al. Microbial growth and community structure in acid mine soils after addition of different amendments for soil reclamation[J]. Geoderma, 2016,272:64-72.
Rojas C, Gutierrez R M, Bruns M A. Bacterial and eukaryal diversity in soils forming from acid mine drainage precipitates under reclaimed vegetation and biological crusts[J]. Applied Soil Ecology, 2016,105:57-66.
[16]
Li Y, Chen L, Wen H. Changes in the composition and diversity of bacterial communities 13years after soil reclamation of abandoned mine land in eastern China[J]. Ecological Research, 2015,30(2):357-366.
Amato K R, Yeoman C J, Kent A, et al. Habitat degradation impacts black howler monkey (Alouatta pigra) gastrointestinal microbiomes[J]. The ISME Journal, 2013,7(7):1344-1353.
[21]
Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur:Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities[J]. Applied Environmental Microbiology, 2009,75(23):7537-7541.
Lozupone C, Lladser M E, Knights D, et al. UniFrac:an effective distance metric for microbial community comparison[J]. The ISME Journal, 2011,5(2):169-172.
Kim H J, Eom H J, Park C, et al. Calcium carbonate precipitation by bacillus and sporosarcina strains isolated from concrete and analysis of the bacterial community of concrete[J]. Journal of Microbiology and Biotechnology, 2016,26(3):540-548.
[35]
Maia O B, Duarte R, Silva A M, et al. Evaluation of the components of a commercial probioticing not blobioticmice experimently challenged with Salmonellaenterica sub sp.enterica ser. Typhimurium[J]. Veterinary Microbiology, 2001,79(2):183-189.
