Determination of the total phosphorus content in activated sludge by ultrasonic pretreatment assisted wet method
ZENG Shang-jing1,2, PENG Jia-xi1,2, SUN Xue-jian2,3, WANG Fan1,2, AI Sheng-shu1,2, BIAN De-jun1,2
1. School of Water Conservancy & Environment Engineering, Changchun Institute of Technology, Changchun 130012, China; 2. Jilin Provincial Key Laboratory of Municipal Wastewater Treatment, Changchun Institute of Technology, Changchun 130012, China; 3. School of Environment, Northeast Normal University, Changchun 130117, China
Abstract：The total phosphorus (TP) content of activated sludge was determined by an ultrasonic cracking assisted wet method. This study investigated the individual and interactive effects between three factors of sampling volume, sonication time, and sonication power, using the response surface methodology with the TP content as the response value, based on the single-factor test. Meanwhile, four sludge TP determination methods were compared, i.e., ultrasonic cracking wet method, microwave digestion method, SMT method, and improved national standard method. The results showed that the ultrasonic cracking wet method had the highest accuracy and stability in determining the TP content of sludge under the conditions of 15mL of sludge mixture sampling volume, 22min of ultrasonic time, and 720W of ultrasonic power. The results of the sludge TP determination followed the order of ultrasonic cracking wet method > microwave digestion method > SMT method > improved national standard method. There was a significant linear correlation between the ultrasonic cracking wet method and the other three methods (correlation coefficients of 0.9997, 0.9977, and 0.9969, respectively) with the fitted function slope values of 0.9436, 0.9183, and 0.8295, respectively. The RSDs for the determination results of TP in sludge samples by the ultrasonic cracking wet method were £3.6% and the spiked recoveries were 96.5%~104.8%. This study demonstrates that the ultrasonic cracking wet method is of good reliability, economy, and ease of operation in determining TP. Furthermore, ultrasound can save chemicals and extraction time.
曾尚景, 彭加曦, 孙雪健, 王帆, 艾胜书, 边德军. 超声破解辅助湿法测定活性污泥中总磷含量[J]. 中国环境科学, 2023, 43(7): 3499-3508.
ZENG Shang-jing, PENG Jia-xi, SUN Xue-jian, WANG Fan, AI Sheng-shu, BIAN De-jun. Determination of the total phosphorus content in activated sludge by ultrasonic pretreatment assisted wet method. CHINA ENVIRONMENTAL SCIENCECE, 2023, 43(7): 3499-3508.
Sevil C, Büşra K, Büşra K, et al. Biological recovery of phosphorus from waste activated sludge via alkaline fermentation and struvite biomineralization by Brevibacterium antiquum [J]. Biodegradation, 2022,2(33):195-206.
[2]
周广强,谢 英,吴剑斌,等.基于WRF-Chem模式的华东区域PM2.5预报及偏差原因 [J]. 中国环境科学, 2016,36(8):2251-2259. Zhou G Q, Xie Y, Wu J B, et al. WRF-Chem based PM2.5 forecast and bias analysis over the East China Region [J]. China Environmental Science, 2016,36(8):2251-2259.
[3]
Wu G, Zeng W, Li S, et al. Phosphorus recovery from waste activated sludge by sponge iron seeded crystallization of vivianite and process optimization with response surface methodology [J]. Environmental Science and Pollution Research International, 2021,28(41):58375-58386.
[4]
Zan F X, Huang H, Guo G, et al. Sulfite pretreatment enhances the biodegradability of primary sludge and waste activated sludge towards cost-effective and carbon-neutral sludge treatment [J]. Science of the Total Environment, 2021,780(0):146634.
[5]
Xu X Z, Cao D, Wang Z H, et al. Study on ultrasonic treatment for municipal sludge [J]. Ultrasonics Sonochemistry, 2019,57:29-37.
[6]
刘 环.城镇污泥处置方案分析研究 [D]. 天津:天津大学, 2017. Liu H. Analysis and research on urban sludge disposal scheme [D]. Tianjin: Tianjin University, 2017.
[7]
冯士龙.城市污水厂污泥中磷的形态分布和释放特性研究 [D]. 南京:河海大学, 2008. Feng S L. Morphological distribution and release characteristics of phosphorus in municipal wastewater plant sludge [D]. Nanjing: Hohai University, 2008.
[8]
周旭红,曹晓辉.污泥中总磷测定方法的探讨 [J]. 浙江化工, 2005,36(2):43-44. Zhou X H, Cao X H. Discussion on the determination method of total phosphorus in sludge [J]. Zhejiang Chemical Industry, 2005,36(2):43-44.
[9]
陈国梅.钼酸铵分光光度法测定城市污泥中的总磷 [J]. 中国给水排水, 2006,22(2):85-86. Chen G M. Determination of total phosphorus in municipal sludge by ammonium molybdate spectrophotometry [J]. China Water & Wastewater, 2006,22(2):85-86.
[10]
蒯丽君,郝 俊,孔详科,等.微波消解-磷钼蓝分光光度法测定城市污泥中总磷浓度 [J]. 中国无机分析化学, 2019,9(5):25-29. Kuai L J, Hao J, Kong X K, et al. Microwave digestion-phosphorus molybdenum blue spectrophotometric determination of total phosphorus concentration in municipal sludge [J]. Chinese Journal of Inorganic Analytical Chemistry, 2019,9(5):25-29.
[11]
Saktaywin W, Tsuno H, Nagare H, et al. Advanced sewage treatment process with excess sludge reduction and phosphorus recovery [J]. Water Research, 2004,39(5):902-910.
[12]
Wang S G, Jin X C, Pang Y, et al. Phosphorus fractions and phosphate sorption characteristics in relation to the sediment compositions of shallow lakes in the middle and lower reaches of Yangtze River region, China [J]. Journal of Colloid And Interface Science, 2005,289(2):339-346.
[13]
冯新长,张黎黎.污水处理厂剩余污泥中总磷的测定 [J]. 环境科学导刊, 2012,31(3):94-96. Feng X C, Zhang L L. Determination of total phosphorus in residual sludge of sewage treatment plant [J]. Environmental Science Guide, 2012,31(3):94-96.
[14]
路兴和,丁 煜,吴庆华.硝酸镁消解分光光度法测定城市污泥中的总磷 [J]. 给水排水, 2016,52(S1):247-249. Lu X H, Ding Y, Wu Q H. Spectrophotometric determination of total phosphorus in municipal sludge with magnesium nitrate digestion [J]. Water & Wastewater, 2016,52(S1):247-249.
[15]
Guo J X, Liu B F, Wang Q L, et al. Ultrasonic waste activated sludge disintegration for recovering multiple nutrients for biofuel production [J]. Water Research, 2016,93:56-64.
[16]
郭 璇,杨艳玲,李 星,周志伟,马长红,张洋等.超声空化及絮体破碎过程的模拟与试验分析 [J]. 中国环境科学, 2015,35(5):1429-1435. Guo X, Yang Y L, Li X, et al. Simulation of ultrasound cavitation bubble and numerical/experimental analysis of flocs breakage process [J]. China Environmental Science, 2015,35(5):1429-1435.
[17]
Merouani S, Hamdaoui O, Rezgui Y, et al. Theoretical estimation of the temperature and pressure within collapsing acoustical bubbles [J]. Ultrasonics Sonochemistry, 2014,21(1):53-59.
[18]
GB 11893-89 水质总磷的测定钼酸铵分光光度法 [S]. GB 11893-89 Determination of total phosphorus in water by ammonium molybdate spectrophotometry [S].
[19]
仉铭坤,杜明阳,郭彦青,等.响应面法优化O3/US-混凝耦合去除皮革废水中的磷 [J]. 环境科学学报, 2020,40(4):1185-1195. Zhang M K, Du M Y, Guo Y Q, et al. Optimized O3 /US-coagulation coupling using response surface methodology forphosphorus removal in tannery wastewater [J]. Acta Scientiae Circumstantiae, 2020,40(4): 1185-1195.
[20]
徐慧敏,秦卫华,何国富,等.超声联合热碱技术促进剩余污泥破解的参数优化 [J]. 中国环境科学, 2017,37(9):3431-3436. Xu H M, Qin W H, He G F, et al. Optimization of combined ultrasonic and thermo-chemical pretreatment of waste activated sludge for enhanced disintegration [J]. China Environmental Science, 2017,37(9): 3431-3436.
[21]
王 怡,刘 潘,彭党聪.超声及碱处理促进剩余污泥水解的试验研究 [J]. 中国给水排水, 2010,26(15):28-31. Wang Y, Liu P, Peng D C. Experimental study on the promotion of residual sludge hydrolysis by ultrasound and alkali treatment [J]. China Water & Wastewater, 2010,26(15):28-31.
[22]
Guan S, Deng F, Huang S Q, et al. Optimization of magnetic field-assisted ultrasonication for the disintegration of waste activated sludge using Box-Behnken design with response surface methodology [J]. Ultrasonics Sonochemistry, 2017,1(38):9-18.
[23]
Guo S D, Qu F S, Ding A, et al. Effects of agricultural waste-based conditioner on ultrasonic-aided activated sludge dewatering [J]. RSC Advances, 2015,5(54):43065-43073.
Nguyen D D, Yoon Y S, Nguyen N D, et al. Enhanced efficiency for better wastewater sludge hydrolysis conversion through ultrasonic hydrolytic pretreatment [J]. Journal of the Taiwan Institute of Chemical Engineers, 2017,71:244-252.
[26]
Yu Chen, Lin Hao, Dong Mei Wang. Effect of ultrasonic energy density of ultrasonic sludge hydrolysis [J]. Advanced Materials Research, 2015,3848(1092-1093).
[27]
黄翔峰,朱其玮,申昌明,等.剩余污泥超声提取液培养生物破乳菌Alcaligenes sp.S-XJ-1 [J]. 中国环境科学, 2014,34(2):424-430. Huang F X, Zhu Q W, Shen C M, et al. Cultivation of demulsifying bacteria Alcaligenes sp. S-XJ-1using ultrasonic pretreated excess sludge [J]. China Environmental Science, 2014,34(2):424-430.
[28]
Guo H X, Felz S, Lin YM, et al. Structural extracellular polymeric substances determine the difference in digestibility between waste activated sludge and aerobic granules [J]. Water Research, 2020,181: 115924.
[29]
Jiang Y, Zhu S, Ouyang K, et al. Effect of ultrasonic cracking on nutrients and particle size of excess sludge(Article) [J]. Chinese Journal of Environmental Engineering, 2018,12(5):1303-1309.
[30]
Zhao Wei, Zhan X Hianhui, Liu W Leilin, et al. Research on ultrasonic treatment in the field of actual excess sludge treatment [J]. IOP Conference Series: Earth and Environmental Science, 2021,651(4): 042046.
[31]
Guo X, Liu J, Xiao B. Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge [J]. Journal of Biotechnology, 2014,188(1):130-135.
[32]
Wang F, Lu S, Ji M. Components of released liquid from ultrasonic waste activated sludge disintegration [J]. Ultrasonics Sonochemistry, 2006,13(4):334-338.
[33]
张 浩,杨艳玲,李 星,等.超声声能密度对净水厂污泥脱水性能的影响 [J]. 中国环境科学, 2017,37(3):1009-1015. Zhang H, Yang Y L, Li X, et al. Effect of ultrasound energy density on dewaterability of drinking water treatment sludge [J]. China Environmental Science, 2017,37(3):1009-1015.
[34]
Song Y D, Hu H Y, Zhou Y X. Lysis of stationary-phase bacterial cells by synergistic action of lytic peptidase and glycosidase from thermophiles [J]. Biochemical Engineering Journal, 2011,52(1):44-49.
[35]
Matnez E J, Roasa J G, Mran A, et al. Effect of ultrasound pretreatment on sludge digestion and dewatering characteristics: Application of particle size analysis [J]. Water, 2015(7):6583-6495.
[36]
Sun Loh Pei, Yu Ying Chen, Alnoor Hussien I brahim M, et al. Comparative study on the elucidation of sedimentary phosphorus species using two methods, the SMT and SEDEX methods [J]. Journal of analytical methods in chemistry, 2020,(1):92020.
